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Gene Review

recA  -  recombinase A

Escherichia coli O157:H7 str. Sakai

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Disease relevance of recA


High impact information on recA

  • To obtain structural information about the self-association of the protein RecA, we studied urea denaturation of RecA by circular dichroism spectroscopy and gel filtration [6].
  • On an adduct basis N-Acetoxy-N-2-acetylaminofluorene was shown to induce the protein RecA at a similar level as U.V. On the other hand, N-hydroxy-N-2-aminofluorene shows only a poor induction capacity of the RecA protein [7].
  • We also discovered that recA was expressed at high constitutive levels, due to constitutive LexA cleavage by RecA, with little induction following DNA damage [8].
  • Analyses of the Brucella spp. genomes and our molecular studies documented the presence of only one recA gene, suggesting a RecA-independent repair process [8].
  • The appearance of Arg(+) mutants on the plates with streptomycin was not significantly altered by recA, rpoS or dps mutations [9].

Biological context of recA

  • Interestingly, deletion did not occur in A. tumefaciens strain AGL1 with a recA mutation in C58 chromosome, implying a clear role for recombination in deletion [10].
  • A 100-fold reduction in the recombination rate was observed in a recA mutant strain even though crossing-over was still efficient [11].
  • In Escherichia coli the LexA protein acts as a recA repressor and is able, in response to DNA damage, of RecA-promoted self-cleavage, thus allowing recA transcription [4].
  • The analysis of deletion mutants and of translational gene fusions showed that a DNA region of 83 base pairs, containg the recA promoter and the transcriptional start site, is sufficient to ensure normal expression of the gene [4].
  • Base up-regulated core genes for maltodextrin transport (lamB, mal), ATP synthase (atp), and DNA repair (recA, mutL) [12].

Anatomical context of recA

  • Using single-stranded DNA, non-coated constructs produced a transgene integration rate of 0.5%, while none of the 1040 zygotes injected with recombinase A-coated constructs produced transgenic pups [13].

Analytical, diagnostic and therapeutic context of recA


  1. Regulated site-specific recombination of the she pathogenicity island of Shigella flexneri. Sakellaris, H., Luck, S.N., Al-Hasani, K., Rajakumar, K., Turner, S.A., Adler, B. Mol. Microbiol. (2004) [Pubmed]
  2. New rapid and simple methods for detection of bacteria and determination of their antibiotic susceptibility by using phage mutants. Ulitzur, N., Ulitzur, S. Appl. Environ. Microbiol. (2006) [Pubmed]
  3. Isolation of bacteriocinogenic Lactobacillus plantarum strains from ben saalga, a traditional fermented gruel from Burkina Faso. Omar, N.B., Abriouel, H., Lucas, R., Mart??nez-Ca??amero, M., Guyot, J.P., G??lvez, A. Int. J. Food Microbiol. (2006) [Pubmed]
  4. Transcriptional analysis of the recA gene of Streptococcus thermophilus. Giliberti, G., Baccigalupi, L., Cordone, A., Ricca, E., De Felice, M. Microb. Cell Fact. (2006) [Pubmed]
  5. RecA-independent pathways of lambdoid prophage induction in Escherichia coli. Rozanov, D.V., D'Ari, R., Sineoky, S.P. J. Bacteriol. (1998) [Pubmed]
  6. Local folding of the N-terminal domain of Escherichia coli RecA controls protein-protein interaction. Masui, R., Mikawa, T., Kuramitsu, S. J. Biol. Chem. (1997) [Pubmed]
  7. Different levels of induction of RecA protein in E. coli (PQ 10) after treatment with two related carcinogens. Salles, B., Lang, M.C., Freund, A.M., Paoletti, C., Daune, M., Fuchs, R.P. Nucleic Acids Res. (1983) [Pubmed]
  8. RecA and RadA proteins of Brucella abortus do not perform overlapping protective DNA repair functions following oxidative burst. Roux, C.M., Booth, N.J., Bellaire, B.H., Gee, J.M., Roop, R.M., Kovach, M.E., Tsolis, R.M., Elzer, P.H., Ennis, D.G. J. Bacteriol. (2006) [Pubmed]
  9. Mistranslation and genetic variability The effect of streptomycin. Nagel, R., Chan, A. Mutat. Res. (2006) [Pubmed]
  10. Factors contributing to deletion within Mungbean yellow mosaic virus partial dimers in binary vectors used for agroinoculation. Shivaprasad, P.V., Thomas, M., Balamani, V., Biswas, D., Vanitharani, R., Karthikeyan, A.S., Veluthambi, K. J. Virol. Methods (2006) [Pubmed]
  11. Gene reconstitution using high efficiency homologous recombination between a bacteriophage fd and a plasmid. Br??do, A., Mathonet, P., Deschuyteneer, G., Soumillion, P. J. Biotechnol. (2006) [Pubmed]
  12. Oxygen limitation modulates pH regulation of catabolism and hydrogenases, multidrug transporters, and envelope composition in Escherichia coli K-12. Hayes, E.T., Wilks, J.C., Sanfilippo, P., Yohannes, E., Tate, D.P., Jones, B.D., Radmacher, M.D., BonDurant, S.S., Slonczewski, J.L. BMC Microbiol. (2006) [Pubmed]
  13. The effect of coating single- and double-stranded DNA with the recombinase A protein of Escherichia coli on transgene integration in mice. Mason, J.B., Najarian, J.G., Anderson, G.B., Murray, J.D., Maga, E.A. Transgenic Res. (2006) [Pubmed]
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