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Gene Review

FDH1  -  formate dehydrogenase (NAD+)

Saccharomyces cerevisiae S288c

Synonyms: Formate dehydrogenase 1, NAD-dependent formate dehydrogenase 1, YOR388C
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Disease relevance of FDH1

  • Enzymatically active FDH was produced to 15% of soluble E. coli protein [1].
  • To confirm the hypothesis of a thiol-coupled inactivation process, both cysteine residues in the primary structure of the enzyme have been exchanged by site-directed mutagenesis using a homology model based on the 3D structure of FDH from Pseudomonas sp. 101 and from related dehydrogenases [2].
  • An expression system has been developed for the methylotrophic yeast Hansenula polymorpha and used to co-express both the L (preS1-S2-S) and S hepatitis B surface antigens (HBsAg) under the control of strong methanol-inducible promoters derived from the methanol oxidase and from the formate dehydrogenase genes [3].

High impact information on FDH1


Biological context of FDH1


Anatomical context of FDH1


Associations of FDH1 with chemical compounds

  • Although the growth of the fdh1 delta mutant was impaired and the level of formate was higher in the fdh1 delta mutant than in the wild-type strain, the growth defect caused by FDH1 gene disruption was small and less severe than that caused by growth on methanol [12].
  • Induction of FDH1 was not repressed in the presence of glucose when cells were grown on methylamine, choline, or formate, and expression of FDH1 was shown to be regulated at the mRNA level [12].
  • Expression of FDH1 was found to be induced by choline or methylamine (used as a nitrogen source), as well as by methanol (used as a carbon source) [12].
  • Cis-acting elements sufficient for induction of FDH1 expression by formate in the methylotrophic yeast Candida boidinii [8].
  • Growth on methylamine or choline as a nitrogen source in a batch culture was compared between the wild type and the fdh1 delta mutant [12].

Other interactions of FDH1

  • Irrespective of whether induction was achieved with methanol or formate, the UAS-FM element enhanced the level of induction of the FDH1 promoter in a manner dependent on the number of copies, but independent of their orientation, and also converted the ACT1 promoter from a constitutive into an inducible element [8].

Analytical, diagnostic and therapeutic context of FDH1


  1. Isolation, sequence and overexpression of the gene encoding NAD-dependent formate dehydrogenase from the methylotrophic yeast Candida methylica. Allen, S.J., Holbrook, J.J. Gene (1995) [Pubmed]
  2. Stabilization of NAD-dependent formate dehydrogenase from Candida boidinii by site-directed mutagenesis of cysteine residues. Slusarczyk, H., Felber, S., Kula, M.R., Pohl, M. Eur. J. Biochem. (2000) [Pubmed]
  3. Simultaneous expression of the S and L surface antigens of hepatitis B, and formation of mixed particles in the methylotrophic yeast, Hansenula polymorpha. Janowicz, Z.A., Melber, K., Merckelbach, A., Jacobs, E., Harford, N., Comberbach, M., Hollenberg, C.P. Yeast (1991) [Pubmed]
  4. Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris. Tuttle, D.L., Dunn, W.A. J. Cell. Sci. (1995) [Pubmed]
  5. Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae. Serov, A.E., Popova, A.S., Fedorchuk, V.V., Tishkov, V.I. Biochem. J. (2002) [Pubmed]
  6. Effect of pressure on deuterium isotope effects of formate dehydrogenase. Quirk, D.J., Northrop, D.B. Biochemistry (2001) [Pubmed]
  7. Functional analysis of structural genes for NAD(+)-dependent formate dehydrogenase in Saccharomyces cerevisiae. Overkamp, K.M., Kötter, P., van der Hoek, R., Schoondermark-Stolk, S., Luttik, M.A., van Dijken, J.P., Pronk, J.T. Yeast (2002) [Pubmed]
  8. Cis-acting elements sufficient for induction of FDH1 expression by formate in the methylotrophic yeast Candida boidinii. Komeda, T., Yurimoto, H., Kato, N., Sakai, Y., Kondo, K. Mol. Genet. Genomics (2003) [Pubmed]
  9. Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae. Sasnauskas, K., Jomantiene, R., Januska, A., Lebediene, E., Lebedys, J., Janulaitis, A. Gene (1992) [Pubmed]
  10. Steady-state kinetics of formaldehyde dehydrogenase and formate dehydrogenase from a methanol-utilizing yeast, Candida boidinii. Kato, N., Sahm, H., Wagner, F. Biochim. Biophys. Acta (1979) [Pubmed]
  11. Degradation of microbodies in relation to activities of alcohol oxidase and catalase in Candida boidinii. Bormann, C., Sahm, H. Arch. Microbiol. (1978) [Pubmed]
  12. Regulation of the formate dehydrogenase gene, FDH1, in the methylotrophic yeast Candida boidinii and growth characteristics of an FDH1-disrupted strain on methanol, methylamine, and choline. Sakai, Y., Murdanoto, A.P., Konishi, T., Iwamatsu, A., Kato, N. J. Bacteriol. (1997) [Pubmed]
  13. Protein engineering of formate dehydrogenase. Tishkov, V.I., Popov, V.O. Biomol. Eng. (2006) [Pubmed]
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