Disease relevance of IGKV3-15

• We have established a cell line, which we named 380, from a young male with acute lymphoblastic leukemia (FAB type L2) [1].
• A 26-year-old man initially presented with pancytopenia and a bone marrow diffusely replaced with blast cells morphologically most consistent with a French-American-British L2 subclassification [2].
• The specificity of these monoclonal antibodies was determined with a solid-phase immunoassay in which HeLa 229 cells infected with C. trachomatis serovars D, G, H, I, L2 and the Chlamydia psittaci meningopneumonitis strain Cal-10 were used [3].
• Following resolution of genital infection with the mouse pneumonitis (MoPn) biovar of Chlamydia trachomatis, mice were challenged intravaginally with either MoPn or human serovar E or L2 [4].
• Two methods of cell sychronization, density-dependent arrest and double thymidine block, were used to assign two Epstein-Barr virus-associated antigens to different parts of the growth cycle of the human B lymphblastoid cell lines, WI-L2 and Raji [5].

High impact information on IGKV3-15

• L2 protein was found attached to microtubules after uncoating of incoming human papillomavirus pseudovirions [6].
• Mutations within this region abrogating the L2/dynein interaction strongly reduce the infectivity of pseudoviruses, indicating that this interaction mediates the minus-end-directed transport of the viral genome along microtubules towards the nucleus [6].
• Bovine papillomavirus type 1 (BPV-1) late pre-mRNAs are spliced in keratinocytes in a differentiation-specific manner: the late leader 5' splice site alternatively splices to a proximal 3' splice site (at nucleotide 3225) to express L2 or to a distal 3' splice site (at nucleotide 3605) to express L1 [7].
• The floor of the antigen binding cavity is formed mainly by residues from CDR L3 and CDR H3 while a surrounding ridge is formed by residues from all other CDRs except L2 [8].
• An antigen that is dependent on the glycose lacto-N-neotetraose induces IgG in humans that is bactericidal for L2, L3, L4, and L7 strains [9].

Chemical compound and disease context of IGKV3-15

• Monoclonal antibodies were prepared by the fusion of murine myeloma NS1 cells with spleen cells of BALB/c mice immunized with Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar [3].

Biological context of IGKV3-15

• They also had a higher incidence of high white cell count and were more likely to be of the French-American-British (FAB) L2 morphological subtype [10].
• The superinfection showed slow kinetics compared with primary HIV-1 infection of M10 cells, the parent of the L-2 cell clone [11].
• NCAM bears two kinds of functionally important sugar side chains, sialic acid polymers in the fifth domain, which modulate NCAM binding, and the L2 moiety, which is involved in cell adhesion and can be assigned to the third domain [12].
• Like most of the "granular" ALLs described in the literature, the blast cells had L2 morphology and exhibited a common-ALL immunologic phenotype [13].

Anatomical context of IGKV3-15

• We now demonstrate that the L2 protein is able to interact with the microtubule network via the motor protein dynein [6].
• It was of the same size as Fc epsilon RII/CD23 mRNA of the lymphoblastoid B cell line WI-L2 [14].
• However, after isopycnic centrifugation of crude lysosomal fractions in Percoll gradients, 125I in old thyroids was not distributed mainly in the dense fraction L1 (lysosomes) as in young thyroids, but partly in particles of lower density (light L2 and buoyant fractions) [15].
• RESULTS: All ALL cases (L1 and L2) were TdT-positive, except for 3 cases that were of early T-cell lineage [16].
• L2/HNK-1, which is found on motor axon-associated Schwann cells, may provide regenerating motor axons with a selective advantage over others resulting in appropriate reinnervation of motor pathways [17].

Analytical, diagnostic and therapeutic context of IGKV3-15

• Based on immunofluorescence and coimmunoprecipitation analyses, the L2 region interacting with dynein is mapped to the C-terminal 40 amino acids [6].
• We evaluated a simple enzyme-linked immunosorbent assay in which serotype L2 elementary bodies are used as antigen [18].
• An improved solid-phase enzyme immunoassay (EIA) with Chlamydia trachomatis L2 434/Bu elementary bodies was developed for the measurement of immunoglobulin M (IgM) antibody to C. trachomatis in serum [19].
• Chlamydial IgG antibodies, as determined by the indirect immunofluorescence test with inclusions of C. trachomatis L2 as antigens, persisted at stable levels in 43% of the women for up to 6 years; 43% of the women showed a decrease in IgG titer, and 13% showed an increase [20].
• Comparative flow cytometry of L-2 and M10 cells revealed that the cell surface of L-2 cells despite expressing HIV-1 env protein, reacted slightly with OKT4 or anti-CD4(68-130) MAb, but not with Leu-3a or OKT4A MAb [11].

References