Disease relevance of IL12A

• These results suggest that the inhibitory effect of IL-12 on both virus replication and apoptosis has potential implications for the design of immunotherapy strategies using IL-12 in FIV infection [1].
• Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies [2].
• In contrast, three of five kittens given FeLV DNA and IL-12 became persistently viraemic [3].
• This adenoviral vector will be a useful tool to examine the effects of intra-tumoural IL-12 delivery in a spontaneously occurring feline soft tissue sarcoma model [4].
• The interleukin-12 gene has been placed under control of a heat inducible promoter, human heat shock promoter 70b, with the intent of spatially and temporally controlling the expression of IL-12, thus limiting its toxicity [4].

High impact information on IL12A

• Interleukin-12, dendritic cells, and the initiation of host-protective mechanisms against Toxoplasma gondii [5].
• Protection correlated weakly with cytotoxic T-lymphocyte activity and increased cytokine transcription of IL-12, gamma interferon, and IL-10 by peripheral blood mononuclear cells in the postchallenge period [6].
• Immunization of cats against feline immunodeficiency virus (FIV) infection by using minimalistic immunogenic defined gene expression vector vaccines expressing FIV gp140 alone or with feline interleukin-12 (IL-12), IL-16, or a CpG motif [6].
• Four groups of cats, each containing four animals, were immunized at 0, 3, and 6 weeks with minimalistic immunogenic defined gene expression vector (MIDGE) vaccines containing the gene(s) for feline immunodeficiency virus (FIV) gp140, FIV gp140 and feline interleukin-12 (IL-12), FIV gp140 and feline IL-16, or FIV gp140 and a CpG motif [6].
• IL-12 and IL-18 constructs were modified to ensure effective expression, and bioactivity was demonstrated using specific assays [3].

Biological context of IL12A

• Addition of human recombinant IL-12 significantly inhibited the virus replication and apoptosis in Fel-039 cells in a dose dependent manner [1].
• Interleukin-12 (IL-12) is an important cytokine for Th1 response which stimulates the T-cell population to produce cytokines for cellular immunity [1].
• To test the ability of these multi-gene vaccines to protect against two feline immunodeficiency virus (FIV) isolates of differing virulence, cats were immunized using either DNA vaccine alone or co-administered with interleukin-12 (IL-12) and/or interleukin-18 (IL-18) cytokine DNA [7].
• In vitro, the transfection efficiency of the adenoviral vector, the effect of multiplicity of infection and the production of biologically active feline IL-12 were studied in the infected cells in response to a range of temperatures [4].
• The maximum tolerated dose of the feline IL-12 adenoviral vector was 10(10) pfu/tumor as dose-limiting toxicities were noted at the 4 x 10(10) pfu dose [8].

Anatomical context of IL12A

• Interleukin-12, Interferon-gamma and Interleukin-4 mRNA levels in cells of the spleen and mesenteric lymph nodes of cats following primary and secondary infection with Toxoplasma gondii were examined by a semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) method [9].

Other interactions of IL12A

• This study extends the data on IL-12 and provides new results on CpG motifs and IL-16 used as adjuvants in the FIV cat model [6].

Analytical, diagnostic and therapeutic context of IL12A

• Molecular cloning of cat interleukin-12 [10].
• Induction of interleukin-12 p40 transcript by CD40 ligation via activation of nuclear factor-kappaB [11].
• Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy [4].
• To achieve localized intratumoral gene expression, an adenoviral gene therapy vector with IL-12 controlled by a heat-inducible promoter (heat shock promoter 70B) was developed and tested in a phase I clinical trial in cats with spontaneously arising soft tissue sarcoma [8].

References