Disease relevance of SLC12A9

• The p21WAF1/Cip1 (p21) protein, a negative regulator of G1 checkpoint control, was overexpressed in the majority of human gliomas [1].
• p21/waf1/cip1 in gastric cancer: associations with histopathological subtypes, lymphonodal metastasis, prognosis and p53 status [2].
• Correlation between TGF-beta1 and p21 (WAF1/CIP1) expression and prognosis in resectable invasive ductal carcinoma of the pancreas [3].
• The antisense efficiency of LNA-containing oligonucleotides was systematically compared with standard PS AONs targeting expression of two endogenous proteins in the human breast cancer cell line MCF-7, namely, the cyclin-dependent kinase inhibitor p21(WAF1/CIP1) and the estrogen receptor alpha (ERalpha) [4].
• The p21WAF1/CIP1 gene mRNA and P21 protein of 45 cases of osteosarcoma were investigated by using in situ hybridization and immunohistochemistry, respectively [5].

High impact information on SLC12A9

• Among them, only up-regulation of manganese superoxide dismutase and the cyclin-dependent kinase inhibitor p21(Waf1/Cip1), proteins known to be redox sensitive and to have cell cycle regulatory functions, correlated with cell growth inhibition [6].
• CIP1 may be part of a novel family of proteins that modifies the activity or kinetics of CCCs through heterodimer formation [7].
• KLF2 inhibits Jurkat T leukemia cell growth via upregulation of cyclin-dependent kinase inhibitor p21WAF1/CIP1 [8].
• DNA damage and p21(WAF1/CIP1/SDI1) in experimental injury of the rat adrenal cortex and trauma-associated damage of the human adrenal cortex [9].
• Age-related differences in the relative expression of p16(INK4a) and p21(WAF1/Cip1) and in the kinetics of Rb hyperphosphorylation led to the conclusion that, in addition to the normal inhibitory activity of p27(Kip1), there is an age-dependent increase in negative regulation of the cell cycle by p16(INK4a) and p21(WAF1/Cip1) [10].

Biological context of SLC12A9

• In conclusion, ATP, in part, blocked H(2)O(2)-induced increase of p21(WAF1/Cip1) and p27(Kip1) expression through PI3K and Akt signal pathway in renal PTCs [11].
• Nuclear run-on transcription assays and p21 mRNA half-life studies indicated that iron was required to support transcriptional activation of p21(WAF1/CIP1) after a PMA stimulus [12].
• Urothelial cells treated with PPARgamma ligands showed drastic morphologic changes and cell cycle arrest at G0/G1 phase accompanied with increased mRNA level of a cyclin-dependent kinase inhibitor p21(WAF1/CIP1) [13].

Anatomical context of SLC12A9

• Of those CKIs, p16(INK4a), p21(WAF1/Cip1), and p27(Kip1) are expressed in corneal endothelium [10].

Associations of SLC12A9 with chemical compounds

• In contrast, ATP increased the level of thymidine, BrdU incorporation (>10(-5) M), and decreased the p21(WAF/Cip1) and p27(Kip1) expression levels, suggesting that ATP has a protective effect against H(2)O(2)-induced oxidative damage [11].
• Extracellular adenosine triphosphate protects oxidative stress-induced increase of p21(WAF1/Cip1) and p27(Kip1) expression in primary cultured renal proximal tubule cells: Role of PI3K and Akt signaling [11].
• However, co-transfection of HSV-tk with Cip1/Waf1 encoding the p21(cip1/waf1) inhibitor of cdks, allows 100 microM ganciclovir to eradicate the population of tumor cells [14].

Other interactions of SLC12A9

• The transported substrates of the remaining two SLC12 family members, SLC12A8 and SLC12A9, are as yet unknown [15].

Analytical, diagnostic and therapeutic context of SLC12A9

• After the cells reached confluence, total protein was extracted, and the relative expression of p16(INK4a), p21(WAF1/Cip1), and p27(Kip1) was determined by Western blot analysis [10].
• RESULTS: Immunocytochemistry confirmed the expression and nuclear localization of p16(INK4a), p21(WAF1/Cip1), and p27(Kip1) in HCECs in situ [10].

References