Disease relevance of Cd53

• Expression of the gene defined by the pRcT7a cDNA clone in a series of MoMuLV-induced rat T-cell lymphomas showed a perfect correlation with the expression of the rat leukocyte antigen MRC OX-44 [1].

High impact information on Cd53

• To study its biological role, we determined which signaling pathways are affected by the CD53 antigen, a prototypic member of this family, in rat macrophages [2].
• A 50-60-kD glycoprotein coprecipitates with the OX-44 molecule from RNK-16 cells and rat splenic T cells [3].
• The MRC OX-44 molecule, which is expressed on all peripheral leukocytes, identifies the subset of thymocytes capable of proliferating in response to alloantigens and lectins (Paterson, D.J., J.R. Green, W.A. Jefferies, M. Puklavec, and A.F. Williams. 1987. J. Exp. Med. 165:1) [3].
• TCR stimulation resulted in rapid downregulation of CD4 and CD8, maintenance of a larger cell size, and induction of the CD53 antigen that marks mature and CD4,8 double-negative rat thymocytes [4].
• Comparison of NH2-terminal protein sequence from the rat OX-44 antigen with the sequence of the human CD37 antigen deduced from a cDNA clone shows that these antigens are species homologues [5].

Biological context of Cd53

• The pRcT7a protein, which exhibits four putative transmembrane regions and three putative glycosylation sites, contains a region which is nearly identical in sequence to a peptide derived from the rat leukocyte antigen MRC OX-44 [1].
• The CD53 antigen interactions might contribute to cell survival in poorly vascularized regions of the tumor mass [6].
• We have studied the effect of CD53 antigen ligation on the survival response of tumor cells to serum deprivation, a well-known stimulator of cell death that may mimic the tumor environment; for this aim IR938F and Jurkat cells, a B- and T-cell lymphoma, were used [6].
• In addition, ERK1 and ERK2 activation by phosphorylation occurred following CD53 antigen ligation [7].
• Thymocytes of CD4-,CD8+,OX-44- phenotype have been shown to be an intermediate of thymopoiesis that give rise to cells of CD4+, CD8+, OX-44- normal cortical thymocyte phenotype both in vitro and in vivo during thymic regeneration [8].

Anatomical context of Cd53

• Mesangial cell cultures were stimulated with MRC OX-44 antibody [7].
• The OX-44 molecule couples to signaling pathways and is associated with CD2 on rat T lymphocytes and a natural killer cell line [3].
• Magnetic cell sorting (MACS) depletion of fetal liver cells was performed using OX43 and OX44 antibodies [9].
• Multiparametric flow cytometric analysis of the expression of an oval cell antigen, OC.3, which is a bile duct and putative liver stem cell marker, showed that most OC.3-positive cells coexpressed OX-43/OX-44 and morphologically were hemopoietic precursor cells [10].
• Cells were panned first with an antibody to red blood cells to remove erythroid cells and then with monoclonal antibodies OX-43/OX-44 to remove hemopoietic and endothelial cells [10].

Associations of Cd53 with chemical compounds

• Ligation of CD53 antigen with a monoclonal antibody triggered the induction of DNA synthesis, which was not sensitive to inhibitors of signaling pathways that use phosphatidylinositol 3-kinase (PI3K) and protein kinase C, or to calcium channel inhibitors, such as thapsigargin and verapamil [7].
• The epitopes of OX-44, 6E2 and 7D2 are restored by the substitution of a single isoleucine residue for threonine at position 154 in the mouse protein [11].

Other interactions of Cd53

• CD53 antigen and epidermal growth factor induce similar changes in the pattern of phorbol ester binding in a B cell lymphoma [12].

References