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Gene Review

NXF1  -  nuclear RNA export factor 1

Homo sapiens

Synonyms: DKFZp667O0311, MEX67, Mex67, Nuclear RNA export factor 1, TAP, ...
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Disease relevance of NXF1


Psychiatry related information on NXF1


High impact information on NXF1

  • Export of mRNAs is mediated by a conserved heterodimeric transport receptor (known as NXF1-p15 in metazoa) that binds to mRNA cargoes either directly or indirectly by means of adaptor proteins [6].
  • Thus, TAP, like its yeast homolog Mex67p, is a bona fide mRNA nuclear export mediator [7].
  • The bimolecular fluorescence complementation (BiFC) assay, which allows the investigation of interacting molecules in vivo, was applied to study complex formation between the splicing factor Y14 and nuclear export factor 1 (NXF1), which evidence indicates are functionally associated with nuclear mRNA [8].
  • Y14 linked to the COOH terminus of yellow fluorescent protein (YFP; YC-Y14), and NXF1 fused to the NH2 terminus of YFP (YN-NXF1) expressed in MCF7 cells yielded BiFC upon specific binding [8].
  • Unlike the NXF1-mediated export of bulk mRNAs, eIF4E-dependent mRNA export is CRM1 dependent [9].

Biological context of NXF1


Anatomical context of NXF1


Associations of NXF1 with chemical compounds

  • The C terminus of ICP27 is required; however, the N-terminal leucine-rich region also contributes to the interaction of ICP27 with TAP/NXF1 [1].

Physical interactions of NXF1

  • Metazoan NXF1/p15 heterodimers promote export of bulk mRNA through nuclear pore complexes (NPC) [18].
  • Y14 is part of a multi-protein complex that also contains the mRNA export factor TAP [19].
  • We also found that RBM15 binds to NXF1 and the two proteins cooperate in stimulating RTE-mediated mRNA export and expression [2].

Other interactions of NXF1

  • Under the same conditions, the steady-state subcellular localization of other nuclear or cytoplasmic proteins and CRM1-mediated protein export are not detectably affected, indicating that the release of NXF1 into the cytoplasm and the inhibition of mRNA export are not due to a general defect in NPC function [12].
  • In RanBP2-depleted cells, NXF1 diffuses freely through the cytoplasm [12].
  • Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP) [20].
  • We propose that RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts [2].
  • Two cellular proteins, RNA helicase A (RHA) and Tip-associated protein (Tap) were identified as binding to CTE and were proposed to function as CTE co-factors (1,2) [21].


  1. ICP27 recruits Aly/REF but not TAP/NXF1 to herpes simplex virus type 1 transcription sites although TAP/NXF1 is required for ICP27 export. Chen, I.H., Li, L., Silva, L., Sandri-Goldin, R.M. J. Virol. (2005) [Pubmed]
  2. RNA-binding Motif Protein 15 Binds to the RNA Transport Element RTE and Provides a Direct Link to the NXF1 Export Pathway. Lindtner, S., Zolotukhin, A.S., Uranishi, H., Bear, J., Kulkarni, V., Smulevitch, S., Samiotaki, M., Panayotou, G., Felber, B.K., Pavlakis, G.N. J. Biol. Chem. (2006) [Pubmed]
  3. An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev protein. Yang, J., Bogerd, H.P., Peng, S., Wiegand, H., Truant, R., Cullen, B.R. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  4. The human endogenous retrovirus K Rev response element coincides with a predicted RNA folding region. Yang, J., Bogerd, H., Le, S.Y., Cullen, B.R. RNA (2000) [Pubmed]
  5. NXF5, a novel member of the nuclear RNA export factor family, is lost in a male patient with a syndromic form of mental retardation. Jun, L., Frints, S., Duhamel, H., Herold, A., Abad-Rodrigues, J., Dotti, C., Izaurralde, E., Marynen, P., Froyen, G. Curr. Biol. (2001) [Pubmed]
  6. The interplay of nuclear mRNP assembly, mRNA surveillance and export. Stutz, F., Izaurralde, E. Trends Cell Biol. (2003) [Pubmed]
  7. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Grüter, P., Tabernero, C., von Kobbe, C., Schmitt, C., Saavedra, C., Bachi, A., Wilm, M., Felber, B.K., Izaurralde, E. Mol. Cell (1998) [Pubmed]
  8. In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains. Schmidt, U., Richter, K., Berger, A.B., Lichter, P. J. Cell Biol. (2006) [Pubmed]
  9. eIF4E is a central node of an RNA regulon that governs cellular proliferation. Culjkovic, B., Topisirovic, I., Skrabanek, L., Ruiz-Gutierrez, M., Borden, K.L. J. Cell Biol. (2006) [Pubmed]
  10. A molecular link between SR protein dephosphorylation and mRNA export. Huang, Y., Yario, T.A., Steitz, J.A. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  11. TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture. Herold, A., Suyama, M., Rodrigues, J.P., Braun, I.C., Kutay, U., Carmo-Fonseca, M., Bork, P., Izaurralde, E. Mol. Cell. Biol. (2000) [Pubmed]
  12. RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export. Forler, D., Rabut, G., Ciccarelli, F.D., Herold, A., Köcher, T., Niggeweg, R., Bork, P., Ellenberg, J., Izaurralde, E. Mol. Cell. Biol. (2004) [Pubmed]
  13. Identification of a novel posttranscriptional regulatory element by using a rev- and RRE-mutated human immunodeficiency virus type 1 DNA proviral clone as a molecular trap. Nappi, F., Schneider, R., Zolotukhin, A., Smulevitch, S., Michalowski, D., Bear, J., Felber, B.K., Pavlakis, G.N. J. Virol. (2001) [Pubmed]
  14. Cotranscriptional recruitment to the mRNA export receptor mex67p contributes to nuclear pore anchoring of activated genes. Dieppois, G., Iglesias, N., Stutz, F. Mol. Cell. Biol. (2006) [Pubmed]
  15. Karyopherin beta 2B participates in mRNA export from the nucleus. Shamsher, M.K., Ploski, J., Radu, A. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  16. RNA association and nucleocytoplasmic shuttling by ataxin-1. Irwin, S., Vandelft, M., Pinchev, D., Howell, J.L., Graczyk, J., Orr, H.T., Truant, R. J. Cell. Sci. (2005) [Pubmed]
  17. Studies on the role of NonA in mRNA biogenesis. Kozlova, N., Braga, J., Lundgren, J., Rino, J., Young, P., Carmo-Fonseca, M., Visa, N. Exp. Cell Res. (2006) [Pubmed]
  18. Nuclear export of mRNA by TAP/NXF1 requires two nucleoporin-binding sites but not p15. Braun, I.C., Herold, A., Rode, M., Izaurralde, E. Mol. Cell. Biol. (2002) [Pubmed]
  19. The Y14 protein communicates to the cytoplasm the position of exon-exon junctions. Kim, V.N., Yong, J., Kataoka, N., Abel, L., Diem, M.D., Dreyfuss, G. EMBO J. (2001) [Pubmed]
  20. Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. Hargous, Y., Hautbergue, G.M., Tintaru, A.M., Skrisovska, L., Golovanov, A.P., Stevenin, J., Lian, L.Y., Wilson, S.A., Allain, F.H. EMBO J. (2006) [Pubmed]
  21. Specific interaction between RNA helicase A and Tap, two cellular proteins that bind to the constitutive transport element of type D retrovirus. Tang, H., Wong-Staal, F. J. Biol. Chem. (2000) [Pubmed]
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