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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
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Disease relevance of Microdissection


High impact information on Microdissection

  • Normal human breast lobules, freshly isolated by precision microdissection of tissue stained with methylene blue chloride, were assayed for their ability to induce neovascularization (angiogenesis) in rabbit irises [6].
  • Similarly, Kupffer cells obtained by laser capture microdissection from the livers of BDL mice expressed increased levels of IL-6 messenger RNA [7].
  • METHODS: One hundred eighty cirrhotic nodules from 7 female patients with HCC were collected by microdissection [8].
  • The animals were killed at 12 days, and plasma, gastrin, and enteroglucagon were measured, while cell proliferation was determined by measuring the crypt cell production rate employing a stathmokinetic method using vincristine and crypt microdissection [9].
  • We collected mRNA for PCR analysis from individual transforming MEE cells by laser microdissection techniques and demonstrated that TGFbeta3 stimulates lymphoid-enhancing factor 1 (LEF1) mRNA synthesis in MEE cells [10].

Chemical compound and disease context of Microdissection


Biological context of Microdissection


Anatomical context of Microdissection


Associations of Microdissection with chemical compounds

  • DNA samples were obtained from formalin-fixed paraffin-embedded sections using tissue microdissection [26].
  • To determine whether the apparent losses of portions of 6q are real, four tumors with 6q deletions were hybridized with a biotin-labeled microdissection probe from 6q24-ter [27].
  • However, a higher level of progestin binding in the VMN of females than of males was found when these brain regions were examined using a microdissection technique [28].
  • Cortical collecting tubules (CCT) were isolated by microdissection and used for measurements of the activity of 11beta-HSD2 by assessing the conversion of corticosterone to dehydrocorticosterone [29].
  • Expression of Ca2+-inhibitable types V and VI adenylyl cyclases was studied by reverse transcription-polymerase chain reaction in rat renal glomeruli and nephron segments isolated by microdissection [30].

Gene context of Microdissection


Analytical, diagnostic and therapeutic context of Microdissection

  • Quantitative real-time RT-PCR aided by laser microdissection indicated that the expression of representative EARLY genes (Sgk, IkBa, and Plekhf1) peaked at 1-d training in both the paired and unpaired conditioning groups, and was maintained at a higher level in the paired group than in the unpaired group after 3-d training [36].
  • Western blots of tissue derived from microdissection of the surface and core regions of the olfactory bulb were performed using antibodies to synapsin I and synapsin II [37].
  • In conclusion, microdissection genotyping of HCC is an effective and objective means to (1) distinguish between de novo HCC tumor formation versus intrahepatic spread of cancer and to (2) improve on current methods for prediction of tumor aggressiveness and recurrence-free survival after liver transplantation [38].
  • Furthermore, it was shown that formalin fixation as well as hematoxylin staining of frozen sections with Delafield's and Mayer's alum or Weigert's iron hematoxylin before microdissection significantly diminishes the amount of extractable DNA and may lead to less reliable results, even of qualitative PCR analysis [39].
  • Ultra-low temperature techniques (microdissection and autoradiography) were used to study the nucleocytoplasmic distribution and transport of alpha-aminoisobutyric acid (AIB) in an amino acid-accumulating cell [40].


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  2. Identification and validation of P311 as a glioblastoma invasion gene using laser capture microdissection. Mariani, L., McDonough, W.S., Hoelzinger, D.B., Beaudry, C., Kaczmarek, E., Coons, S.W., Giese, A., Moghaddam, M., Seiler, R.W., Berens, M.E. Cancer Res. (2001) [Pubmed]
  3. Allelic deletions on chromosome 11q13 in multiple endocrine neoplasia type 1-associated and sporadic gastrinomas and pancreatic endocrine tumors. Debelenko, L.V., Zhuang, Z., Emmert-Buck, M.R., Chandrasekharappa, S.C., Manickam, P., Guru, S.C., Marx, S.J., Skarulis, M.C., Spiegel, A.M., Collins, F.S., Jensen, R.T., Liotta, L.A., Lubensky, I.A. Cancer Res. (1997) [Pubmed]
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  5. Molecular genetic evidence for different clonal origins of epithelial and stromal components of phyllodes tumor of the prostate. McCarthy, R.P., Zhang, S., Bostwick, D.G., Qian, J., Eble, J.N., Wang, M., Lin, H., Cheng, L. Am. J. Pathol. (2004) [Pubmed]
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  7. Kupffer cells abrogate cholestatic liver injury in mice. Gehring, S., Dickson, E.M., San Martin, M.E., van Rooijen, N., Papa, E.F., Harty, M.W., Tracy, T.F., Gregory, S.H. Gastroenterology (2006) [Pubmed]
  8. Chromosomal allelic imbalance evolving from liver cirrhosis to hepatocellular carcinoma. Yeh, S.H., Chen, P.J., Shau, W.Y., Chen, Y.W., Lee, P.H., Chen, J.T., Chen, D.S. Gastroenterology (2001) [Pubmed]
  9. Evidence for a humoral mechanism after small intestinal resection. Exclusion of gastrin but not enteroglucagon. Sagor, G.R., Ghatei, M.A., Al-Mukhtar, M.Y., Wright, N.A., Bloom, S.R. Gastroenterology (1983) [Pubmed]
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  11. The effect of clonidine on tubular obstruction in postischemic acute renal failure in the rabbit demonstrated by microradiography and microdissection. Ideura, T., Solez, K., Heptinstall, R.H. Am. J. Pathol. (1980) [Pubmed]
  12. Somatic mutations of epidermal growth factor receptor in bile duct and gallbladder carcinoma. Leone, F., Cavalloni, G., Pignochino, Y., Sarotto, I., Ferraris, R., Piacibello, W., Venesio, T., Capussotti, L., Risio, M., Aglietta, M. Clin. Cancer Res. (2006) [Pubmed]
  13. Increase of hypothalamic cholinergic activity in 2-deoxyglucose hyperglycemia. Takahashi, A., Ishimaru, H., Ikarashi, Y., Kishi, E., Maruyama, Y. Neurosci. Lett. (1994) [Pubmed]
  14. Chronic hyperprolactinemia and changes in dopamine neurons. Mohankumar, P.S., Mohankumar, S.M., Quadri, S.K., Voogt, J.L. Brain Res. Bull. (1997) [Pubmed]
  15. Serial follow-up study of serum testosterone and antisperm antibodies in patients with non-obstructive azoospermia after conventional or microdissection testicular sperm extraction. Komori, K., Tsujimura, A., Miura, H., Shin, M., Takada, T., Honda, M., Matsumiya, K., Fujioka, H. Int. J. Androl. (2004) [Pubmed]
  16. Band-specific localization of the microsatellite at D13S71 by microdissection and enzymatic amplification. Spielvogel, H., Hennies, H.C., Claussen, U., Washington, S.S., Chakravarti, A., Reis, A. Am. J. Hum. Genet. (1992) [Pubmed]
  17. High frequency of epithelial chimerism in liver transplants demonstrated by microdissection and STR-analysis. Kleeberger, W., Rothämel, T., Glöckner, S., Flemming, P., Lehmann, U., Kreipe, H. Hepatology (2002) [Pubmed]
  18. The use of specific DNA probes to analyse the Sxr mutation in the mouse. Bishop, C.E., Roberts, C., Michot, J.L., Nagamine, C., Winking, H., Guénet, J.L., Weith, A. Development (1987) [Pubmed]
  19. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Specht, K., Richter, T., Müller, U., Walch, A., Werner, M., Höfler, H. Am. J. Pathol. (2001) [Pubmed]
  20. Constitutive expression and modulation of the functional thrombin receptor in the human kidney. Xu, Y., Zacharias, U., Peraldi, M.N., He, C.J., Lu, C., Sraer, J.D., Brass, L.F., Rondeau, E. Am. J. Pathol. (1995) [Pubmed]
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  22. Expression of insulin-like growth factor I in developing lens is compartmentalized. Caldés, T., Alemany, J., Robcis, H.L., de Pablo, F. J. Biol. Chem. (1991) [Pubmed]
  23. A new method for the microdissection of the human hypothalamus, with mapping of cholinergic, GABA and catecholamine systems in twelve nuclei and areas. Langevin, H., Iversen, L.L. Brain (1980) [Pubmed]
  24. Hypermethylation of the human glutathione S-transferase-pi gene (GSTP1) CpG island is present in a subset of proliferative inflammatory atrophy lesions but not in normal or hyperplastic epithelium of the prostate: a detailed study using laser-capture microdissection. Nakayama, M., Bennett, C.J., Hicks, J.L., Epstein, J.I., Platz, E.A., Nelson, W.G., De Marzo, A.M. Am. J. Pathol. (2003) [Pubmed]
  25. Mutations of the BRAF gene in squamous cell carcinoma of the head and neck. Weber, A., Langhanki, L., Sommerer, F., Markwarth, A., Wittekind, C., Tannapfel, A. Oncogene (2003) [Pubmed]
  26. Atypical adenomatous hyperplasia of the prostate: a premalignant lesion? Cheng, L., Shan, A., Cheville, J.C., Qian, J., Bostwick, D.G. Cancer Res. (1998) [Pubmed]
  27. Consistent chromosome abnormalities in adenocarcinoma of the pancreas. Griffin, C.A., Hruban, R.H., Morsberger, L.A., Ellingham, T., Long, P.P., Jaffee, E.M., Hauda, K.M., Bohlander, S.K., Yeo, C.J. Cancer Res. (1995) [Pubmed]
  28. Regional sex differences in progestin receptor induction in the rat hypothalamus: effects of various doses of estradiol benzoate. Brown, T.J., Clark, A.S., MacLusky, N.J. J. Neurosci. (1987) [Pubmed]
  29. Inhibition of 11beta-hydroxysteroid dehydrogenase by bile acids in rats with cirrhosis. Ackermann, D., Vogt, B., Escher, G., Dick, B., Reichen, J., Frey, B.M., Frey, F.J. Hepatology (1999) [Pubmed]
  30. Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content. Chabardès, D., Firsov, D., Aarab, L., Clabecq, A., Bellanger, A.C., Siaume-Perez, S., Elalouf, J.M. J. Biol. Chem. (1996) [Pubmed]
  31. Estrogen sulfotransferase and steroid sulfatase in human breast carcinoma. Suzuki, T., Nakata, T., Miki, Y., Kaneko, C., Moriya, T., Ishida, T., Akinaga, S., Hirakawa, H., Kimura, M., Sasano, H. Cancer Res. (2003) [Pubmed]
  32. Role of discoidin domain receptors 1 and 2 in human smooth muscle cell-mediated collagen remodeling: potential implications in atherosclerosis and lymphangioleiomyomatosis. Ferri, N., Carragher, N.O., Raines, E.W. Am. J. Pathol. (2004) [Pubmed]
  33. Renal angiomyolipomas from patients with sporadic lymphangiomyomatosis contain both neoplastic and non-neoplastic vascular structures. Karbowniczek, M., Yu, J., Henske, E.P. Am. J. Pathol. (2003) [Pubmed]
  34. Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor. Munnia, A., Schütz, N., Romeike, B.F., Maldener, E., Glass, B., Maas, R., Nastainczyk, W., Feiden, W., Fischer, U., Meese, E. Oncogene (2001) [Pubmed]
  35. SOCS-3 is frequently methylated in head and neck squamous cell carcinoma and its precursor lesions and causes growth inhibition. Weber, A., Hengge, U.R., Bardenheuer, W., Tischoff, I., Sommerer, F., Markwarth, A., Dietz, A., Wittekind, C., Tannapfel, A. Oncogene (2005) [Pubmed]
  36. Molecular evidence for two-stage learning and partial laterality in eyeblink conditioning of mice. Park, J.S., Onodera, T., Nishimura, S., Thompson, R.F., Itohara, S. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  37. Differential distribution of the synapsins in the rat olfactory bulb. Stone, L.M., Browning, M.D., Finger, T.E. J. Neurosci. (1994) [Pubmed]
  38. Microdissection-based allelotyping discriminates de novo tumor from intrahepatic spread in hepatocellular carcinoma. Finkelstein, S.D., Marsh, W., Demetris, A.J., Swalsky, P.A., Sasatomi, E., Bonham, A., Subotin, M., Dvorchik, I. Hepatology (2003) [Pubmed]
  39. Quantitation of DNA extracted after micropreparation of cells from frozen and formalin-fixed tissue sections. Serth, J., Kuczyk, M.A., Paeslack, U., Lichtinghagen, R., Jonas, U. Am. J. Pathol. (2000) [Pubmed]
  40. Nucleocytoplasmic transport and distribution of an amino acid, in situ. Frank, M., Horowitz, S.B. J. Cell. Sci. (1975) [Pubmed]
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