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MeSH Review

Microdissection

 
 
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Disease relevance of Microdissection

 

High impact information on Microdissection

  • Normal human breast lobules, freshly isolated by precision microdissection of tissue stained with methylene blue chloride, were assayed for their ability to induce neovascularization (angiogenesis) in rabbit irises [6].
  • Similarly, Kupffer cells obtained by laser capture microdissection from the livers of BDL mice expressed increased levels of IL-6 messenger RNA [7].
  • METHODS: One hundred eighty cirrhotic nodules from 7 female patients with HCC were collected by microdissection [8].
  • The animals were killed at 12 days, and plasma, gastrin, and enteroglucagon were measured, while cell proliferation was determined by measuring the crypt cell production rate employing a stathmokinetic method using vincristine and crypt microdissection [9].
  • We collected mRNA for PCR analysis from individual transforming MEE cells by laser microdissection techniques and demonstrated that TGFbeta3 stimulates lymphoid-enhancing factor 1 (LEF1) mRNA synthesis in MEE cells [10].
 

Chemical compound and disease context of Microdissection

 

Biological context of Microdissection

 

Anatomical context of Microdissection

 

Associations of Microdissection with chemical compounds

  • DNA samples were obtained from formalin-fixed paraffin-embedded sections using tissue microdissection [26].
  • To determine whether the apparent losses of portions of 6q are real, four tumors with 6q deletions were hybridized with a biotin-labeled microdissection probe from 6q24-ter [27].
  • However, a higher level of progestin binding in the VMN of females than of males was found when these brain regions were examined using a microdissection technique [28].
  • Cortical collecting tubules (CCT) were isolated by microdissection and used for measurements of the activity of 11beta-HSD2 by assessing the conversion of corticosterone to dehydrocorticosterone [29].
  • Expression of Ca2+-inhibitable types V and VI adenylyl cyclases was studied by reverse transcription-polymerase chain reaction in rat renal glomeruli and nephron segments isolated by microdissection [30].
 

Gene context of Microdissection

 

Analytical, diagnostic and therapeutic context of Microdissection

  • Quantitative real-time RT-PCR aided by laser microdissection indicated that the expression of representative EARLY genes (Sgk, IkBa, and Plekhf1) peaked at 1-d training in both the paired and unpaired conditioning groups, and was maintained at a higher level in the paired group than in the unpaired group after 3-d training [36].
  • Western blots of tissue derived from microdissection of the surface and core regions of the olfactory bulb were performed using antibodies to synapsin I and synapsin II [37].
  • In conclusion, microdissection genotyping of HCC is an effective and objective means to (1) distinguish between de novo HCC tumor formation versus intrahepatic spread of cancer and to (2) improve on current methods for prediction of tumor aggressiveness and recurrence-free survival after liver transplantation [38].
  • Furthermore, it was shown that formalin fixation as well as hematoxylin staining of frozen sections with Delafield's and Mayer's alum or Weigert's iron hematoxylin before microdissection significantly diminishes the amount of extractable DNA and may lead to less reliable results, even of qualitative PCR analysis [39].
  • Ultra-low temperature techniques (microdissection and autoradiography) were used to study the nucleocytoplasmic distribution and transport of alpha-aminoisobutyric acid (AIB) in an amino acid-accumulating cell [40].

References

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  19. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Specht, K., Richter, T., Müller, U., Walch, A., Werner, M., Höfler, H. Am. J. Pathol. (2001) [Pubmed]
  20. Constitutive expression and modulation of the functional thrombin receptor in the human kidney. Xu, Y., Zacharias, U., Peraldi, M.N., He, C.J., Lu, C., Sraer, J.D., Brass, L.F., Rondeau, E. Am. J. Pathol. (1995) [Pubmed]
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  27. Consistent chromosome abnormalities in adenocarcinoma of the pancreas. Griffin, C.A., Hruban, R.H., Morsberger, L.A., Ellingham, T., Long, P.P., Jaffee, E.M., Hauda, K.M., Bohlander, S.K., Yeo, C.J. Cancer Res. (1995) [Pubmed]
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