Disease relevance of LOC416236

• A 15-kilobase pair EcoRI chick DNA fragment, containing both the termination codon UGA and the 5'-portion of the structural ovomucoid gene, has been cloned in lambda phage Charon 4A by in vitro packaging [1].
• BACKGROUND: Chicken ovomucoid (OM, Gal d 1) has an important role in the pathogenesis of IgE-mediated allergic reactions to hen's egg white [2].

High impact information on LOC416236

• Finally, the positions of the introns within the ovomucoid gene support the theory that introns separate gene segments that code for functional domains of proteins and provide insight on the manner by which eucaryotic genes were constructed during the process of evolution [3].
• Processing of high molecular weight ovalbumin and ovomucoid precursor RNAs to messenger RNA [4].
• Two thirds of the natural chicken ovomucoid gene has been sequenced, including all exons and the intron sequences surrounding all fourteen intron/exon junctions [3].
• Identification of potential ovomucoid mRNA precursors in chick oviduct nuclei [5].
• The nucleic acid sequence agrees with the known amino acid sequences for both ovomucoid and its N-terminal signal peptide [6].

Biological context of LOC416236

• The longest plasmid containing ovomucoid DNA sequences was designated pOM502 [6].
• Chicken ovomucoid: determination of its amino acid sequence, determination of the trypsin reactive site, and preparation of all three of its domains [7].
• Linkage mapping of chicken ovoinhibitor and ovomucoid genes to chromosome 13 [8].
• The results indicate that the seven intervening sequence regions of the ovomucoid precursor are removed in a preferred but not necessarily an obligatory order [4].
• Maturation had no detectable effect on the glycosylation of ovalbumin, whereas it affected the glycosylation of chick ovomucoid [9].

Anatomical context of LOC416236

• Ovomucoid messenger RNA (mRNAom) comprises approximately 8% of the total mRNA in the estrogen-stimulated oviduct [6].
• The translation of ovomucoid mRNA in a reticulocyte lysate protein-synthesizing system yields a precursor form which contains an NH2-terminal extension of 23 amino acid residues [10].
• As with the dog membranes, the precursor form of human placental lactogen is transported into the vesicles and processed to the native secretory form by an associated "signal peptidase." The oviduct microsomal membranes glycosylate nascent ovomucoid and ovalbumin in vitro [11].
• The peptides, together with intact, denatured and oxidized OM, were used to stimulate patient-derived cell cultures for mapping T cell epitopes [2].
• CONCLUSIONS: One novel B cell epitope (OM 61-74) and 10 T cell epitopes have been identified [2].

Associations of LOC416236 with chemical compounds

• The first and third domains of OMCHI are relatively ineffective inhibitors of several serine proteinases against which they were tested [7].
• The results showed that 60 mg chicken ovomucoid could be immobilized on l g chitosan, and the maximum binding capacity for trypsin was about 8.10(4) U/g dry adsorbent [12].
• Ovomucoid (Gal d1) is a major allergen in hen egg white, consisting of three tandem domains [13].
• The affinity ligand, chicken ovomucoid, was immobilized on the chitosan via a cross-linker, glutaraldehyde [12].
• The present study indicated that glycine 32 and phenylalanine 37 have an important role on its antigenicity and allergenicity as well as structural integrity of ovomucoid DIII [13].

Other interactions of LOC416236

• Separation of enantiomers on a chiral stationary phase based on ovoglycoprotein. II. Comparison of chiral recognition properties with crude ovomucoid [14].

Analytical, diagnostic and therapeutic context of LOC416236

• Recombinant plasmids containing ovomucoid DNA sequences were selected by in situ hybridization to 32P-labeled pOM100 fragments [6].
• Furthermore, sequence analyses suggest that introns were present in the primordial ovomucoid gene before birds and mammals diverged, about 300 million years ago [3].
• Restriction mapping and electron microscopic analyses of this cloned DNA have revealed that the structural ovomucoid gene sequences are separated by at least six intervening sequences [1].
• Separation of sialyl-oligosaccharides by high-performance liquid chromatography. Application to analysis of carbohydrate units of acidic oligosaccharides obtained by hydrazinolysis of hen ovomucoid [15].
• Furthermore, the HPLC profile of duck ovomucoid oligosaccharides was compared with previously reported profiles obtained from quail and chicken ovomucoids [16].

References