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Gene Review

CD53  -  CD53 molecule

Homo sapiens

Synonyms: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25, ...
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Disease relevance of CD53


High impact information on CD53


Biological context of CD53

  • The mouse Cd53 gene spanned approximately 9.0 kb of DNA and encoded the 219 amino acid residues of CD53 over seven exons [8].
  • Gene structure, chromosomal localization, and protein sequence of mouse CD53 (Cd53): evidence that the transmembrane 4 superfamily arose by gene duplication [8].
  • Cross-linking of cell-bound F(ab')2 fragments of two different anti-CD53 mAb with F(ab')2 anti-mouse Ig led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst [9].
  • When aligned with other TM4SF proteins, the deduced amino acid sequence of NAG-2 showed most identity (34%) to CD53 [10].
  • CD53 transcripts increase in prevalence after mitogenic stimulation, suggesting that the protein may be involved in the transport of factors essential for cell proliferation [2].

Anatomical context of CD53


Associations of CD53 with chemical compounds

  • These associations were specific, since we found no evidence for other phosphoinositide kinases (e.g. phosphoinositide 3-kinase and phosphoinositide-4-phosphate 5-kinase) associating with TM4SF proteins, and many other TM4SF proteins (including CD82 and CD53) did not associate with PI 4-kinase [14].
  • This suggests that CD53-mediated signaling is largely independent of protein kinase C. CD53-mediated calcium fluxes were inhibited by high concentrations of staurosporine (300 to 500 nM) but not by ADP-ribosylating toxins, suggesting dependence on tyrosine kinases rather than GTP-binding proteins [9].
  • 7. Comparison of NH2-terminal protein sequence of OX44 rat Ag and CD53 suggest that CD53 is the human homologue of OX44 [2].
  • Cross-linking of CD53 with the use of the mAb MEM-53 and a polyclonal sheep anti-mouse Ig promoted activation of resting B cells into the G1 phase of the cell cycle as judged by increased expression of the early activation Ag CD69, increases in cellular volume, RNA synthesis, and c-myc protein levels, and enhanced binding of 7-aminoactinomycin D [15].
  • Aging of neutrophils in vitro resulted in a significant (P < 0.05) down-regulation of expression of the selectin CD62L, and a significantly increased expression of the two tetraspanins CD53 and CD63 [16].

Regulatory relationships of CD53

  • In contrast, the expression of CD11b was significantly enhanced after 24 h of incubation with anti-CD53 mAb, while the expression of CD62L was significantly reduced with anti-CD81 mAb [17].
  • Cross-linking of anti-CD53 induced negligible S phase entry in the absence of other stimuli [15].

Other interactions of CD53

  • Results suggested that for normal PBL, PMN are distinguished by dominant cytoplasmic CD63; monocytes and B cells prevailingly express CD53; CD82 is primarily expressed on T-cell membranes [18].
  • CD53 and CD37 were not detected on melanoma [19].
  • Taken together, the data suggest that CD53 plays an important functional role in B cell activation and differentiation [15].
  • However, cytokines, in particular IL-2 and IL-4, potentiated the DNA synthesis induced by cross-linking of CD53 [15].
  • Serial frozen sections were immunostained using monoclonal antibodies (MAbs) to monomorphic framework determinants of HLA sublocus products, beta 2m and Ii, and to CD53, a recently defined broadly distributed pan-leucocyte molecule [20].

Analytical, diagnostic and therapeutic context of CD53


  1. Aberrant expression of tetraspanin molecules in B-cell chronic lymphoproliferative disorders and its correlation with normal B-cell maturation. Barrena, S., Almeida, J., Yunta, M., López, A., Fernández-Mosteirín, N., Giralt, M., Romero, M., Perdiguer, L., Delgado, M., Orfao, A., Lazo, P.A. Leukemia (2005) [Pubmed]
  2. Identification and analysis of cDNA clones encoding CD53. A pan-leukocyte antigen related to membrane transport proteins. Amiot, M. J. Immunol. (1990) [Pubmed]
  3. Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activity. Carmo, A.M., Wright, M.D. Eur. J. Immunol. (1995) [Pubmed]
  4. Ligation of CD53/OX44, a tetraspan antigen, induces homotypic adhesion mediated by specific cell-cell interactions. Lazo, P.A., Cuevas, L., Gutierrez del Arroyo, A., Orúe, E. Cell. Immunol. (1997) [Pubmed]
  5. HindIII RFLP in the human CD53 gene on 1p13. Gallego, M.I., Varas, F., Lazo, P.A. Hum. Mol. Genet. (1994) [Pubmed]
  6. Expression of the natural killer cell-associated antigens CD56 and CD57 in human neural and striated muscle cells and in their tumors. Mechtersheimer, G., Staudter, M., Möller, P. Cancer Res. (1991) [Pubmed]
  7. Differential cooperation between regulatory sequences required for human CD53 gene expression. Hernández-Torres, J., Yunta, M., Lazo, P.A. J. Biol. Chem. (2001) [Pubmed]
  8. Gene structure, chromosomal localization, and protein sequence of mouse CD53 (Cd53): evidence that the transmembrane 4 superfamily arose by gene duplication. Wright, M.D., Rochelle, J.M., Tomlinson, M.G., Seldin, M.F., Williams, A.F. Int. Immunol. (1993) [Pubmed]
  9. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. Olweus, J., Lund-Johansen, F., Horejsi, V. J. Immunol. (1993) [Pubmed]
  10. NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that complexes with integrins and other TM4SF proteins. Tachibana, I., Bodorova, J., Berditchevski, F., Zutter, M.M., Hemler, M.E. J. Biol. Chem. (1997) [Pubmed]
  11. Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation. Charrin, S., Manié, S., Oualid, M., Billard, M., Boucheix, C., Rubinstein, E. FEBS Lett. (2002) [Pubmed]
  12. Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes: an association of CD9 with alpha 3 beta 1 integrin. Okochi, H., Kato, M., Nashiro, K., Yoshie, O., Miyazono, K., Furue, M. Br. J. Dermatol. (1997) [Pubmed]
  13. Infection with human T-lymphotropic virus types I and II results in alterations of cellular receptors, including the up-modulation of T-cell counterreceptors CD40, CD54, and CD80 (B7-1). Dezzutti, C.S., Rudolph, D.L., Lal, R.B. Clin. Diagn. Lab. Immunol. (1995) [Pubmed]
  14. Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase. Yauch, R.L., Hemler, M.E. Biochem. J. (2000) [Pubmed]
  15. Cross-linking of CD53 promotes activation of resting human B lymphocytes. Rasmussen, A.M., Blomhoff, H.K., Stokke, T., Horejsi, V., Smeland, E.B. J. Immunol. (1994) [Pubmed]
  16. Increased expression of the tetraspanins CD53 and CD63 on apoptotic human neutrophils. Beinert, T., Münzing, S., Possinger, K., Krombach, F. J. Leukoc. Biol. (2000) [Pubmed]
  17. Functional expression of transmembrane 4 superfamily molecules on human eosinophils. Matsumoto, K., Bochner, B.S., Wakiguchi, H., Kurashige, T. Int. Arch. Allergy Immunol. (1999) [Pubmed]
  18. Expression of tetraspanins in peripheral blood leukocytes: a comparison between normal and infectious conditions. Tohami, T., Drucker, L., Radnay, J., Shapira, H., Lishner, M. Tissue Antigens (2004) [Pubmed]
  19. Expression of the neuroglandular antigen and analogues in melanoma. CD9 expression appears inversely related to metastatic potential of melanoma. Si, Z., Hersey, P. Int. J. Cancer (1993) [Pubmed]
  20. Expression of HLA-A,B,C, beta 2-microglobulin (beta 2m), HLA-DR, -DP, -DQ and of HLA-D-associated invariant chain (Ii) in soft-tissue tumors. Mechtersheimer, G., Staudter, M., Majdic, O., Dörken, B., Moldenhauer, G., Möller, P. Int. J. Cancer (1990) [Pubmed]
  21. Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Yunta, M., Lazo, P.A. Oncogene (2003) [Pubmed]
  22. Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Yunta, M., Rodríguez-Barbero, A., Arévalo, M.A., López-Novoa, J.M., Lazo, P.A. Kidney Int. (2003) [Pubmed]
  23. Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. Mollinedo, F., Martín-Martín, B., Gajate, C., Lazo, P.A. J. Leukoc. Biol. (1998) [Pubmed]
  24. Leukocyte detection in human semen using flow cytometry. Ricci, G., Presani, G., Guaschino, S., Simeone, R., Perticarari, S. Hum. Reprod. (2000) [Pubmed]
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