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Gene Review

ENTPD3  -  ectonucleoside triphosphate...

Homo sapiens

Synonyms: CD39 antigen-like 3, CD39L3, Ecto-ATP diphosphohydrolase 3, Ecto-ATPDase 3, Ecto-ATPase 3, ...
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High impact information on ENTPD3

  • On desialylated JOK-1 cells, ecto-ST in the presence of exogenous CMP-NeuAc was able to resialylate the B-cell surface sialoglycans CDw75 and HB-6 and major surface glycoproteins of B cells, such as HLA class I and II antigens, transferrin receptor, and surface IgM [1].
  • Characterization of disulfide bonds in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): implications for NTPDase structural modeling [2].
  • To investigate disulfide structure in human NTPDase3, we made single and double mutants of these 10 cysteines, and analyzed their enzymatic activity, glycosylation pattern, trafficking to the cell membrane, and sensitivity to reduction [2].
  • METHODS: Platelet aggregation in the presence of HUVEC and endothelial surface expression and activities of ecto-ATP diphosphohydrolase (ecto-ADPase), CD39, were determined [3].
  • Ecto-nucleoside triphosphate diphosphohydrolase 3 (eNTPDase-3, also known as HB6 and CD39L3) is a membrane-associated ecto-apyrase [4].

Biological context of ENTPD3


Anatomical context of ENTPD3


Associations of ENTPD3 with chemical compounds

  • Mutation of tryptophan 187 to alanine yielded a poorly expressed ecto-apyrase completely devoid of nucleotidase activity [6].
  • Only one of these putative glycosylation sites, asparagine 81 in NTPDase3, which is located near apyrase conserved region 1 (ACR1), is invariant in all the cell surface membrane eNTPDases [10].
  • Metabolism of extracellular ATP to adenosine required PMNs, and studies addressing these metabolic steps revealed that PMN express surface ecto-apyrase (CD39) [11].

Other interactions of ENTPD3

  • Tenocytes (TSC and TIF) expressed ecto-nucleotidase mRNA (ENTPD3 and ENPP1, ENPP2) [12].
  • The most potent compound was K(6)H(2)[TiW(11)CoO(40)], exhibiting K(i) values of 0.140muM (NTPDase1), 0.910muM (NTPDase2), and 0.563muM (NTPDase3) [13].

Analytical, diagnostic and therapeutic context of ENTPD3

  • A human brain E-type ATPase (HB6 ecto-apyrase) was subjected to site-directed mutagenesis to assess the functional significance of two highly conserved tryptophan residues (Trp 187 and Trp 459), the only two tryptophans conserved in nearly all E-type ATPases [6].
  • In addition, the loss of TFPI and vascular ATPDase/CD39 activity following EC activation responses would potentiate any procoagulant changes within the xenograft [14].


  1. Ecto-sialyltransferase of human B lymphocytes reconstitutes differentiation markers in the presence of exogenous CMP-N-acetyl neuraminic acid. Gross, H.J., Merling, A., Moldenhauer, G., Schwartz-Albiez, R. Blood (1996) [Pubmed]
  2. Characterization of disulfide bonds in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): implications for NTPDase structural modeling. Ivanenkov, V.V., Meller, J., Kirley, T.L. Biochemistry (2005) [Pubmed]
  3. Perindopril augments ecto-ATP diphosphohydrolase activity and enhances endothelial anti-platelet function in human umbilical vein endothelial cells. Kishi, Y., Ohta, S., Kasuya, N., Sakita, S.Y., Ashikaga, T., Isobe, M. J. Hypertens. (2003) [Pubmed]
  4. Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions. Yang, F., Hicks-Berger, C.A., Smith, T.M., Kirley, T.L. Biochemistry (2001) [Pubmed]
  5. The CD39-like gene family: identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster. Chadwick, B.P., Frischauf, A.M. Genomics (1998) [Pubmed]
  6. Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase. Smith, T.M., Lewis Carl, S.A., Kirley, T.L. Biochemistry (1999) [Pubmed]
  7. Cloning and mapping of a human and mouse gene with homology to ecto-ATPase genes. Chadwick, B.P., Frischauf, A.M. Mamm. Genome (1997) [Pubmed]
  8. Analysis of CD39/ATP diphosphohydrolase (ATPDase) expression in endothelial cells, platelets and leukocytes. Koziak, K., Sévigny, J., Robson, S.C., Siegel, J.B., Kaczmarek, E. Thromb. Haemost. (1999) [Pubmed]
  9. Noise-induced up-regulation of NTPDase3 expression in the rat cochlea: Implications for auditory transmission and cochlear protection. Vlajkovic, S.M., Vinayagamoorthy, A., Thorne, P.R., Robson, S.C., Wang, C.J., Housley, G.D. Brain Res. (2006) [Pubmed]
  10. Asparagine 81, an invariant glycosylation site near apyrase conserved region 1, is essential for full enzymatic activity of ecto-nucleoside triphosphate diphosphohydrolase 3. Murphy, D.M., Kirley, T.L. Arch. Biochem. Biophys. (2003) [Pubmed]
  11. ATP release from activated neutrophils occurs via connexin 43 and modulates adenosine-dependent endothelial cell function. Eltzschig, H.K., Eckle, T., Mager, A., K??per, N., Karcher, C., Weissm??ller, T., Boengler, K., Schulz, R., Robson, S.C., Colgan, S.P. Circ. Res. (2006) [Pubmed]
  12. Mechanical loading stimulates ecto-ATPase activity in human tendon cells. Tsuzaki, M., Bynum, D., Almekinders, L., Faber, J., Banes, A.J. J. Cell. Biochem. (2005) [Pubmed]
  13. Polyoxometalates-a new class of potent ecto-nucleoside triphosphate diphosphohydrolase (NTPDase) inhibitors. M??ller, C.E., Iqbal, J., Baqi, Y., Zimmermann, H., R??llich, A., Stephan, H. Bioorg. Med. Chem. Lett. (2006) [Pubmed]
  14. Factors in xenograft rejection. Robson, S.C., Schulte am Esch, J., Bach, F.H. Ann. N. Y. Acad. Sci. (1999) [Pubmed]
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