High impact information on G6PD

• 2. In contrast with their effects on pyruvate kinase, insulin and dexamethasone acted synergistically to increase the activity of glucose-6-phosphate dehydrogenase of sheep adipose tissue maintained in culture [1].
• These differences were matched by a lower activity of glucose 6-phosphate dehydrogenase and a higher pyruvate dehydrogenase activity in fat-cells from the foetal lambs [2].
• Glutamate and malate dehydrogenases are present, as are glucose-6-phosphate dehydrogenase activity (65.7 nmol mg of protein-1 min-1) and 6-phosphogluconate dehydrogenase activity (63.0 nmol mg of protein-1 min-1).(ABSTRACT TRUNCATED AT 250 WORDS)[3]
• The sequential changes in several glycolytic enzymes, glucose-6-phosphate dehydrogenase, glycolytic intermediates, and adenosine triphosphate, as well as intracellular pH and plasma inorganic phosphorus were followed simultaneously in eight lambs from birth to 2 months of age [4].
• O. venulosum was isolated from sheep and goat; G6PD and MDH isoenzymatic patterns discriminated this species from porcine species of Oesophagostomum [5].

Biological context of G6PD

• Given the fact that TCA and DCA are now being found in community drinking water supplies at levels greater than 100 micrograms/liter, there is a need to further investigate their effects on biological systems, including those with a compromised ability to deal with oxidant stress (e.g., G-6-PD-deficient erythrocytes) [6].
• No significant changes were seen in the growth rates (90 days exposure), in the plasma enzymes alkaline phosphatase, glutamic oxaloacetic transaminase, glucose 6-phosphate dehydrogenase or succinic dehydrogenase, or in the hematocrit or plasma protein concentrations after either the 90-day or 19-day exposures [7].
• 4. The diet-induced differences in lipogenesis were reflected in significant increases in the specific activities of the fatty acid synthetase system, glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase in concentrate fed animals [8].
• Purification and kinetics of sheep kidney cortex glucose-6-phosphate dehydrogenase [9].
• The present study reveals that irrespective of origin, GH in vitro has a direct inhibitory effect on lipogenic enzymes ME, G6PDH, ICDH and a stimulatory effect on transaminases AST and ALT in A. testudineus, thus favoring gluconeogenesis [10].

Anatomical context of G6PD

• In addition, in vitro effects of gentamicin sulphate, penicillin G potassium, amicasin on sheep red blood cell G6PD enzyme activity were investigated [11].
• We have investigated some properties and kinetic mechanism of the sheep kidney cortex G-6-PD [9].

Associations of G6PD with chemical compounds

• CONCLUSIONS: If a patient with G6PD deficiency requires gentamicin sulfate or vancomycin hydrochloride, routine ophthalmic did not inhibit this enzyme [12].
• Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) was purified from sheep erythrocytes, using a simple and rapid method [11].
• METHODS: G6PD was purified from sheep lenses with a yield of 66.8% and a specific activity of 7.8 U/mg proteins, and 10,400-fold using ammonium sulfate fractionation and 2',5'-ADP Sepharose 4B affinity gel [12].
• Erythrocytes of Dorset sheep, an animal model with an erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) deficiency, responded in a dose-dependent manner to the oxidant stress of di- and trichloroacetic acids (DCA and TCA) as measured by increases in methemoglobin (METHB) and decreases in glutathione (GSH) [6].
• The effects of di- and trichloroacetic acid on sheep erythrocytes: an animal model with a glucose-6-phosphate dehydrogenase deficiency [6].

Other interactions of G6PD

• Postmortem longissimus muscle, s.c. fat, intermuscular fat (INT), and kidney fat (KP) samples were assayed in vitro for glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activity (nanomoles.minute-1.gram of tissue-1), and samples were subjected to electrophoresis (PAGE) to separate tissue-specific isoforms [13].
• 2. These comparisons indicate that the erythrocyte enzymes which show significant changes with cell age are glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphoglucose isomerase, lactate dehydrogenase, glutathione reductase, enolase and phosphoglycerate kinase [14].

Analytical, diagnostic and therapeutic context of G6PD

• The effects of intramuscular injection of synthetic racemic equol (+/- 3-(4-hydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-7-ol) into wethers have been examined with respect to maintenance of plasma level, teat growth rate and the activity of the respiratory enzyme glucose-6-phosphate dehydrogenase [15].

References