Disease relevance of Isodialuric acid

• Relative importance of cellular uptake and reactive oxygen species for the toxicity of alloxan and dialuric acid to insulin-producing cells [1].
• The cells were then washed and subjected to the dialuric acid hemolysis test [2].
• We describe a novel activity of E. coli uracil DNA N-glycosylase (UNG) that excises isodialuric acid from DNA [3].

High impact information on Isodialuric acid

• The toxic action of alloxan on animal cells, particularly the beta cells of pancreas, may be caused by rapid oxidation of cellular NADPH and generation of cytotoxic dialuric acid catalyzed by the thioredoxin system [4].
• After a second attack by another .OH, the BA radical is transformed into dialuric acid (DA), which autoxidizes via the alloxan radical (.ALX) to ALX [5].
• Alloxan and its reduced derivative, dialuric acid, act as a redox couple, driven by reduced glutathione (GSH) or L-cysteine, generating in vitro in the presence of oxygen, both superoxide radical and hydrogen peroxide [6].
• The peroxidation of membrane phospholipids induced in vitro by ascorbic acid or by dialuric acid (hydroxybarbituric acid) does not occur in the absence of traces of metal ions [7].
• The oxidation of these substrates by Br2 led to a similar profile of intermediate and stable products indicating that the dialuric and isodialuric acid derivatives of dCyd largely undergo deamination before they transform into 5-oh-dHyd [8].

Chemical compound and disease context of Isodialuric acid

• Degree of erythrocyte hemolysis induced by dialuric acid, level of plasma vitamin E and extent of liver lipid peroxidation were determined [9].
• A novel activity of E. coli uracil DNA N-glycosylase excision of isodialuric acid (5,6-dihydroxyuracil), a major product of oxidative DNA damage, from DNA [3].
• The transfer of alpha-tocopherol from plasma to erythrocytes was examined using labeled alpha-tocopherol in vitamin E-deficient rats and at the same time the appearance of resistance to dialuric acid-induced hemolysis in the erythrocytes was observed [10].
• The plasma and red blood cell (RBC) levels of alpha-tocopherol in rats were determined in association with hemolysis induced by dialuric acid for a 7-week period of tocopherol deprivation following a 2-week period on tocopherol-containing diet [11].

Biological context of Isodialuric acid

• Hydroxylation was inhibited by catalase but not by superoxide dismutase, and it is attributed to the Fenton reaction in which the ferric catalyst is reduced by dialuric acid [12].
• The desolvated crystals of dialuric acid monohydrate had approximately the same crystal structure as the monohydrate and were stable in air at room temperature at low humidities [13].
• Formation of isodialuric acid lesion within DNA oligomers via one-electron oxidation of 5-hydroxyuracil: characterization, stability and excision repair [14].

Anatomical context of Isodialuric acid

• After 3 weeks, their erythrocytes were 96.9 +/- 0.6% hemolyzed by dialuric acid [15].
• Again, dialuric acid and BuOOH were less potent inhibitors while ninhydrin and NEM were more potent inhibitors of aconitase in intact liver cells [16].

Associations of Isodialuric acid with other chemical compounds

• Release of iron from ferritin by divicine, isouramil, acid-hydrolyzed vicine, and dialuric acid and initiation of lipid peroxidation [17].
• Of the four tests, dialuric acid haemolysis was the most sensitive in-vitro assay for vitamin E deficiency in adult dogs [18].
• Simultaneous quantitative determination of alloxan, GSH and GSSG by HPlc. Estimation of the frequency of redox cycling between alloxan and dialuric acid [19].
• 4. The combination of vanadium ions and alloxan-GSH or dialuric acid had no additive effect on lipid peroxidation [20].

Gene context of Isodialuric acid

• Observations on the mechanism of the oxygen/dialuric acid-induced hemolysis of vitamin e-deficient rat red blood cells and the protective roles of catalase and superoxide dismutase [21].
• Vanadium ions decreased the dialuric acid-induced inhibition of catalase activity [20].

Analytical, diagnostic and therapeutic context of Isodialuric acid

• Following precipitation of DNA, analyses of pellets and supernatant fractions by gas chromatography/mass spectrometry showed an efficient excision of isodialuric acid from DNA by UNG [3].
• Oral administration of vitamin E brought about no increase in serum levels of the vitamin, but hemolysis of erythrocytes in dialuric acid decreased from 18.6 +/- 15.8% to 1.0 +/- 0.03% [22].

References