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Gene Review

Mbd1  -  methyl-CpG binding domain protein 1

Mus musculus

Synonyms: Cxxc3, Methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1
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Disease relevance of Mbd1

  • The defect was CD8 T cell intrinsic, because memory cell development was also delayed when MBD2(-/-) CD8 T cells were adoptively transferred into SCID mice [1].
  • The role of MBD2 during the differentiation of naive CD8 T cells into effector and memory cells was determined following acute infection of MBD2-deficient mice with lymphocytic choriomeningitis virus [1].

Psychiatry related information on Mbd1

  • Thus, these functional assays are useful to evaluate the consequences of mutation in the methyl-CpG-binding domain of MeCP2 and provide an insight into the relationship between the genotype and the severity of Rett syndrome [2].

High impact information on Mbd1


Biological context of Mbd1

  • These data support the hypothesis that MBD protein-associated histone deacetylase/chromatin-remodelling complexes are recruited to the parental allele that has methylated DNA and H3-K9 methylation, and are prevented from binding to the opposite allele by H3 lysine-4 methylation [6].
  • Missense mutations in the methyl-CpG-binding domain were analyzed by the transfection in mouse L929 cells and Drosophila SL2 cells [2].
  • The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1 [7].
  • MBD1 is a vertebrate methyl-CpG binding domain protein (MBD) that can bring about repression of methylated promoter DNA sequences [8].
  • NMR studies have revealed that the MBD adopts a wedge-shaped molecular structure [9].

Anatomical context of Mbd1

  • These data demonstrate that MBD2 is a previously unrecognized intracellular factor required for the efficient generation of protective memory CD8 T cells [1].
  • Here, the functional significance of 28 MBD missense mutations identified in patients were analysed by transient expression of the mutant proteins in cultured cells [10].
  • In these mice the histone deacetylase inhibitor valproate, which increases acetylated histone content in cortical GABAergic interneurons, also prevents MET-induced RELN promoter hypermethylation and reduces the methyl-CpG binding domain protein binding to RELN and GAD67 promoters [11].
  • Despite its negative T15 idiotype profile, N-terminal amino acid sequencing of PCM-1 purified heavy chain and Southern blots of the hybridoma DNA indicated that it utilizes the T15 VH and JH1 genes [12].
  • A synthetic RNA encoding enhanced green fluorescence protein fused to the methyl-CpG-binding domain and nuclear localization signal of human MBD1 was microinjected into metaphase II-arrested or fertilized oocytes, and the localization of methylated DNA was monitored by live cell imaging [13].

Associations of Mbd1 with chemical compounds

  • The mammalian protein MBD4 contains a methyl-CpG binding domain and can enzymatically remove thymine (T) or uracil (U) from a mismatched CpG site in vitro [14].
  • Within the nucleus, FADD interacted with the methyl-CpG binding domain protein 4 (MBD4), which excises thymine from GT mismatches in methylated regions of chromatin [15].
  • 5-halogenated pyrimidine lesions within a CpG sequence context mimic 5-methylcytosine by enhancing the binding of the methyl-CpG-binding domain of methyl-CpG-binding protein 2 (MeCP2) [16].

Regulatory relationships of Mbd1


Other interactions of Mbd1


Analytical, diagnostic and therapeutic context of Mbd1

  • Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2 [19].


  1. Impaired Memory CD8 T Cell Development in the Absence of Methyl-CpG-Binding Domain Protein 2. Kersh, E.N. J. Immunol. (2006) [Pubmed]
  2. Functional analyses of MeCP2 mutations associated with Rett syndrome using transient expression systems. Kudo, S., Nomura, Y., Segawa, M., Fujita, N., Nakao, M., Dragich, J., Schanen, C., Tamura, M. Brain Dev. (2001) [Pubmed]
  3. A component of the transcriptional repressor MeCP1 shares a motif with DNA methyltransferase and HRX proteins. Cross, S.H., Meehan, R.R., Nan, X., Bird, A. Nat. Genet. (1997) [Pubmed]
  4. Gene silencing quantitatively controls the function of a developmental trans-activator. Hutchins, A.S., Mullen, A.C., Lee, H.W., Sykes, K.J., High, F.A., Hendrich, B.D., Bird, A.P., Reiner, S.L. Mol. Cell (2002) [Pubmed]
  5. Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation. Brero, A., Easwaran, H.P., Nowak, D., Grunewald, I., Cremer, T., Leonhardt, H., Cardoso, M.C. J. Cell Biol. (2005) [Pubmed]
  6. Allele-specific histone lysine methylation marks regulatory regions at imprinted mouse genes. Fournier, C., Goto, Y., Ballestar, E., Delaval, K., Hever, A.M., Esteller, M., Feil, R. EMBO J. (2002) [Pubmed]
  7. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Reese, B.E., Bachman, K.E., Baylin, S.B., Rountree, M.R. Mol. Cell. Biol. (2003) [Pubmed]
  8. Mbd1 is recruited to both methylated and nonmethylated CpGs via distinct DNA binding domains. Jørgensen, H.F., Ben-Porath, I., Bird, A.P. Mol. Cell. Biol. (2004) [Pubmed]
  9. The biological functions of the methyl-CpG-binding protein MeCP2 and its implication in Rett syndrome. Nan, X., Bird, A. Brain Dev. (2001) [Pubmed]
  10. Heterogeneity in residual function of MeCP2 carrying missense mutations in the methyl CpG binding domain. Kudo, S., Nomura, Y., Segawa, M., Fujita, N., Nakao, M., Schanen, C., Tamura, M. J. Med. Genet. (2003) [Pubmed]
  11. Reelin and glutamic acid decarboxylase67 promoter remodeling in an epigenetic methionine-induced mouse model of schizophrenia. Dong, E., Agis-Balboa, R.C., Simonini, M.V., Grayson, D.R., Costa, E., Guidotti, A. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  12. Immunologic memory to phosphocholine. IV. Hybridomas representative of Group I (T15-like) and Group II (non-T15-like) antibodies utilize distinct VH genes. Chang, S.P., Perlmutter, R.M., Brown, M., Heusser, C.H., Hood, L., Rittenberg, M.B. J. Immunol. (1984) [Pubmed]
  13. Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging. Yamazaki, T., Yamagata, K., Baba, T. Dev. Biol. (2007) [Pubmed]
  14. Enhanced CpG mutability and tumorigenesis in MBD4-deficient mice. Millar, C.B., Guy, J., Sansom, O.J., Selfridge, J., MacDougall, E., Hendrich, B., Keightley, P.D., Bishop, S.M., Clarke, A.R., Bird, A. Science (2002) [Pubmed]
  15. Fas-associated death domain protein interacts with methyl-CpG binding domain protein 4: a potential link between genome surveillance and apoptosis. Screaton, R.A., Kiessling, S., Sansom, O.J., Millar, C.B., Maddison, K., Bird, A., Clarke, A.R., Frisch, S.M. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  16. 5-halogenated pyrimidine lesions within a CpG sequence context mimic 5-methylcytosine by enhancing the binding of the methyl-CpG-binding domain of methyl-CpG-binding protein 2 (MeCP2). Valinluck, V., Liu, P., Kang, J.I., Burdzy, A., Sowers, L.C. Nucleic Acids Res. (2005) [Pubmed]
  17. Transcriptional regulation of mouse delta-opioid receptor gene by CpG methylation: involvement of Sp3 and a methyl-CpG-binding protein, MBD2, in transcriptional repression of mouse delta-opioid receptor gene in Neuro2A cells. Wang, G., Wei, L.N., Loh, H.H. J. Biol. Chem. (2003) [Pubmed]
  18. DNA methylation-related chromatin modification in the regulation of mouse delta-opioid receptor gene. Wang, G., Liu, T., Wei, L.N., Law, P.Y., Loh, H.H. Mol. Pharmacol. (2005) [Pubmed]
  19. Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2. Nan, X., Meehan, R.R., Bird, A. Nucleic Acids Res. (1993) [Pubmed]
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