Disease relevance of Fer1HCH

• Synthesis of ferritin HCH subunit mRNAs that lack an IRE may be important under conditions of iron overload [1].
• Changes in toxicity of the individual isomer solutions were considered to be due, in part, to interconversions of HCH isomers [2].

High impact information on Fer1HCH

• This ferritin contains a putative mitochondrial targeting sequence and an epitope-tagged version localizes to mitochondria in transfected cells [3].
• In parallel with the differential larval and adult phenotypes, our results indicate that dfh silencing differentially dysregulates ferritin expression in adults but not in larvae [4].
• Proteins whose levels increase after clotting include a ferritin-subunit and two members of the immunoglobulin family with a high similarity to the small immunoglobulin-like molecules involved in mammalian innate immunity [5].
• Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron-responsive element [6].
• The deduced amino acid sequence of the D. melanogaster ferritin subunit contained a signal sequence and resembled most closely ferritin of the mosquito Aedes aegypti [7].

Biological context of Fer1HCH

• These genes, located at position 99F1, have different exon-intron structures (Fer1HCH has three introns and Fer2LCH has two introns) and are divergently transcribed [8].
• The structure of two other invertebrate ferritin genes, from the nematode Caenorhabditis elegans (located on chromosomes I and V), was also analyzed [8].
• When Northern blots were probed with a HCH cDNA, two bands were observed, representing at least two messages of different sizes that result from the choice of two different polyadenylation sites [9].
• Conserved ferritin PCR primers were used to amplify a 360-bp cDNA product, which was used to isolate a clone from a D. melanogaster cDNA library that contained the complete coding sequence for a ferritin subunit [7].
• Dynamic acylation of ferritin may slow but not prevent its passage out of the ER [10].

Anatomical context of Fer1HCH

• At pupariation, ferritin becomes more abundant in hemolymph than in other tissues [11].
• Northern blot analysis indicated that there were 1.5- and 1.75-fold increases in the expression of ferritin mRNA after iron-fed fat body and midgut, respectively [12].
• Also, we confirmed that the ferritin mRNA is not expressed in adult ovary and testis [12].

Associations of Fer1HCH with chemical compounds

• On polyacrylamide gel electrophoresis, the product showed two protein bands corresponding to the ferritin monomer and dimer [7].
• Insect messenger RNAs for ferritin and succinate dehydrogenase subunit b have IREs that are active translational control sites [13].

Other interactions of Fer1HCH

• Western blotting reveals that LCH is the predominant ferritin subunit in all life stages [11].

Analytical, diagnostic and therapeutic context of Fer1HCH

• Ferritin was purified from iron-fed Drosophila melanogaster extracts by centrifugation in a gradient of potassium bromide [7].
• The PCR product was also used to locate the position of the ferritin subunit gene at region 99F on the right arm of the third chromosome [7].
• The ferritin 26 kDa subunit cDNA was obtained from RT-PCR using primer designed from N-terminal sequence analysis [12].
• Secreted ferritin subunits are of two kinds in insects molecular cloning of cDNAs encoding two major subunits of secreted ferritin from Calpodes ethlius [10].

References