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Gene Review

RAD10  -  Rad10p

Saccharomyces cerevisiae S288c

Synonyms: DNA repair protein RAD10, YML095C
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Disease relevance of RAD10

  • The collected experiments reveal a profound toxicity of strand breaks with irreparable 3' blocking lesions, and extend the function of the Rad1/Rad10 salvage pathway to 3'-phosphates [1].
  • RAD10 protein expressed in Escherichia coli maxicells has a mol. wt of approximately 30 kd measured by gel electrophoresis [2].

High impact information on RAD10


Biological context of RAD10


Anatomical context of RAD10

  • Following transfection with the RAD10 gene, three independently isolated excision repair-defective CHO cell lines from the same genetic complementation group (complementation group 2) showed partial complementation of sensitivity to killing by UV radiation and to the DNA cross-linking agent mitomycin C [6].
  • Using the 'two-hybrid' genetic assay system we now report that Rad1 and Rad10 proteins are subunits of a specific complex in the cell nucleus [11].

Associations of RAD10 with chemical compounds

  • The inactivation of Rad1 or Rad10 in GCR mutator strains also slightly enhanced methyl methanesulfonate sensitivity [10].
  • The middle portion of the RAD10 protein, which is highly basic and also contains eight of the total of 10 tyrosine residues present in the protein, may be involved in DNA binding by ionic interactions and tyrosine intercalation between the bases of DNA [12].
  • We characterize the RAD1/RAD10 endonuclease activity on both single-stranded and double-stranded DNAs, using agarose gel electrophoresis and trichloroacetic acid precipitation [13].
  • However, in contrast to RAD2 [Robinson et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 1842-1846], RAD10 is not induced following exposure of cells to the DNA-damaging agent 4-nitroquinoline 1-oxide [14].
  • Two rad mutants of yeast, rad10 and rad16, are shown to be defective in the removal of UV-induced pyrimidine dimers since DNAs obtained from irradiated cells following a post-irradiation incubation in the dark still retain UV-endonuclease-sensitive sites [15].

Physical interactions of RAD10

  • The RAD1/RAD10 complex is highly stable, being refractory to 1 M NaCl and to low concentrations of SDS [7].
  • Rad1-Rad10 forms a ternary complex with the DNA damage recognition protein Rad14, providing a means for targeting this nuclease to the damage site [16].

Other interactions of RAD10

  • Here, we show that the RAD1 and RAD10 proteins are complexed with each other in vivo [7].
  • These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway [9].
  • Thus, the recombination repair defects of rad1 and rad10 may confer an additional synergistic effect when combined with the apn1 mutation [17].
  • Double strand break-induced plasmid integration was also decreased by disruption of RAD10, which forms a complex with RAD1; disruption of RAD4 had no effect [18].
  • The differential sensitivity of wild-type cells and those lacking Ixr1 persisted in the rad1 and rad10 strains, however, indicating that these two proteins act at a stage in the excision repair pathway where damage recognition is less critical [19].

Analytical, diagnostic and therapeutic context of RAD10


  1. The role of yeast DNA 3'-phosphatase Tpp1 and rad1/Rad10 endonuclease in processing spontaneous and induced base lesions. Karumbati, A.S., Deshpande, R.A., Jilani, A., Vance, J.R., Ramotar, D., Wilson, T.E. J. Biol. Chem. (2003) [Pubmed]
  2. Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli. Weiss, W.A., Friedberg, E.C. EMBO J. (1985) [Pubmed]
  3. Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10. van Duin, M., de Wit, J., Odijk, H., Westerveld, A., Yasui, A., Koken, H.M., Hoeijmakers, J.H., Bootsma, D. Cell (1986) [Pubmed]
  4. Holliday junction cleavage by yeast Rad1 protein. Habraken, Y., Sung, P., Prakash, L., Prakash, S. Nature (1994) [Pubmed]
  5. Requirement of yeast Rad1-Rad10 nuclease for the removal of 3'-blocked termini from DNA strand breaks induced by reactive oxygen species. Guzder, S.N., Torres-Ramos, C., Johnson, R.E., Haracska, L., Prakash, L., Prakash, S. Genes Dev. (2004) [Pubmed]
  6. A yeast DNA repair gene partially complements defective excision repair in mammalian cells. Lambert, C., Couto, L.B., Weiss, W.A., Schultz, R.A., Thompson, L.H., Friedberg, E.C. EMBO J. (1988) [Pubmed]
  7. Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10. Bailly, V., Sommers, C.H., Sung, P., Prakash, L., Prakash, S. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  8. Role of the Rad1 and Rad10 proteins in nucleotide excision repair and recombination. Davies, A.A., Friedberg, E.C., Tomkinson, A.E., Wood, R.D., West, S.C. J. Biol. Chem. (1995) [Pubmed]
  9. RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination. Schiestl, R.H., Prakash, S. Mol. Cell. Biol. (1990) [Pubmed]
  10. The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiae. Hwang, J.Y., Smith, S., Myung, K. Genetics (2005) [Pubmed]
  11. Yeast DNA recombination and repair proteins Rad1 and Rad10 constitute a complex in vivo mediated by localized hydrophobic domains. Bardwell, A.J., Bardwell, L., Johnson, D.K., Friedberg, E.C. Mol. Microbiol. (1993) [Pubmed]
  12. Nucleotide sequence of the RAD10 gene of Saccharomyces cerevisiae. Reynolds, P., Prakash, L., Dumais, D., Perozzi, G., Prakash, S. EMBO J. (1985) [Pubmed]
  13. Purification and characterization of the Saccharomyces cerevisiae RAD1/RAD10 endonuclease. Sung, P., Reynolds, P., Prakash, L., Prakash, S. J. Biol. Chem. (1993) [Pubmed]
  14. Characterization of the RAD10 gene of Saccharomyces cerevisiae and purification of Rad10 protein. Bardwell, L., Burtscher, H., Weiss, W.A., Nicolet, C.M., Friedberg, E.C. Biochemistry (1990) [Pubmed]
  15. Defective thymine dimer excision in radiation-sensitive mutants rad10 and rad16 of Saccharomyces cerevisiae. Prakash, L. Mol. Gen. Genet. (1977) [Pubmed]
  16. Transcription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: implications for nucleotide excision repair and Cockayne syndrome. Habraken, Y., Sung, P., Prakash, S., Prakash, L. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  17. Synergism between yeast nucleotide and base excision repair pathways in the protection against DNA methylation damage. Xiao, W., Chow, B.L. Curr. Genet. (1998) [Pubmed]
  18. Single strand and double strand DNA damage-induced reciprocal recombination in yeast. Dependence on nucleotide excision repair and RAD1 recombination. Saffran, W.A., Greenberg, R.B., Thaler-Scheer, M.S., Jones, M.M. Nucleic Acids Res. (1994) [Pubmed]
  19. The HMG-domain protein Ixr1 blocks excision repair of cisplatin-DNA adducts in yeast. McA'Nulty, M.M., Lippard, S.J. Mutat. Res. (1996) [Pubmed]
  20. Pol32, a subunit of Saccharomyces cerevisiae DNA polymerase delta, suppresses genomic deletions and is involved in the mutagenic bypass pathway. Huang, M.E., Rio, A.G., Galibert, M.D., Galibert, F. Genetics (2002) [Pubmed]
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