Disease relevance of Poll

• Disorder of the inhibitory glycine receptor: inherited myoclonus in Poll Hereford calves [1].
• Inherited congenital myoclonus in Poll Hereford calves is characterized by hyperesthesia and myoclonic jerks of the skeletal musculature that occur spontaneously and in response to sensory stimuli [1].
• Pol lambda(-/-) mice also show situs inversus totalis and chronic suppurative sinusitis [2].

High impact information on Poll

• The Y-family DNA polymerase kappa (pol kappa) functions in mammalian nucleotide-excision repair [3].
• DNA polymerase kappa (pol kappa) is a member of the Y-family of DNA polymerases that are thought to function in translesion synthesis (TLS) past different types of DNA damage [3].
• Pol lambda(-/-) mice develop hydrocephalus with marked dilation of the lateral ventricles and exhibit a high rate of mortality after birth, although embryonic development appears normal [2].
• Microinjection of sperm from male Pol lambda(-/-) mice into oocytes gives rise to normal offspring, suggesting that the meiotic process is not impaired [2].
• RNA interference-mediated pol-beta suppression was sufficient to increase temozolomide efficacy, whereas a deficiency in pol-iota or pol-lambda did not increase temozolomide-mediated cytotoxicity [4].

Biological context of Poll

• Recently, deletion of DNA polymerase lambda (Poll) in mice produced a phenotype characteristic of PCD (Kobayashi et al., 2002, Mol. Cell. Biol. 22:2769-2776) [5].
• Sensitivity of Pol lambda(-/-) cells to various kinds of DNA damage is indistinguishable from that of Pol lambda(+/+) cells [2].
• DNA polymerase lambda mediates a back-up base excision repair activity in extracts of mouse embryonic fibroblasts [6].
• These first evidences, although indirect, suggest a potential role of Pol lambda in DNA repair synthesis associated with meiosis [7].
• A new gene (POLL) encoding a novel DNA polymerase (Pol lambda) has been identified at mouse chromosome 19 [7].

Anatomical context of Poll

• These data indicate that pol lambda is able to contribute to BER in mouse fibroblast cell extract [6].
• Northern blotting, in situ hybridization analysis and immunostaining showed high levels of Pol lambda specifically expressed in testis, being developmentally regulated and mainly associated to pachytene spermatocytes [7].
• Murine Pol lambda, consisting of 573 amino acid residues, has a 32% identity to Pol beta, involved in nuclear DNA repair in eukaryotic cells [7].
• The present studies demonstrate that the specialized DNA polymerase kappa (polkappa) is uniquely and preferentially expressed in the adrenal cortex and testis of the mouse, as well as in a variety of other tissues [8].

Associations of Poll with chemical compounds

• Pol lambda also conserves the critical residues of Pol beta required for its intrinsic deoxyribose phosphate lyase (dRPase) activity [7].
• We found here that another phenolic compound, curcumin (diferuloylmethane), which is known as an anti-chronic inflammatory agent and is structurally quite similar to petasiphenol, was also a potent pol lambda inhibitor [9].

References