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RPL10  -  ribosomal 60S subunit protein L10

Saccharomyces cerevisiae S288c

Synonyms: 60S ribosomal protein L10, GRC5, L9, QSR1, Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein, ...
 
 
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High impact information on RPL10

  • Mutations in LSG1 or RPL10 blocked Nmd3-GFP shuttling into the nucleus and export of pre-60S subunits from the nucleus [1].
  • The defect in Nmd3p recycling and the block in 60S export in both lsg1 and rpl10 mutants was also suppressed by mutant Nmd3 proteins that showed reduced binding to 60S subunits in vitro [1].
  • Interestingly, free 60S ribosomal subunits of a rsa1 null mutant strain that was grown for two generations at 37 degrees C are practically devoid of the 60S-ribosomal-subunit protein Qsr1p/Rpl10p, which is required for joining of 60S and 40S subunits (D. P. Eisinger, F. A. Dick, and B. L. Trumpower, Mol. Cell. Biol. 17:5136-5145, 1997) [2].
  • Loss of SQT1 function by down regulation from an inducible promoter results in formation of half-mer polyribosomes and decreased Qsr1p levels on free 60S subunits [3].
  • The suppressor of qsr1 truncation mutants, SQT1, is an essential gene, which encodes a 47.1-kDa protein containing multiple WD repeats and which interacts strongly with Qsr1p in a yeast two-hybrid system [3].
 

Biological context of RPL10

  • Three of the four mutants (mrt4, grc5, and sla2) were not defective in protein synthesis, suggesting that these strains contain mutations in factors that may play a specific role in mRNA decay [4].
  • Over-expression of RPL10 suppresses the half-mer phenotype of the Deltarpl29 strain, but does not correct the growth defect of the deletion strain [5].
  • SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1 [3].
  • When one chromosomal copy of QSR1 was deleted in a diploid yeast strain, haploid spores derived therefrom and carrying the deletion were unable to grow on fermentable or non-fermentable carbon sources [6].
  • Our results support the view that Grc5p/RpL10p links ribosome function to membrane turnover and cell surface biogenesis [7].
 

Anatomical context of RPL10

 

Associations of RPL10 with chemical compounds

  • Two-dimensional polyacrylamide-gel electrophoretic analysis of yeast ribosomal proteins labelled in vivo with 32PO43- revealed that the proteins S2 and S10 of the 40S ribosomal subunit, and the proteins L9, L30, L44 and L45 of the 60S ribosomal subunit, are phosphorylated in vivo [9].
 

Physical interactions of RPL10

  • Translational regulator RpL10p/Grc5p interacts physically and functionally with Sed1p, a dynamic component of the yeast cell surface [7].
 

Other interactions of RPL10

  • The function of the QSR1-encoded protein (Qsr1p) and its relationship to the QCR6-encoded protein are unknown [8].
  • We identified recessive lethal alleles of the Saccharomyces cerevisiae QM homolog GRC5 that increased GCN4 expression when present in multiple copies [10].
  • GRC5 and NMD3 function in translational control of gene expression and interact genetically [11].

References

  1. Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p. Hedges, J., West, M., Johnson, A.W. EMBO J. (2005) [Pubmed]
  2. Synthetic lethality with conditional dbp6 alleles identifies rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits. Kressler, D., Doère, M., Rojo, M., Linder, P. Mol. Cell. Biol. (1999) [Pubmed]
  3. SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1. Eisinger, D.P., Dick, F.A., Denke, E., Trumpower, B.L. Mol. Cell. Biol. (1997) [Pubmed]
  4. Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover. Zuk, D., Belk, J.P., Jacobson, A. Genetics (1999) [Pubmed]
  5. RPL29 codes for a non-essential protein of the 60S ribosomal subunit in Saccharomyces cerevisiae and exhibits synthetic lethality with mutations in genes for proteins required for subunit coupling. DeLabre, M.L., Kessl, J., Karamanou, S., Trumpower, B.L. Biochim. Biophys. Acta (2002) [Pubmed]
  6. QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc1 complex, is homologous to a gene implicated in eukaryotic cell differentiation. Tron, T., Yang, M., Dick, F.A., Schmitt, M.E., Trumpower, B.L. J. Biol. Chem. (1995) [Pubmed]
  7. Translational regulator RpL10p/Grc5p interacts physically and functionally with Sed1p, a dynamic component of the yeast cell surface. Oender, K., Loeffler, M., Doppler, E., Eder, M., Lach, S., Heinrich, F., Karl, T., Moesl, R., Hundsberger, H., Klade, T., Eckl, P., Dickinson, J.R., Breitenbach, M., Koller, L. Yeast (2003) [Pubmed]
  8. QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc1 complex, codes for a 60 S ribosomal subunit protein. Dick, F.A., Karamanou, S., Trumpower, B.L. J. Biol. Chem. (1997) [Pubmed]
  9. Modification of yeast ribosomal proteins. Phosphorylation. Kruiswijk, T., de Hey, J.T., Planta, R.J. Biochem. J. (1978) [Pubmed]
  10. Ribosomal protein L9 is the product of GRC5, a homolog of the putative tumor suppressor QM in S. cerevisiae. Nika, J., Erickson, F.L., Hannig, E.M. Yeast (1997) [Pubmed]
  11. GRC5 and NMD3 function in translational control of gene expression and interact genetically. Karl, T., Onder, K., Kodzius, R., Pichová, A., Wimmer, H., Th r, A., Hundsberger, H., Löffler, M., Klade, T., Beyer, A., Breitenbach, M., Koller, L. Curr. Genet. (1999) [Pubmed]
 
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