Disease relevance of IL5

• Average daily gain and days on feed were not affected by treatments, but TRF treatment increased (P less than .05) total intake of dry matter (DM) and feed conversion ratio: 3.00, 3.02, 3.08, and 3.22 kg DM/kg weight gain for control, GRF, TRF, and GTRF, respectively [1].
• Prednisolone and emedastine are effective against IL-5-induced eosinophilia [2].

High impact information on IL5

• TRF analogues without proline or peptide amides without proline are ineffective [3].
• An enzyme which catalyzes the deamidation of thyroliberin (TRF; less than Glu-His-Pro-NH2) has been purified 110-fold from extracts of bovine anterior pituitary by ammonium sulfate fractionation, ion exchange chromatography on DEAE-cellulose, and gel filtration [3].
• Eosinophils were purified from the blood of IL-5 transgenic mice, labeled with (111)In and injected into nontransgenic CBA/Ca mice [4].
• Recent studies from this laboratory have suggested a similarity, if not identity, of thyrotropin releasing factor (TRF) deamidase and post-proline cleaving enzyme [5].
• In addition, both proteins exhibit a molecular weight of 75,000, and have identical Km, values for the synthetic substrate pGlu-(N-benzyl-L-His)-Pro-beta-naphthylamide and identical K1 values for TRF and luteinizing hormone releasing factor as inhibitors [5].

Biological context of IL5

• In animals exposed to 8L:16D, GH levels and AUC after hGRF plus TRF injections reached 177.5 and 180.5 ng/ml, and 2759 and 3704 ng/ml.min at 1000 and 1600 hr (lights on) but only 84.0 and 72.7 ng/ml, and 1544 and 1501 ng/ml.min at 0400 and 2200 hr (lights off), respectively.(ABSTRACT TRUNCATED AT 250 WORDS)[6]
• In the second trial, the effect of GRF (3.3 micrograms.kg-1 BW, sc) and TRF (1.1 micrograms.kg-1 BW, sc) was tested at three stages (18, 72 and 210 days) of lactation on serum Prl and TSH concentrations [7].

Anatomical context of IL5

• Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequence [8].
• Presence of TRF immunoreactivity in marginal islet cells in rat pancreas [9].

Associations of IL5 with chemical compounds

• The response of thyroid-stimulating hormone (TSH) to TRF disappeared (P greater than .10) after 1 mo of treatment, whereas the thyroxine (T4) response decreased (P less than .01) throughout the treatment period [10].
• Prednisolone (3-10 mg/kg, i.p.) and emedastine (30 mg/kg, p.o.) reduced the increased number of eosinophils induced by the IL-5 challenge [2].

Analytical, diagnostic and therapeutic context of IL5

• The present HPLC results suggest that the islets contained TRF [9].
• An intratracheal injection of murine recombinant IL-5 (3-30 microg/animal) induced a dose-dependent increase in the number of eosinophils in the bronchoalveolar lavage fluid of Brown Norway (BN) rats 24 h after administration [2].

References