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Gene Review

purA  -  adenylosuccinate synthetase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4173, JW4135
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Disease relevance of purA


High impact information on purA


Chemical compound and disease context of purA


Biological context of purA


Anatomical context of purA


Associations of purA with chemical compounds

  • Introduction of a purR mutation in wild-type and pur-lac fusion strains was found to abolish purine repression of all genes of the purine biosynthetic pathway except for purA [16].
  • In purA mutants deprived of adenine, this amount increased sixfold within 2 h [17].
  • The cloned E. ictaluri purA gene was mutated by deleting a 598-bp segment of the gene and inserting the kanamycin resistance gene from Tn903 into the gap [3].
  • The three substrates of adenylosuccinate synthetase (GTP, IMP, and aspartate) showed different effects in their ability to protect the enzyme against PLP inactivation [10].
  • These findings suggest that the glycine-rich sequence in adenylosuccinate synthetase functions differently relative to those in other GTP binding proteins or enzymes [11].

Other interactions of purA


Analytical, diagnostic and therapeutic context of purA


  1. Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12. Wolfe, S.A., Smith, J.M. J. Biol. Chem. (1988) [Pubmed]
  2. Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP. Mäntsälä, P., Zalkin, H. J. Bacteriol. (1992) [Pubmed]
  3. Attenuation, persistence, and vaccine potential of an Edwardsiella ictaluri purA mutant. Lawrence, M.L., Cooper, R.K., Thune, R.L. Infect. Immun. (1997) [Pubmed]
  4. The Escherichia coli transcriptional regulator MarA directly represses transcription of purA and hdeA. Schneiders, T., Barbosa, T.M., McMurry, L.M., Levy, S.B. J. Biol. Chem. (2004) [Pubmed]
  5. Variations in the response of mouse isozymes of adenylosuccinate synthetase to inhibitors of physiological relevance. Borza, T., Iancu, C.V., Pike, E., Honzatko, R.B., Fromm, H.J. J. Biol. Chem. (2003) [Pubmed]
  6. IMP Alone Organizes the Active Site of Adenylosuccinate Synthetase from Escherichia coli. Hou, Z., Wang, W., Fromm, H.J., Honzatko, R.B. J. Biol. Chem. (2002) [Pubmed]
  7. Determinants of L-aspartate and IMP recognition in Escherichia coli adenylosuccinate synthetase. Gorrell, A., Wang, W., Underbakke, E., Hou, Z., Honzatko, R.B., Fromm, H.J. J. Biol. Chem. (2002) [Pubmed]
  8. Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase. Hou, Z., Cashel, M., Fromm, H.J., Honzatko, R.B. J. Biol. Chem. (1999) [Pubmed]
  9. Evidence for an arginine residue at the substrate binding site of Escherichia coli adenylosuccinate synthetase as studied by chemical modification and site-directed mutagenesis. Dong, Q., Liu, F., Myers, A.M., Fromm, H.J. J. Biol. Chem. (1991) [Pubmed]
  10. Chemical modification of adenylosuccinate synthetase from Escherichia coli by pyridoxal 5'-phosphate. Identification of an active site lysyl residue. Dong, Q., Fromm, H.J. J. Biol. Chem. (1990) [Pubmed]
  11. Site-directed mutagenesis of the phosphate-binding consensus sequence in Escherichia coli adenylosuccinate synthetase. Liu, F., Dong, Q., Fromm, H.J. J. Biol. Chem. (1992) [Pubmed]
  12. Crystal structures of adenylosuccinate synthetase from Escherichia coli complexed with GDP, IMP hadacidin, NO3-, and Mg2+. Poland, B.W., Fromm, H.J., Honzatko, R.B. J. Mol. Biol. (1996) [Pubmed]
  13. Autoregulation of the dnaA-dnaN operon and effects of DnaA protein levels on replication initiation in Bacillus subtilis. Ogura, Y., Imai, Y., Ogasawara, N., Moriya, S. J. Bacteriol. (2001) [Pubmed]
  14. vacB, a novel chromosomal gene required for expression of virulence genes on the large plasmid of Shigella flexneri. Tobe, T., Sasakawa, C., Okada, N., Honma, Y., Yoshikawa, M. J. Bacteriol. (1992) [Pubmed]
  15. Escherichia coli K1 purA and sorC are preferentially expressed upon association with human brain microvascular endothelial cells. Hoffman, J.A., Badger, J.L., Zhang, Y., Kim, K.S. Microb. Pathog. (2001) [Pubmed]
  16. Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli. Meng, L.M., Kilstrup, M., Nygaard, P. Eur. J. Biochem. (1990) [Pubmed]
  17. Regulation of photolyase in Escherichia coli K-12 during adenine deprivation. Alcorn, J.L., Rupert, C.S. J. Bacteriol. (1990) [Pubmed]
  18. Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene. Clyman, J., Cunningham, R.P. J. Bacteriol. (1991) [Pubmed]
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