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MeSH Review

Neutralization Tests

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Disease relevance of Neutralization Tests


High impact information on Neutralization Tests

  • Additional significant biological activity for the anti-47 K mol wt immunogen mAb was revealed through results of the in vitro-in vivo neutralization test of Bishop and Miller, in which a 99% or 100% neutralizing activity was demonstrated [6].
  • Neutralization tests of this factor-induced proliferation of spleen cells revealed that a major part of the factor may be GM-CSF or a molecule closely related to it [7].
  • Tissue culture neutralization tests were performed on the sera from 9 patients; significant rises in the antibody titres (greater than or equal to 1:16) to AHC virus antigens were found in 8 cases, and in 2 of them a fourfold rise in the paired sera was noted [8].
  • A comparison of mean antibody titers of post-inoculation sera showed that the RIA was 300 and 1000 times more sensitive than the HI and neutralization tests, respectively [9].
  • Both Fab 5H2 and Fab 5D9 neutralized DENV-4 efficiently with a titer of 0.24 to 0.58 micro g/ml by plaque reduction neutralization test (PRNT), whereas DENV-4-neutralizing activity of other Fabs was low or not detected [10].

Chemical compound and disease context of Neutralization Tests


Biological context of Neutralization Tests


Anatomical context of Neutralization Tests

  • In vitro neutralization tests with pure antibody and IL-6 demonstrated that, with both antibodies tested, more than 1000-fold molar excess of antibody is needed for neutralization in the hybridoma growth assay [18].
  • In the Winn tumor neutralization test, peritoneal macrophages harvested from normal mice 24 h after a single injection of liposomes (2.5 mumol) containing LYNK or CRP markedly inhibited tumor growth when a mixture of effector-target cells (40 : 1) was injected in the footpad [19].
  • The sera were screened for neutralizing antibody to Lumbo (LUM), snowshoe hare (SSH), and trivittatus (TVT) viruses by plaque neutralization tests on Vero cells [20].
  • The sera with rheumatoid factor (RF) that did not agglutinate human Rh+ erythrocytes sensitized by incomplete Rh antibodies, Ripley, could be tested in an AA neutralization test without any further treatment [21].
  • A neutralization test with antibodies against G-, M- and GM-CSF indicated the stimulation for the proliferation of CMK and mouse bone marrow cells to be mediated partially by the CSFs [22].

Associations of Neutralization Tests with chemical compounds

  • Thrombin generation and neutralization test (TGNT) - a simple, practical, and sensitive assay for plasma heparin quantitation [23].
  • The results of the neutralization test also indicated that type C1 and D toxin preparations contain several common antigenic sites in their molecules [24].
  • Haemagglutination-inhibition and neutralization tests using rabbit sera to the E2 glycoprotein of TON, BB and PARA viruses showed them to be indistinguishable from each other and closely related to prototype subtype III virus Mucambo (MUC) [25].
  • An antigen-blocking or neutralization test (Abbott) confirmed the results of the Ortho assay [26].
  • The test used viral antigen purified in a sucrose density gradient, and the antibody titers detected were up to 100 times higher than those seen in the serum neutralization test [27].

Gene context of Neutralization Tests


Analytical, diagnostic and therapeutic context of Neutralization Tests


  1. Oncogenicity in marmosets of HL-23V, a type C oncornavirus isolated from human leukemic cells, and comparison with simian sarcoma virus type 1 (SSV-1/SSAV-1). Bergholz, C.M., Wolfe, L.G., Deinhardt, F., Thakkar, B., Marczynska, B. J. Natl. Cancer Inst. (1977) [Pubmed]
  2. Similarity of the outer capsid protein VP4 of the Gottfried strain of porcine rotavirus to that of asymptomatic human rotavirus strains. Gorziglia, M., Nishikawa, K., Hoshino, Y., Taniguchi, K. J. Virol. (1990) [Pubmed]
  3. Biological properties and viral surface antigens of Burkitt lymphoma- and mononucleosis- derived strains of Epstein-Barr virus released from transformed marmoset cells. Miller, G., Coope, D., Niederman, J., Pagano, J. J. Virol. (1976) [Pubmed]
  4. Cross-neutralization of hantaviruses with immune sera from experimentally infected animals and from hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome patients. Chu, Y.K., Jennings, G., Schmaljohn, A., Elgh, F., Hjelle, B., Lee, H.W., Jenison, S., Ksiazek, T., Peters, C.J., Rollin, P. J. Infect. Dis. (1995) [Pubmed]
  5. Development of a novel vaccinia-neutralization assay based on reporter-gene expression. Manischewitz, J., King, L.R., Bleckwenn, N.A., Shiloach, J., Taffs, R., Merchlinsky, M., Eller, N., Mikolajczyk, M.G., Clanton, D.J., Monath, T., Weltzin, R.A., Scott, D.E., Golding, H. J. Infect. Dis. (2003) [Pubmed]
  6. Monoclonal antibody with hemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol wt, surface-exposed immunogen of Treponema pallidum (Nichols). Jones, S.A., Marchitto, K.S., Miller, J.N., Norgard, M.V. J. Exp. Med. (1984) [Pubmed]
  7. Production of colony-stimulating factor by tumor cells and the factor-mediated induction of suppressor cells. Tsuchiya, Y., Igarashi, M., Suzuki, R., Kumagai, K. J. Immunol. (1988) [Pubmed]
  8. Radiculomyelitis following acute haemorrhagic conjunctivitis. Hung, T.P., Sung, S.M., Liang, H.C., Landsborough, D., Green, I.J. Brain (1976) [Pubmed]
  9. Radioimmunoassay of human serum antibody specific for adenovirus type 5-purified fiber. Scott, J.V., Dreesman, G.R., Spira, G., Kasel, J.A. J. Immunol. (1975) [Pubmed]
  10. Identification of chimpanzee Fab fragments by repertoire cloning and production of a full-length humanized immunoglobulin G1 antibody that is highly efficient for neutralization of dengue type 4 virus. Men, R., Yamashiro, T., Goncalvez, A.P., Wernly, C., Schofield, D.J., Emerson, S.U., Purcell, R.H., Lai, C.J. J. Virol. (2004) [Pubmed]
  11. Neutralization of yellow fever virus studied using monoclonal and polyclonal antibodies. Buckley, A., Gould, E.A. J. Gen. Virol. (1985) [Pubmed]
  12. Comparison of the latex agglutination test with the hemagglutination inhibition test, enzyme-linked immunosorbent assay, and neutralization test for detection of antibodies to rubella virus. Meegan, J.M., Evans, B.K., Horstmann, D.M. J. Clin. Microbiol. (1982) [Pubmed]
  13. The nature of Pseudomonas aeruginosa strain PAO bacteriophage receptors. Kropinski, A.M., Chan, L., Jarrell, K., Milazzo, F.H. Can. J. Microbiol. (1977) [Pubmed]
  14. Serological investigation of BK papovavirus infection in pregnant women and their offspring. Shah, K., Daniel, R., Madden, D., Stagno, S. Infect. Immun. (1980) [Pubmed]
  15. Antibody responses to different strains of coxsackie B4 virus in patients with newly diagnosed type I diabetes mellitus or aseptic meningitis. Frisk, G., Diderholm, H. J. Infect. (1997) [Pubmed]
  16. Anticoagulant activity of an anti-beta 2-glycoprotein I antibody is dependent on the presence of beta 2-glycoprotein I. Matsuda, J., Gohchi, K., Tsukamoto, M., Saitoh, N., Asami, K., Hashimoto, M. Am. J. Hematol. (1993) [Pubmed]
  17. A classical live attenuated vaccine for sheep pox. Bhanuprakash, V., Indrani, B.K., Hegde, R., Kumar, M.M., Moorthy, A.R. Tropical animal health and production. (2004) [Pubmed]
  18. Increased circulating interleukin-6 (IL-6) activity in endotoxin-challenged mice pretreated with anti-IL-6 antibody is due to IL-6 accumulated in antigen-antibody complexes. Martens, E., Dillen, C., Put, W., Heremans, H., van Damme, J., Billiau, A. Eur. J. Immunol. (1993) [Pubmed]
  19. Inhibition of liver metastases in murine colon adenocarcinoma by liposomes containing human C-reactive protein or crude lymphokine. Thombre, P.S., Deodhar, S.D. Cancer Immunol. Immunother. (1984) [Pubmed]
  20. A serologic study of California serogroup bunyaviruses in Sri Lanka. Arunagiri, C.K., Perera, L.P., Abeykoon, S.B., Peiris, J.S. Am. J. Trop. Med. Hyg. (1991) [Pubmed]
  21. Collagen-anti-collagen complexes in rheumatoid arthritis sera. Wozniczko-Orlowska, G., Milgrom, F. Int. Arch. Allergy Appl. Immunol. (1982) [Pubmed]
  22. Establishment of large cell lung cancer cell lines secreting hematopoietic factors inducing leukocytosis and thrombocytosis. Kimura, H., Yamaguchi, Y., Sun, L., Iwagami, S., Sugita, K. Jpn. J. Clin. Oncol. (1992) [Pubmed]
  23. Thrombin generation and neutralization test (TGNT) - a simple, practical, and sensitive assay for plasma heparin quantitation. Stavridis, I.C., Vorias, N.I. Thromb. Haemost. (1978) [Pubmed]
  24. Analysis of antigenicity of Clostridium botulinum type C1 and D toxins by polyclonal and monoclonal antibodies. Oguma, K., Murayama, S., Syuto, B., Iida, H., Kubo, S. Infect. Immun. (1984) [Pubmed]
  25. Comparative immunological and biochemical analyses of viruses in the Venezuelan equine encephalitis complex. Kinney, R.M., Trent, D.W., France, J.K. J. Gen. Virol. (1983) [Pubmed]
  26. Prevalence of non-A,non-B hepatitis/hepatitis C virus antibody in human immunoglobulins. Dodd, L.G., McBride, J.H., Gitnick, G.L., Howanitz, P.J., Rodgerson, D.O. Am. J. Clin. Pathol. (1992) [Pubmed]
  27. Enzyme-linked immunosorbent assay for serology of infectious bovine rhinotracheitis virus infections. Payment, P., Assaf, R., Trudel, M., Marois, P. J. Clin. Microbiol. (1979) [Pubmed]
  28. Establishment of a hepatocytic epithelial cell line from the murine fetal liver capable of promoting hemopoietic cell proliferation. Hata, M., Nanno, M., Doi, H., Satomi, S., Sakata, T., Suzuki, R., Itoh, T. J. Cell. Physiol. (1993) [Pubmed]
  29. A human amniotic cell line yielding high titres of human fibroblast interferon. Sorrentino, V., Di Francesco, P., Soria, M., Rossi, G.B. J. Gen. Virol. (1982) [Pubmed]
  30. Localization of mRNA for inflammatory cytokines in radicular cyst tissue by in situ hybridization, and induction of inflammatory cytokines by human gingival fibroblasts in response to radicular cyst contents. Honma, M., Hayakawa, Y., Kosugi, H., Koizumi, F. J. Oral Pathol. Med. (1998) [Pubmed]
  31. Antiproliferative and differentiating effects of polysaccharide fraction from fu-ling (Poria cocos) on human leukemic U937 and HL-60 cells. Chen, Y.Y., Chang, H.M. Food Chem. Toxicol. (2004) [Pubmed]
  32. Interleukin-8 production from cultured human dermal fibroblasts by stimulation with supernatant of cultured human epidermal cells. Morita, E., Yamada, S., Kimura, I., Nakamura, K., Sugita, Y., Yamamoto, S. Skin Pharmacol. (1993) [Pubmed]
  33. An analysis of the antibody response against West Nile virus E protein purified by SDS-PAGE indicates that this protein does not contain sequential epitopes for efficient induction of neutralizing antibodies. Wengler, G., Wengler, G. J. Gen. Virol. (1989) [Pubmed]
  34. Protective efficacy of vaccination in children in four episodes of natural varicella and zoster in the ward. Asano, Y., Nakayama, H., Yazaki, T., Ito, S., Isomura, S. Pediatrics (1977) [Pubmed]
  35. The Seattle virus watch. V. Epidemiologic observations of rhinovirus infections, 1965-1969, in families with young children. Fox, J.P., Cooney, M.K., Hall, C.E. Am. J. Epidemiol. (1975) [Pubmed]
  36. Production of interferon alpha by dengue virus-infected human monocytes. Kurane, I., Ennis, F.A. J. Gen. Virol. (1988) [Pubmed]
  37. Incidence of sand fly fever among Swedish United Nations soldiers on Cyprus during 1985. Eitrem, R., Vene, S., Niklasson, B. Am. J. Trop. Med. Hyg. (1990) [Pubmed]
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