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Gene Review

Flot2  -  flotillin 2

Mus musculus

Synonyms: AI573412, ESA, Epidermal surface antigen, Esa, Esa1, ...
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High impact information on Flot2

  • At the cellular level, flotillin-2 was ubiquitously expressed, whereas flotillin-1 was most abundant in A498 kidney cells, muscle cell lines, and fibroblasts [1].
  • However, both nude and control mice were found to have a second Esa mRNA transcript that conserves amino acid sequence and molecular weight [2].
  • Medium size complexes were enriched in PrP, whereas small complexes contained non-raft proteins, PrP, and some ESA [3].
  • Large complexes were enriched in caveolin and ESA [3].
  • Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation [4].

Biological context of Flot2

  • Stable transfection of ESA/flotillin 2 fusion protein in cos cells induced filopodia formation and changed epithelial cells to a neuronal appearance [4].
  • Reggie-1/flotillin-2 is enriched in detergent insoluble (TX100) membrane fractions (DIG), co-localizes with activated GPI-linked proteins and the fyn-kinase in neurons and T cells, and thus apparently participates in the assembly of protein complexes essential for signal transduction [5].

Anatomical context of Flot2

  • Thus, the function of flotillin 2 may resemble that of the goldfish optic nerve neuronal regeneration proteins, Reggie 1 and 2 [4].
  • ECS-1, a monoclonal antibody (MoAb) raised to cultured human keratinocytes, stains the intercellular glycocalyx with a pemphigus-like pattern and recognizes a 35-kDa epidermal surface antigen (ESA) on Western blotting of keratinocyte extracts [4].
  • Here, we show that reggie-1/flotillin-2 is expressed throughout B cell development, as well as in primary B cells, purified by cell sorting [5].
  • On non-activated mature B cell Raji cell line we found reggie-1/flotillin-2 are exclusively in the detergent (TX100) insoluble membrane fractions that are staining positive for the raft marker GM1 [5].
  • Immunofluorescence microscopy showed that reggie-1/flotillin-2 is localized at the plasma membrane and marks intracellular spots in PBMCs [5].

Associations of Flot2 with chemical compounds

  • Epithelial surface antigen (ESA) is a glycoprotein with a distribution in vivo that is largely confined to human epithelial cells [6].
  • Tca and Esa are assigned provisionally to the first short-consensus repeat (SCR), UMC to the second SCR, Dra to the third SCR and Cra, WESa and WESb to the fourth SCR or to the serine/threonine rich region of the DAF protein [7].

Analytical, diagnostic and therapeutic context of Flot2


  1. Flotillins/cavatellins are differentially expressed in cells and tissues and form a hetero-oligomeric complex with caveolins in vivo. Characterization and epitope-mapping of a novel flotillin-1 monoclonal antibody probe. Volonte, D., Galbiati, F., Li, S., Nishiyama, K., Okamoto, T., Lisanti, M.P. J. Biol. Chem. (1999) [Pubmed]
  2. Epidermal surface antigen (MS17S1) is highly conserved between mouse and human. Cho, Y.J., Chema, D., Moskow, J.J., Cho, M., Schroeder, W.T., Overbeek, P., Buchberg, A.M., Duvic, M. Genomics (1995) [Pubmed]
  3. Both raft- and non-raft proteins associate with CHAPS-insoluble complexes: some APP in large complexes. Rouvinski, A., Gahali-Sass, I., Stav, I., Metzer, E., Atlan, H., Taraboulos, A. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  4. Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. Hazarika, P., Dham, N., Patel, P., Cho, M., Weidner, D., Goldsmith, L., Duvic, M. J. Cell. Biochem. (1999) [Pubmed]
  5. The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs. Solomon, S., Masilamani, M., Rajendran, L., Bastmeyer, M., Stuermer, C.A., Illges, H. Immunobiology (2002) [Pubmed]
  6. Biochemical analysis of a human epithelial surface antigen: differential cell expression and processing. Thampoe, I.J., Ng, J.S., Lloyd, K.O. Arch. Biochem. Biophys. (1988) [Pubmed]
  7. Use of the MAIEA technique to confirm the relationship between the Cromer antigens and decay-accelerating factor and to assign provisionally antigens to the short-consensus repeats. Petty, A.C., Daniels, G.L., Anstee, D.J., Tippett, P. Vox Sang. (1993) [Pubmed]
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