Disease relevance of Centrifugation, Isopycnic

• The metabolism and disposition of 5-propyl-2'-deoxyuridine (Pr-dUrd) in herpes simplex virus type 1 infections were investigated in cell culture using [14C]Pr-dUrd, [32P]orthophosphate, and several methods including high pressure liquid chromatography and isopycnic centrifugation [1].
• Mercurated DNA was synthesized in permeable cells of Bacillus subtilis, using 5-mercurideoxycytidine triphosphate as one of the substrates, and was separated from parental unsubstituted DNA by isopycnic centrifugation in CsCl gradients [2].
• DNA-protein complexes derived from a recombinant adenovirus (AVC2.null) were isolated by sequential use of CsCl step gradients followed by isopycnic centrifugation in a mixture of CsCl and guanidine HCl [3].
• Infection of 5-iodo-2'-deoxyuridine (IUdR)-arrested cells with either of the simian cytomegaloviruses, however, induced synthesis of cellular DNA of normal density; synthesis of cellular DNA substituted with IUdR as evidenced by resolution of a heavy DNA peak after isopycnic centrifugation was not observed [4].
• The 40- to 50-nm pleomorphic particles found in the sera of domestic Pekin ducks infected with duck hepatitis B virus were purified by rate zonal and isopycnic centrifugation [5].

High impact information on Centrifugation, Isopycnic

• It enabled us to establish the density distribution after isopycnic centrifugation in a linear gradient of sucrose [6].
• Isopycnic centrifugation in cesium chloride removed the remaining outer membrane protein contaminants [7].
• The location of the ligand, generally asialofetuin, was analyzed by isopycnic centrifugation on Nycodenz gradients [8].
• 60: 604-617), was subfractionated by isopycnic centrifugation in a metrizamide gradient and the distribution of several marker enzymes was established [9].
• To more precisely localize these proteins to the RER, native RMs and RMs stripped of ribosomes by puromycin/high salt were subjected to isopycnic centrifugation [10].

Chemical compound and disease context of Centrifugation, Isopycnic

• Inner and outer membranes of Escherichia coli were separated by isopycnic centrifugation in sucrose gradients and analyzed for the presence of penicillin-binding proteins [11].
• 6. Only those fractions containing double-shelled rotavirus particles isolated by isopycnic centrifugation in cesium chloride had hemagglutinating activity [12].
• The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted [13].
• Infectious bovine rhinotracheitis (IBR) virus was purified by rate zonal and isopycnic centrifugation in potassium tartrate gradients [14].
• The buoyant densities of Escherichia coli K12 were investigated by isopycnic centrifugation in gradients of colloidal silica (Ludox) and polyvinylpyrrolidone [15].

Biological context of Centrifugation, Isopycnic

• DNA was isolated and purified from such chloroplasts, and characterized with respect to cesium chloride density gradient isopycnic centrifugation, denaturation, renaturation kinetics and restriction enzyme analysis [16].

Anatomical context of Centrifugation, Isopycnic

• Isolation of rat liver lysosomes by isopycnic centrifugation in a metrizamide gradient [9].
• Plasma membranes were obtained by rate centrifugation followed by isopycnic centrifugation on a sucrose gradient [17].
• Differential and isopycnic centrifugation of rat liver homogenates showed that, besides its established localization in peroxisomes and endoplasmic reticulum, dihydroxyacetone-phosphate acyltransferase is also present in mitochondria [18].
• 2. Isopycnic centrifugation in sucrose gradients of a whole liver homogenate showed that the ligand is sequentially associated with three organelles with increasing buoyant densities [19].
• Rat skeletal muscle membranes were prepared by isopycnic centrifugation in sucrose gradients [20].

Associations of Centrifugation, Isopycnic with chemical compounds

• By this assay, the vesicles, detected by their acetylcholine (ACh) content and the antigens detected by the RIA, have the same buoyant density after isopycnic centrifugation of crude membrane fractions on sucrose and glycerol density gradients [21].
• This autonomous low-molecular-weight DNA species could be specifically amplified by cycloheximide treatment and was shown by isopycnic centrifugation in cesium chloride-ethidium bromide to consist predominantly of covalently closed circular DNA molecules [22].
• Further purification and fractionation of proteoglycans was achieved by a second CsCl isopycnic centrifugation (rho 0 = 1.45) in 4.0 M guanidinium chloride [23].
• 5'-Nucleotidase, alkaline phosphodiesterase, gamma-glutamyltranspeptidase, and cholesterol were membrane-bound and distributed identically after isopycnic centrifugation with peaks at 1.15 [24].
• The purified glycoprotein had a high density (1.40 g/ml) on isopycnic centrifugation indicating a high carbohydrate content [25].

Gene context of Centrifugation, Isopycnic

• Dipeptidyl aminopeptidase yscIV, the STE13 gene product, was found to be associated with plasma membrane vesicles after sucrose gradient isopycnic centrifugation [26].
• Consistent with this conclusion was our finding that chemical iodination, as well as enzymatic iodination, led to formation of uniformly iodinated Tg, as determined by isopycnic centrifugation in rubidium chloride [27].
• The following hormone granules (equilibrium densities between parentheses) were separated by isopycnic centrifugation on the sucrose density gradients: VIP (1 x 17), motilin (1 x 20), and secretin (1 x 24) [28].
• In this study, we investigated the cellular localization of the HrcC and HrcJ proteins by Triton solubilization, sucrose-gradient isopycnic centrifugation, and immunogold labeling of the bacterial cell surface [29].
• We isolated and characterized heparan sulfate rich proteoglycan (HSPG) present in the basement membrane from 7 M urea insoluble materials of the human placenta by CsCl isopycnic centrifugation, anion-exchange chromatography and gel filtration [30].

Analytical, diagnostic and therapeutic context of Centrifugation, Isopycnic

• The resulting protein-detergent complex was characterized by gel filtration on Sepharose 6B, sedimentation rate determination in linear sucrose gradients, and equilibrium isopycnic centrifugation in NaBr and CsCl [31].
• The activities of N-acetylglutamate synthase and the first two urea cycle enzymes, carbamoylphosphate synthase (ammonia) and ornithine carbamoyltransferase [EC 2.1.3.3], were cofractionated with mitochondrial marker enzymes during the cell fractionation and the isopycnic centrifugation [32].
• The method involves the following steps: precipitation of serum with polyethylene glycol 6000, affinity chromatography on concanavalin A-Sepharose and on omega-aminononyl-Sepharose, and isopycnic centrifugation [33].
• The viruses were separated by isopycnic centrifugation in "self formed" caesium chloride gradients, using a Beckman Model E analytical centrifuge in which a separation cell fitted with a centerpiece with two perforated partitions was used [34].
• Further analysis by electron microscopy and caesium chloride isopycnic centrifugation confirmed the presence of picobirnaviruses in the samples [35].

References