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Gene Review

ZNF382  -  zinc finger protein 382

Homo sapiens

Synonyms: FLJ14686, KRAB/zinc finger suppressor protein 1, KS1, Multiple zinc finger and krueppel-associated box protein KS1, Zinc finger protein 382
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Disease relevance of ZNF382


High impact information on ZNF382

  • KS1 contains 10 C2H2 zinc fingers, a KRAB-A/B motif, and an ID sequence that has been shown previously to participate in growth factor-regulated gene expression [6].
  • Additional biochemical assays demonstrate that the KS1 KRAB domain interacts with the KAP-1 corepressor, and mutations that abolish this interaction alleviate KS1-mediated transcriptional repression [7].
  • Reporter assays demonstrate that KS1 represses the expression of promoters containing this DNA sequence [7].
  • Similarly, competitive binding studies between MAbs 17-1A and KS1/4 and MAb D612 revealed no similarity between the epitopes recognized by MAb D612 and MAbs 17-1A and KS1/4 [8].
  • The human immune response to KS1/4-desacetylvinblastine (LY256787) and KS1/4-desacetylvinblastine hydrazide (LY203728) in single and multiple dose clinical studies [9].

Biological context of ZNF382


Anatomical context of ZNF382

  • METHODS AND MATERIALS: CNE-1, CNE-2Z, and a normal human nasopharyngeal fibroblast strain, KS1, were infected with 2- and 6-plaque-forming units (pfu)/cell of Ad5CMV-p53, respectively [11].
  • Cytogenetic analysis was performed on a cell line, designated KS1, derived from a Krukenberg tumor [12].
  • Two transcript variants involving exon 7 were detected in K562 cells by RT-PCR, distinguishable from the wild-type transcript by deletions of 625 bp (KS32) and 988 bp (KS1) [13].

Associations of ZNF382 with chemical compounds

  • It is proposed that in these mutants the first step in erythromycin biosynthesis is the charging of KS1 with propionate directly from propionyl-CoA [14].
  • It has been most extensively demonstrated using a KS1 null mutation (KS1(0)) to block the first round of condensation in the biosynthesis of the erythromycin polyketide synthase (DEBS) for the production of analogues of its aglycone, 6-deoxyerythronolide B (6-dEB) [15].

Analytical, diagnostic and therapeutic context of ZNF382

  • Deletion and site-directed mutagenesis reveal that KS1 requires nine C-terminal zinc fingers and the KRAB domain for transcriptional repression through the KBE site, whereas the isolated zinc finger and linker region are dispensable for this function [7].
  • Western blotting analyses of tumor cell extracts utilizing KS1/4 reveal staining of a major Mr 40,000 band and a minor Mr 42,000 band [1].
  • Digestion of the KS1/4 immunoprecipitates with neuraminidase prior to two-dimensional analysis or immunoprecipitation of short pulse-labeled extracts reduces the number of spots to three each at the Mr 40,000 and Mr 42,000 positions [1].
  • Sequence analysis of the 5' and 3' noncoding regions of the KS1/4 cDNA revealed homologies to known proto-oncogenes and inflammatory mediators [3].
  • SAMPLES: Data came from a large-scale longitudinal study of children over KS1 (4-7 years) [16].


  1. Biosynthesis and glycosylation of the carcinoma-associated antigen recognized by monoclonal antibody KS1/4. Fernsten, P.D., Pekny, K.W., Reisfeld, R.A., Walker, L.E. Cancer Res. (1990) [Pubmed]
  2. Phase I clinical comparative study of monoclonal antibody KS1/4 and KS1/4-methotrexate immunconjugate in patients with non-small cell lung carcinoma. Elias, D.J., Hirschowitz, L., Kline, L.E., Kroener, J.F., Dillman, R.O., Walker, L.E., Robb, J.A., Timms, R.M. Cancer Res. (1990) [Pubmed]
  3. Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker. Perez, M.S., Walker, L.E. J. Immunol. (1989) [Pubmed]
  4. Real-time reverse transcription-PCR detects KS1/4 mRNA in mediastinal lymph nodes from patients with non-small cell lung cancer. Mitas, M., Cole, D.J., Hoover, L., Fraig, M.M., Mikhitarian, K., Block, M.I., Hoffman, B.J., Hawes, R.H., Gillanders, W.E., Wallace, M.B. Clin. Chem. (2003) [Pubmed]
  5. Bispecific-monoclonal-antibody-directed lysis of ovarian carcinoma cells by activated human T lymphocytes. Möller, S.A., Reisfeld, R.A. Cancer Immunol. Immunother. (1991) [Pubmed]
  6. KRAB-independent suppression of neoplastic cell growth by the novel zinc finger transcription factor KS1. Gebelein, B., Fernandez-Zapico, M., Imoto, M., Urrutia, R. J. Clin. Invest. (1998) [Pubmed]
  7. Sequence-specific transcriptional repression by KS1, a multiple-zinc-finger-Krüppel-associated box protein. Gebelein, B., Urrutia, R. Mol. Cell. Biol. (2001) [Pubmed]
  8. Characterization of the colorectal carcinoma-associated antigen defined by monoclonal antibody D612. Fernsten, P.D., Primus, F.J., Greiner, J.W., Simpson, J.F., Schlom, J. Cancer Res. (1991) [Pubmed]
  9. The human immune response to KS1/4-desacetylvinblastine (LY256787) and KS1/4-desacetylvinblastine hydrazide (LY203728) in single and multiple dose clinical studies. Petersen, B.H., DeHerdt, S.V., Schneck, D.W., Bumol, T.F. Cancer Res. (1991) [Pubmed]
  10. Expression of a novel Krüpple-like zinc-finger gene, ZNF382, in human heart. Luo, K., Yuan, W., Zhu, C., Li, Y., Wang, Y., Zeng, W., Jiao, W., Liu, M., Wu, X. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
  11. The effects of combining ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma. Li, J.H., Lax, S.A., Kim, J., Klamut, H., Liu, F.F. Int. J. Radiat. Oncol. Biol. Phys. (1999) [Pubmed]
  12. Cytogenetic analysis of a cell line established from a Krukenberg tumor. Sheer, D., Gorman, P.A., Whelan, R., Hill, B.T. Cancer Genet. Cytogenet. (1990) [Pubmed]
  13. Structure and organization of the human reduced folate carrier gene. Zhang, L., Wong, S.C., Matherly, L.H. Biochim. Biophys. Acta (1998) [Pubmed]
  14. The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraea. Pereda, A., Summers, R.G., Stassi, D.L., Ruan, X., Katz, L. Microbiology (Reading, Engl.) (1998) [Pubmed]
  15. Precursor-directed biosynthesis of 6-deoxyerythronolide B analogues is improved by removal of the initial catalytic sites of the polyketide synthase. Ward, S.L., Desai, R.P., Hu, Z., Gramajo, H., Katz, L. J. Ind. Microbiol. Biotechnol. (2007) [Pubmed]
  16. Class size, pupil attentiveness and peer relations. Blatchford, P., Edmonds, S., Martin, C. The British journal of educational psychology. (2003) [Pubmed]
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