Disease relevance of PSCDBP

• But P. radiora RP-C, P. fluorescens B3-1, E. coli B/r and E. coli K-12 were hardly sensitized by N2O [1].

High impact information on PSCDBP

• Acceleration of guanosine 5prime prime or minute-O-(thiotriphosphate) binding to ARF by cytohesin-1 in vitro was enhanced by Cybr [2].
• However, competitive stem cell repopulation experiments showed a defect in the abilities of Cybr-deficient T cells to develop in the presence of wild-type precursors [3].
• Cytohesin binder and regulator (cybr) is not essential for T- and dendritic-cell activation and differentiation [3].
• These data suggest that Cybr is not absolutely required for hematopoietic cell development or function, but stem cells lacking Cybr are at a developmental disadvantage compared to wild-type cells [3].
• Despite the selective expression in hematopoietic cells, there was no intrinsic defect in T- or B-cell development or function in Cybr-deficient mice [3].

Biological context of PSCDBP

• Assignment of the human B3-1 gene (PSCDBP) to chromosome 2 band q11.2 by radiation hybrid mapping [4].
• The effects of Cybr on nuclear factor of activated T cells and AP-1 are dependent on MAPK activation, and enhanced activation of this cascade results in cooperation between the two transcription factors in the regulation of gene expression [5].
• A 1724 base pair (bp) clone, B3-1, was obtained from a human NK subtracted cDNA library and sequenced [6].
• METHOD: A sample of 12 research papers on support for breast-feeding was appraised by six qualitative reviewers using three appraisal methods: unprompted judgement, based on expert opinion; a UK Cabinet Office quality framework; and CASP, a Critical Appraisal Skills Programme tool [7].

Anatomical context of PSCDBP

• Yeast two-hybrid screening of a B cell library using the cytohesin N terminus as bait, identified CASP, a scaffolding protein of previously unknown function, as a binding partner [8].
• CASP contains an internal coiled coil motif that is required for cytohesin binding both in vitro and in COS-1 cells [8].
• Here we show that Cybr mRNA and protein levels are increased upon T cell receptor engagement [5].
• Cybr protein associates with members of the cytohesin family, which are known ADP-ribosylation factors-GDP/GTP exchange factors, and its functions appear to regulate lymphocyte adhesion and cell-cell contact [5].
• All fragments gave rise to at least some colonies, but colony-forming ability was mostly marked in the intermediate part (B2) and the lower half of the central part (B3-1); approximately 60% of colony-forming cells of a hair follicle localized to the fragment B3-1 and 28% to the fragment B3-2 (upper half of the central part, including bulge) [9].

Associations of PSCDBP with chemical compounds

• Dendritic cells regulate T-cell deattachment through the integrin-interacting protein CYTIP [10].

Other interactions of PSCDBP

• Specific silencing of CYTIP results in stronger adhesion of DCs to T cells and to fibronectin [10].

Analytical, diagnostic and therapeutic context of PSCDBP

• In immunofluorescence experiments, CASP localizes to perinuclear tubulovesicular structures that are in close proximity to the Golgi [8].
• CYTIP, which is induced during maturation of DCs, shortly accumulates to the contact zones with T cells within the first hour of coculture [10].
• The adoptive transfer of Cybr-deficient DCs showed that they migrated efficiently and stimulated proliferation and cytokine production by T cells in vivo [3].
• By microdissection, five fragments of the outer root sheath (B1, B2, B3-1, B3-2, B4) were separated, dispersed by trypsin into single cell suspensions, and grown on human feeder fibroblasts [9].
• However, the allele-specific LC PCR with CYBR green 1 method is most rapid, easier to perform, and least expensive technique, and it can be readily performed in most molecular diagnostic laboratories [11].

References