Disease relevance of CA11

• Steady-state chemostat cultures of Azotobacter vinelandii strain CA11, carrying a deletion of genes encoding the structural polypeptides of nitrogenase nifHDK, were established in a simple defined medium chemically purified to minimize contamination by Mo [1].
• Human stomach-specific gene, CA11, is down-regulated in gastric cancer [2].
• A hemolysin produced by Aeromonas hydrophila CA-11, isolated from an environmental source, was purified by sulfopropyl-Sephadex C-25 chromatography at pH 5 [3].

High impact information on CA11

• CA-RP XI was expressed in the neural cell body, neurites, and astrocytes in relatively limited regions of the brain [4].
• The human CA11 gene appears to be located between the secretor type alpha(1,2)-fucosyltransferase gene cluster (FUT1-FUT2-FUT2P) and the D-site binding protein gene (DBP) on chromosome 19q13 [5].
• In polyacrylamide gel electrophoreses at pHs 4.0 and 9.4 and in thin-layer isoelectric focusing, CA-11 hemolysin migrated as a single band to a position different from that of AH-1 hemolysin [3].
• Immunohistochemical analysis with anti CA11 antibody showed that CA11-positive staining was observed in the surface regions of normal gastric epithelium, but was found faintly or not at all in cancer tissues [2].
• Northern blot analysis of 23 human tissues indicates that CARP2 is expressed abundantly in the brain with moderate expression also present in spinal cord and thyroid [6].

Biological context of CA11

• A single copy of the CA11 gene was localized to the human chromosome 19q13.2-3 [7].
• The deduced amino acid sequence showed an overall similarity of 25-57% to other CA isozymes and the highest % similarity to a CA-RP XI [8].
• Ectopic expression of CA-RP XI in GIST-T1 cells induced cell proliferation and invasion in vitro [9].
• The deduced amino acid sequence of CA-RP XI showed an overall similarity of 42-53% to the active site residues of other active CA isozymes; however, it lacked three zinc-binding histidine residues, raising questions regarding its CA catalytic activity [7].

Anatomical context of CA11

• These results suggest that CA-RP XI plays a general role in the human central nervous system [7].

Associations of CA11 with chemical compounds

• Similar to CA-RP XI, CA-RP X lacked two out of three zinc-liganded histidine residues, suggesting no biological activity of CA [8].

Other interactions of CA11

• A full-length cDNA clone of a human carbonic anhydrase-related protein, CA-RP XI encoded by CA11, was obtained and sequenced [7].

Analytical, diagnostic and therapeutic context of CA11

• Neutralization tests with antihemolysin sera also demonstrated immunological cross-reactivity between AH-1 and CA-11 hemolysins [3].

References