Disease relevance of Entpd5

• Alkaline phosphatase, 5'-nucleotidase nucleoside diphosphatase and thiamine pyrophosphatase activities were studied by cytochemical method applied to electron microscopy of brain microvasculature in normal and scrapie infected mice [1].

High impact information on Entpd5

• Glycoprotein reglucosylation and nucleotide sugar utilization in the secretory pathway: identification of a nucleoside diphosphatase in the endoplasmic reticulum [2].
• Mice were injected i.v. with Monastral blue 2 h prior to sacrifice to identify "activated" monocytes, and their isolated retinae were incubated with the Griffonia simplicifolia (GS) lectin or reacted for the nucleoside diphosphatase enzyme to visualize microglia and the vasculature [3].
• These data should prove valuable in designing studies to define the cellular function of the Pcph proto-oncogene [4].
• The mouse Pcph proto-oncogene was mapped by linkage analysis to within 3.3+/-2.3 cM of Pkch-rs1 on chromosome 12 [4].
• The single open reading frame identified in the cDNA sequence encoded a protein predicted to have 428 amino acids, which shared 93.7% amino acid identity with the Syrian hamster Pcph within the first 412 residues but had a shorter, highly dissimilar C-terminus [4].

Biological context of Entpd5

• Nucleoside diphosphatase (NDPase) activity was studied by electron microscope cytochemistry in surgical specimens obtained from aged human cerebral cortices [5].

Anatomical context of Entpd5

• We report here the isolation and characterization of a cDNA from mouse thymus encoding the murine homolog of the protein product of the Syrian hamster Pcph proto-oncogene [4].
• Nucleoside diphosphatase (NDPase) activity, using inosine-5'-diphospbate (IDP) as a substrate, was studied by light and electron microscope cytochemistry in brain and spinal cord of normal mouse [6].
• Plasmalemma-bound nucleoside diphosphatase as a cytochemical marker of central nervous system (CNS) mesodermal cells [6].
• Nucleoside diphosphatase (IDPase), localized using inosine diphosphate as substrate, allows the selective staining of blood vessels and cells of vascular origin, such as macrophages and microglia, whereas the neuroglial, the neuronal and the pigment epithelial cells remain unstained [7].
• The delimiting membranes of these structures possess AP, 5'-nucleotidase, nucleoside diphosphatase and Na+, K+-ATPase activities [8].

Associations of Entpd5 with chemical compounds

• Potential enzyme cytochemical markers for the axonal ER included glucose-6-phosphatase (G6Pase), thiamine pyrophosphatase, nucleoside diphosphatase, and acid hydroxylase activities [9].

Other interactions of Entpd5

• Microglial cells were identified by immunostaining for Mac-1, a monoclonal antibody to the CR3 complement receptor, and by histochemical staining for nucleoside diphosphatase (NDPase) [10].
• In this study, we examined the morphology and distribution of microglial cells in spinal cord sections from jimpy and normal mice at 10-12 and 20-22 days postnatal using a specific microglial marker, the nucleoside diphosphatase staining [11].

Analytical, diagnostic and therapeutic context of Entpd5

• The 1D4 antigen had multiple isoelectric points, the pattern of the bands detected on isoelectric focusing gel being quite similar to that of Type B nucleoside diphosphatase of the brain [12].

References