Disease relevance of DLEU2

• BCMSUN, a candidate gene for B-cell chronic lymphocytic leukemia and mantle-cell lymphoma, has an independently expressed homolog on 1p22-p31, BCMSUN-like [1].
• Monoclonal antibody 1B4, previously shown to be protective in vivo and to cross-react with both virally encoded and normal host cell proteins, was used to screen a lambda gt11 cDNA derived from mRNA harvested from mouse embryo fibroblasts 24 hr after infection with murine cytomegalovirus (MCMV) [2].

High impact information on DLEU2

• The MDR contains 2 pseudogenes and 3 transcribed genes: CAR, encoding a putative RING-finger containing protein; 1B4/Leu2, generating noncoding transcripts; and EST70/Leu1, probably representing another noncoding gene (longest open reading frame of 78 codons) [3].
• For the two genes residing in the proximal subregion, initially named LEU1 and LEU2, a pathogenic role has not yet been established [4].
• Two novel 3' exons of LEU2 were also identified and are present in both LEU2 and ALT1 transcripts [5].
• Aggregated C1q-induced platelet aggregation was also inhibited by a mAb (1B4) directed against the recombinant gC1qR [6].
• The murine anti-CD18 mAb 1B4 has been humanized using CDR grafting [7].

Biological context of DLEU2

• The Leu1 and Leu2 genes show little homology to previously published genes at the nucleotide and expected translated amino acid sequence level [8].
• 3. Therefore, analysis of the candidate tumor-suppressor gene BCMSUN at 13q14.3 must be based on assays that distinguish between the 2 homologous genes [1].
• Control proteins including human serum albumin, hemoglobin, Fab fragments of anti-fibronectin, anti-beta 2 microglobulin, and MOPC 21, and Fc fragments of 1B4 and MOPC 21 produced no significant stimulation of phagocytosis, nor did F(ab')2 fragments of monoclonal anti-CR3, M1/70 [9].
• An epitope recognized by MAb 1B4 was identified using recombinant pGEX plasmids expressing fusion proteins representing the N-terminal region of the MCMV UL25 protein [2].
• This elevation was observed for both MoAb 1B4, which competes for the ICC binding site of ECR1, and for MoAb HB8592, which does not, but the time course for the increase in binding of the two MoAbs was different, in that the epitope recognized by MoAb 1B4 increased more rapidly [10].

Anatomical context of DLEU2

• Penetration of HEp-2 epithelial cells by C. jejuni was significantly inhibited (P less than .05) with C. jejuni lysate and a monoclonal antibody (MAb 1B4) in competitive inhibition assays [11].
• Immunogold electron microscopy revealed that MAb 1B4 bound to the flagella and cell surface of low-passage (invasive) C. jejuni M 96, whereas only the flagella of high-passage (noninvasive) C. jejuni M 96 were labeled [11].
• The anti-CR1 mAb 1B4 and 3D9, which block recognition of ligand by CR1, did not bind to chimpanzee erythrocytes and bound partially to rhesus and cynomolgus monkey erythrocytes [12].
• Two of the antibodies (1B4 and 6E6) had binding stoichiometries that were approximately 2-fold lower than those of other mAb (10B2, 12D9 and 7G7), suggesting either that gp190 is present as a pre-associated homodimer in the cell membrane or that part of gp190 is pre-associated with another component [13].
• Cultures were then inverted, fixed with methanol, and adherent neutrophils labeled with 1B4 mouse monoclonal anti-human neutrophil antibody followed by fluorescein-labeled sheep anti-mouse IgG [14].

Associations of DLEU2 with chemical compounds

• In contrast, Hst5 and 1B4, a salivary proline-rich protein, had little if any effect on the transepithelial transport of the flavonoid quercetin in apical to basolateral as well as basolateral to apical direction [15].

Other interactions of DLEU2

• Furthermore, our work allowed us to identify new alternative transcripts, spanning core regions, of the previously defined candidate genes DLEU1 and DLEU2 [16].
• Distinct organization of the candidate tumor suppressor gene RFP2 in human and mouse: multiple mRNA isoforms in both species- and human-specific antisense transcript RFP2OS [17].
• We identified a homolog of BCMSUN, termed BCMSUNL (for BCMSUN-like) [1].

Analytical, diagnostic and therapeutic context of DLEU2

• We report the histochemical application of three monoclonal antibodies (Mab) produced in this laboratory, 1B4, 2E1, and 4A11, which are monospecific to GFAP by radioimmunoassay, immunoblot electrophoresis, and immunoperoxidase histochemistry [18].
• Western blot analysis revealed that MAb 1B4 identified an epitope on antigens of 64-44 kDa in lysates prepared from invasive and noninvasive isolates [11].

References