The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Rad54l  -  RAD54 like (S. cerevisiae)

Mus musculus

Synonyms: DNA repair and recombination protein RAD54-like, RAD54, RAD54 homolog, Rad54, mHR54, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on Rad54l

  • We have analyzed the phenotype of mouse RAD54-/- (mRAD54-/-) cells [1].
  • Thus, mRAD54 is not required for the recombination processes that generate functional immunoglobulin and T cell receptor genes [1].
  • We show that Lig4 and Rad54 cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations [2].
  • These findings demonstrate a role for NHEJ in the repair of DSBs that occur spontaneously during or after DNA replication, and reveal overlapping functions for NHEJ and Rad54-dependent HR in the repair of such DSBs [2].
  • We have combined mutations in factors from both repair pathways, the HR protein Rad54 and the DNA-end-binding factor Ku80, which has a role in NHEJ [3].

Biological context of Rad54l


Anatomical context of Rad54l

  • These results indicate that mouse B cells must use factors for promoting homologous recombination that are distinct from the Rad54 proteins important in homology-based chicken Ab gene recombinations [6].
  • The level of mHR54 mRNA was elevated in organs of germ cell and lymphoid development and increased mHR54 expression correlated with the meiotic phase of spermatogenesis [7].
  • Wortmannin radiosensitization was observed in Chinese hamster cells (V79-B310H , CHO-K1), mouse mammary carcinoma cells (SR-1), transformed human fibroblast (N2KYSV), chicken B lymphocyte wild-type cells (DT40), and chicken Rad54 knockout cells (Rad54-/-) [8].
  • Cells deleted for Brca2 exon 27 are hypersensitive to gamma-radiation, streptonigrin, mitomycin C and camptothecin and mildly resistant to ICRF-193 which is similar to HR defective cells null for Rad54 [9].

Regulatory relationships of Rad54l

  • We discuss our results with respect to two models that describe how Rad54 stimulates Rad51-mediated DNA strand invasion [10].

Other interactions of Rad54l

  • Two major complementary double-strand break (DSB) repair pathways exist in vertebrates, homologous recombination (HR), which involves Rad54, and non-homologous end-joining, which requires the DNA-dependent protein kinase (DNA-PK) [11].
  • Rad51 forms a nucleoprotein filament on single-stranded DNA (ssDNA) that mediates pairing with and strand invasion of homologous duplex DNA with the assist of Rad54 [10].

Analytical, diagnostic and therapeutic context of Rad54l

  • Gene targeting experiments demonstrate that homologous recombination in mRAD54-/- cells is reduced compared to wild-type cells [1].


  1. Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Essers, J., Hendriks, R.W., Swagemakers, S.M., Troelstra, C., de Wit, J., Bootsma, D., Hoeijmakers, J.H., Kanaar, R. Cell (1997) [Pubmed]
  2. Rad54 and DNA Ligase IV cooperate to maintain mammalian chromatid stability. Mills, K.D., Ferguson, D.O., Essers, J., Eckersdorff, M., Kanaar, R., Alt, F.W. Genes Dev. (2004) [Pubmed]
  3. Collaboration of homologous recombination and nonhomologous end-joining factors for the survival and integrity of mice and cells. Couëdel, C., Mills, K.D., Barchi, M., Shen, L., Olshen, A., Johnson, R.D., Nussenzweig, A., Essers, J., Kanaar, R., Li, G.C., Alt, F.W., Jasin, M. Genes Dev. (2004) [Pubmed]
  4. Role of mammalian Rad54 in telomere length maintenance. Jaco, I., Muñoz, P., Goytisolo, F., Wesoly, J., Bailey, S., Taccioli, G., Blasco, M.A. Mol. Cell. Biol. (2003) [Pubmed]
  5. Mouse RAD54 affects DNA double-strand break repair and sister chromatid exchange. Dronkert, M.L., Beverloo, H.B., Johnson, R.D., Hoeijmakers, J.H., Jasin, M., Kanaar, R. Mol. Cell. Biol. (2000) [Pubmed]
  6. Gene conversion-like sequence transfers between transgenic antibody V genes are independent of RAD54. D'Avirro, N., Truong, D., Luong, M., Kanaar, R., Selsing, E. J. Immunol. (2002) [Pubmed]
  7. Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene: evidence for functional conservation. Kanaar, R., Troelstra, C., Swagemakers, S.M., Essers, J., Smit, B., Franssen, J.H., Pastink, A., Bezzubova, O.Y., Buerstedde, J.M., Clever, B., Heyer, W.D., Hoeijmakers, J.H. Curr. Biol. (1996) [Pubmed]
  8. DNA-PK: the major target for wortmannin-mediated radiosensitization by the inhibition of DSB repair via NHEJ pathway. Hashimoto, M., Rao, S., Tokuno, O., Yamamoto, K., Takata, M., Takeda, S., Utsumi, H. J. Radiat. Res. (2003) [Pubmed]
  9. Embryonic stem cells deficient for Brca2 or Blm exhibit divergent genotoxic profiles that support opposing activities during homologous recombination. Marple, T., Kim, T.M., Hasty, P. Mutat. Res. (2006) [Pubmed]
  10. Analysis of mouse Rad54 expression and its implications for homologous recombination. Essers, J., Hendriks, R.W., Wesoly, J., Beerens, C.E., Smit, B., Hoeijmakers, J.H., Wyman, C., Dronkert, M.L., Kanaar, R. DNA Repair (Amst.) (2002) [Pubmed]
  11. Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair. Fukushima, T., Takata, M., Morrison, C., Araki, R., Fujimori, A., Abe, M., Tatsumi, K., Jasin, M., Dhar, P.K., Sonoda, E., Chiba, T., Takeda, S. J. Biol. Chem. (2001) [Pubmed]
WikiGenes - Universities