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Gene Review

cysH  -  phosphoadenosine phosphosulfate reductase;...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2757, JW2732
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Disease relevance of cysH


High impact information on cysH

  • The ts mutation was mapped to 60.6 min of the S. typhimurium chromosome and was linked to argA and cysH [5].
  • Molecular weights deduced for the cysI-encoded sulfite reductase hemoprotein and for the cysH-encoded 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase were approximately 64,000 and 28,000, respectively [1].
  • In order to determine the specificity of the R. meliloti reductase, the R. meliloti cysH homolog was histidine tagged and purified, and its specificity was assayed in vitro [6].
  • Expression of lacZ fused to the cysH promoter was repressed by cysteine and sulfide and induced by sulfur limitation, indicating that cysH is controlled at the level of transcription [4].
  • Both thioredoxin and glutaredoxin have been shown to be hydrogen donors for PAPS reductase (cysH) in vitro (M. L.-S. Tsang, J. Bacteriol. 146:1059-1066, 1981), and one or the other of these compounds is presumably essential in vivo for growth on minimal medium containing sulfate as the sulfur source [7].

Chemical compound and disease context of cysH

  • Involvement of the only Cys and of Tyr209 in the reduction of PAdoPS to sulfite was investigated by site-specific mutagenesis: cysH was mutated by single-strand-overlay extension PCR; the mutated genes were cloned in pBTac1 and expressed in E. coli RL 22 (delta cysHIJ) [8].

Biological context of cysH

  • The open reading frame was 735 nucleotides in length; it was flanked by a repetitive palindromic sequence centred 36 nucleotides upstream of cysH and a terminator-like structure located 20 nucleotides downstream [2].

Associations of cysH with chemical compounds

  • The structural gene (cysH) encodes for a small polypeptide with a single Cys residue located in a conserved cluster (KXECGI/LH) of amino acids [8].


  1. Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase. Ostrowski, J., Wu, J.Y., Rueger, D.C., Miller, B.E., Siegel, L.M., Kredich, N.M. J. Biol. Chem. (1989) [Pubmed]
  2. Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli. Krone, F.A., Westphal, G., Schwenn, J.D. Mol. Gen. Genet. (1991) [Pubmed]
  3. Structure and function of a cysBJIH gene cluster in the purple sulphur bacterium Thiocapsa roseopersicina. Haverkamp, T., Schwenn, J.D. Microbiology (Reading, Engl.) (1999) [Pubmed]
  4. L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase. Mansilla, M.C., de Mendoza, D. J. Bacteriol. (1997) [Pubmed]
  5. Isolation and characterization of a temperature-sensitive mutant of Salmonella typhimurium defective in prolipoprotein modification. Gan, K., Gupta, S.D., Sankaran, K., Schmid, M.B., Wu, H.C. J. Biol. Chem. (1993) [Pubmed]
  6. Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae. Abola, A.P., Willits, M.G., Wang, R.C., Long, S.R. J. Bacteriol. (1999) [Pubmed]
  7. Thioredoxin or glutaredoxin in Escherichia coli is essential for sulfate reduction but not for deoxyribonucleotide synthesis. Russel, M., Model, P., Holmgren, A. J. Bacteriol. (1990) [Pubmed]
  8. Reaction mechanism of thioredoxin: 3'-phospho-adenylylsulfate reductase investigated by site-directed mutagenesis. Berendt, U., Haverkamp, T., Prior, A., Schwenn, J.D. Eur. J. Biochem. (1995) [Pubmed]
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