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Gene Review

CKI1  -  bifunctional choline kinase/ethanolamine...

Saccharomyces cerevisiae S288c

Synonyms: CKI, Choline kinase, L3130, L9606.8, YLR133W
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Disease relevance of CKI1


High impact information on CKI1

  • The antagonistic action of the CKI gene product on SEC14p function revealed a previously unsuspected influence of biosynthetic activities of the CDP-choline pathway for PC biosynthesis on yeast Golgi function and indicated that SEC14p controls the phospholipid content of yeast Golgi membranes in vivo [5].
  • Our data support a model in which CDC-14 promotes a hypophosphorylated and stable form of CKI-1 required for developmentally programmed cell-cycle arrest [6].
  • HRR25 encodes a protein kinase that is closely related to bovine casein kinase I (CKI) [7].
  • Casein kinase I (CKI) is a class of protein kinases ubiquitous to all eukaryotic cells [8].
  • Surprisingly, we find that a region of Pho81 containing 80 amino acids C terminal to the ankyrin repeats is necessary and sufficient for Pho81's CKI function [9].

Chemical compound and disease context of CKI1

  • The E. coli produced forms of Hhp1, Hhp2, and Cki1 were autophosphorylated on tyrosine, and both Hhp1 and Hhp2 were capable of phosphorylating the tyrosine-protein kinase synthetic peptide substrate polymer poly-E4Y1 [10].

Biological context of CKI1


Anatomical context of CKI1

  • These extensions do not show any sequence similarity to nonplant CKI isoforms, such as rat testis CKI delta, which is their closest isolated homolog, or to yeast CKI isoforms [13].
  • The acidic ribosomal proteins and the protein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI [14].

Associations of CKI1 with chemical compounds


Other interactions of CKI1


Analytical, diagnostic and therapeutic context of CKI1


  1. Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli. Hosaka, K., Kodaki, T., Yamashita, S. J. Biol. Chem. (1989) [Pubmed]
  2. Molecular cloning, characterization, and expression in Escherichia coli of a cDNA encoding mammalian choline kinase. Uchida, T., Yamashita, S. J. Biol. Chem. (1992) [Pubmed]
  3. Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation. Hosaka, K., Tanaka, S., Nikawa, J., Yamashita, S. FEBS Lett. (1992) [Pubmed]
  4. Incorporation of choline into Streptococcus pneumoniae cell wall antigens: evidence for choline kinase activity. Whiting, G.C., Gillespie, S.H. FEMS Microbiol. Lett. (1996) [Pubmed]
  5. Mutations in the CDP-choline pathway for phospholipid biosynthesis bypass the requirement for an essential phospholipid transfer protein. Cleves, A.E., McGee, T.P., Whitters, E.A., Champion, K.M., Aitken, J.R., Dowhan, W., Goebl, M., Bankaitis, V.A. Cell (1991) [Pubmed]
  6. The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans. Saito, R.M., Perreault, A., Peach, B., Satterlee, J.S., van den Heuvel, S. Nat. Cell Biol. (2004) [Pubmed]
  7. The budding yeast HRR25 gene product is a casein kinase I isoform. DeMaggio, A.J., Lindberg, R.A., Hunter, T., Hoekstra, M.F. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  8. Cell cycle-dependent localization of casein kinase I to mitotic spindles. Brockman, J.L., Gross, S.D., Sussman, M.R., Anderson, R.A. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  9. Functional analysis of the cyclin-dependent kinase inhibitor Pho81 identifies a novel inhibitory domain. Huang, S., Jeffery, D.A., Anthony, M.D., O'Shea, E.K. Mol. Cell. Biol. (2001) [Pubmed]
  10. Budding and fission yeast casein kinase I isoforms have dual-specificity protein kinase activity. Hoekstra, M.F., Dhillon, N., Carmel, G., DeMaggio, A.J., Lindberg, R.A., Hunter, T., Kuret, J. Mol. Biol. Cell (1994) [Pubmed]
  11. Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Wang, P.C., Vancura, A., Mitcheson, T.G., Kuret, J. Mol. Biol. Cell (1992) [Pubmed]
  12. Role of the yeast phosphatidylinositol/phosphatidylcholine transfer protein (Sec14p) in phosphatidylcholine turnover and INO1 regulation. Patton-Vogt, J.L., Griac, P., Sreenivas, A., Bruno, V., Dowd, S., Swede, M.J., Henry, S.A. J. Biol. Chem. (1997) [Pubmed]
  13. Multiple isoforms of Arabidopsis casein kinase I combine conserved catalytic domains with variable carboxyl-terminal extensions. Klimczak, L.J., Farini, D., Lin, C., Ponti, D., Cashmore, A.R., Giuliano, G. Plant Physiol. (1995) [Pubmed]
  14. Protein kinases CKI and CKII are implicated in modification of ribosomal proteins of the yeast Trichosporon cutaneum. Wojda, I., Cytryńska, M., Frajnt, M., Jakubowicz, T. Acta Biochim. Pol. (2002) [Pubmed]
  15. Isolation and characterization of the Saccharomyces cerevisiae EKI1 gene encoding ethanolamine kinase. Kim, K., Kim, K.H., Storey, M.K., Voelker, D.R., Carman, G.M. J. Biol. Chem. (1999) [Pubmed]
  16. Phosphorylation of the yeast choline kinase by protein kinase C. Identification of Ser25 and Ser30 as major sites of phosphorylation. Choi, M.G., Kurnov, V., Kersting, M.C., Sreenivas, A., Carman, G.M. J. Biol. Chem. (2005) [Pubmed]
  17. Functional redundancy of CDP-ethanolamine and CDP-choline pathway enzymes in phospholipid biosynthesis: ethanolamine-dependent effects on steady-state membrane phospholipid composition in Saccharomyces cerevisiae. McGee, T.P., Skinner, H.B., Bankaitis, V.A. J. Bacteriol. (1994) [Pubmed]
  18. Regulation of the Saccharomyces cerevisiae CKI1-encoded choline kinase by zinc depletion. Soto, A., Carman, G.M. J. Biol. Chem. (2008) [Pubmed]
  19. Construction of a plasmid carrying both CTP synthetase and a fused gene formed from cholinephosphate cytidylyltransferase and choline kinase genes and its application to industrial CDP-choline production: enzymatic production of CDP-choline from orotic acid (Part II). Fujio, T., Teshiba, S., Maruyama, A. Biosci. Biotechnol. Biochem. (1997) [Pubmed]
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