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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


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Disease relevance of Photobleaching


High impact information on Photobleaching

  • Fluorescence recovery after photobleaching of the actin comet tails revealed that endocytic complexes are nucleation sites for rapid actin polymerization [6].
  • Regeneration of rod visual pigments after photobleaching requires a protein called Rpe65 [7].
  • Here we record an intersubunit rotation in real time in the functional enzyme by applying polarized absorption relaxation after photobleaching to immobilized F1 with eosin-labelled gamma [8].
  • We report here a direct determination of the human cone pigment photobleaching difference absorption spectra after the production of each cone pigment apoprotein in tissue culture cells transfected with the corresponding complementary DNA clones [9].
  • The method used extends the FRAP procedure (fluorescence redistribution after photobleaching) to bacteria and the results demonstrate rapid diffusion of lipopolysaccharide (D = 2.0 +/- 0.9 x 10(-10) cm2s-1) over micrometre distances [10].

Chemical compound and disease context of Photobleaching


Biological context of Photobleaching


Anatomical context of Photobleaching


Associations of Photobleaching with chemical compounds

  • Two-photon photobleaching and photorelease of fluorescein dextran were used to generate concentration gradients between spines and shafts in rat CA1 pyramidal neurons [26].
  • In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth [27].
  • Relatively selective removal of bilirubin from the plasma by photobleaching normalized the decreased PMA in five jaundiced patients but produced no apparent change in five normal subjects [28].
  • Fluorescence photobleaching recovery was used to measure the lateral mobility of two integral membrane proteins, glycophorin and band 3, and two lipid analogues, fluorescein phosphatidylethanolamine (Fl-PE) and carbocyanine dyes, in RBCs and ghosts adherent to schistosomula [29].
  • Photobleaching/recovery analysis revealed that the luminal segment determines restricted diffusion and immobility of the protein [30].

Gene context of Photobleaching

  • Photobleaching experiments reveal that MDM2 and VHL are highly mobile proteins in settings where their substrates are efficiently degraded [31].
  • Here, we studied the interaction of Brd4 with chromatin in living cells by photobleaching [32].
  • Photobleaching and tracking of replication foci revealed a dynamic equilibrium between two kinetic pools of PCNA, i.e., bound to replication foci and as a free mobile fraction [33].
  • Fluorescence recovery after photobleaching experiments revealed that Bach2 showed rapid turnover in the nuclear foci [34].
  • Photobleaching experiments revealed that SUMO-1 dynamics was much slower than SUMO-2 and -3 dynamics [35].

Analytical, diagnostic and therapeutic context of Photobleaching


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  2. Iontophoretic delivery of ALA provides a quantitative model for ALA pharmacokinetics and PpIX phototoxicity in human skin. Rhodes, L.E., Tsoukas, M.M., Anderson, R.R., Kollias, N. J. Invest. Dermatol. (1997) [Pubmed]
  3. Lateral diffusion of M-13 coat protein in mixtures of phosphatidylcholine and cholesterol. Smith, L.M., Rubenstein, J.L., Parce, J.W., McConnell, H.M. Biochemistry (1980) [Pubmed]
  4. Photoactive yellow protein from the purple phototrophic bacterium, Ectothiorhodospira halophila. Quantum yield of photobleaching and effects of temperature, alcohols, glycerol, and sucrose on kinetics of photobleaching and recovery. Meyer, T.E., Tollin, G., Hazzard, J.H., Cusanovich, M.A. Biophys. J. (1989) [Pubmed]
  5. Microtubule inhibitors differentially affect translational movement, cell surface expression, and endocytosis of transferrin receptors in K562 cells. Thatte, H.S., Bridges, K.R., Golan, D.E. J. Cell. Physiol. (1994) [Pubmed]
  6. A pathway for association of receptors, adaptors, and actin during endocytic internalization. Kaksonen, M., Sun, Y., Drubin, D.G. Cell (2003) [Pubmed]
  7. The sights along route 65. Saari, J.C. Nat. Genet. (2001) [Pubmed]
  8. Intersubunit rotation in active F-ATPase. Sabbert, D., Engelbrecht, S., Junge, W. Nature (1996) [Pubmed]
  9. Absorption spectra of human cone pigments. Merbs, S.L., Nathans, J. Nature (1992) [Pubmed]
  10. Lateral diffusion of lipopolysaccharide in the outer membrane of Salmonella typhimurium. Schindler, M., Osborn, M.J., Koppel, D.E. Nature (1980) [Pubmed]
  11. Lateral diffusion of proteins in the periplasm of Escherichia coli. Brass, J.M., Higgins, C.F., Foley, M., Rugman, P.A., Birmingham, J., Garland, P.B. J. Bacteriol. (1986) [Pubmed]
  12. Species difference in protoporphyrin IX accumulation produced by an N-phenylimide herbicide in embryos between rats and rabbits. Kawamura, S., Kato, T., Matsuo, M., Katsuda, Y., Yasuda, M. Toxicol. Appl. Pharmacol. (1996) [Pubmed]
  13. L-ascorbic acid quenching of singlet delta molecular oxygen in aqueous media: generalized antioxidant property of vitamin C. Chou, P.T., Khan, A.U. Biochem. Biophys. Res. Commun. (1983) [Pubmed]
  14. Comparison of photodynamic targets in a carcinoma cell line and its mitochondrial DNA-deficient derivative. Morgan, J., Potter, W.R., Oseroff, A.R. Photochem. Photobiol. (2000) [Pubmed]
  15. Photobleaching of astaxanthin and canthaxanthin. Quantum-yields dependence of solvent, temperature, and wavelength of irradiation in relation to packaging and storage of carotenoid pigmented salmonoids. Christophersen, A.G., Jun, H., Jørgensen, K., Skibsted, L.H. Zeitschrift für Lebensmittel-Untersuchung und -Forschung. (1991) [Pubmed]
  16. MAP kinase dynamics in response to pheromones in budding yeast. van Drogen, F., Stucke, V.M., Jorritsma, G., Peter, M. Nat. Cell Biol. (2001) [Pubmed]
  17. A glue for heterochromatin maintenance: stable SUV39H1 binding to heterochromatin is reinforced by the SET domain. Krouwels, I.M., Wiesmeijer, K., Abraham, T.E., Molenaar, C., Verwoerd, N.P., Tanke, H.J., Dirks, R.W. J. Cell Biol. (2005) [Pubmed]
  18. Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. Daigle, N., Beaudouin, J., Hartnell, L., Imreh, G., Hallberg, E., Lippincott-Schwartz, J., Ellenberg, J. J. Cell Biol. (2001) [Pubmed]
  19. Live-cell imaging reveals divergent intracellular dynamics of polyglutamine disease proteins and supports a sequestration model of pathogenesis. Chai, Y., Shao, J., Miller, V.M., Williams, A., Paulson, H.L. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  20. Plasma membrane reorganization induced by tumor promoters in an epithelial cell line. Packard, B.S., Saxton, M.J., Bissell, M.J., Klein, M.P. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  21. VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to agonist-stimulated Ca2+ influx. Singh, B.B., Lockwich, T.P., Bandyopadhyay, B.C., Liu, X., Bollimuntha, S., Brazer, S.C., Combs, C., Das, S., Leenders, A.G., Sheng, Z.H., Knepper, M.A., Ambudkar, S.V., Ambudkar, I.S. Mol. Cell (2004) [Pubmed]
  22. Communication via gap junctions modulates bile secretion in the isolated perfused rat liver. Nathanson, M.H., Rios-Velez, L., Burgstahler, A.D., Mennone, A. Gastroenterology (1999) [Pubmed]
  23. Lateral diffusion in nuclear membranes. Schindler, M., Holland, J.F., Hogan, M. J. Cell Biol. (1985) [Pubmed]
  24. Mobility of fluorescent derivatives of cytochrome c in mitochondria. Vanderkooi, J.M., Maniara, G., Erecinska, M. J. Cell Biol. (1985) [Pubmed]
  25. Steady-state dynamics of Cajal body components in the Xenopus germinal vesicle. Handwerger, K.E., Murphy, C., Gall, J.G. J. Cell Biol. (2003) [Pubmed]
  26. Direct measurement of coupling between dendritic spines and shafts. Svoboda, K., Tank, D.W., Denk, W. Science (1996) [Pubmed]
  27. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Chan, W.C., Nie, S. Science (1998) [Pubmed]
  28. Role of hyperbilirubinemia in the impairment of osteoblast proliferation associated with cholestatic jaundice. Janes, C.H., Dickson, E.R., Okazaki, R., Bonde, S., McDonagh, A.F., Riggs, B.L. J. Clin. Invest. (1995) [Pubmed]
  29. Schistosomula of Schistosoma mansoni use lysophosphatidylcholine to lyse adherent human red blood cells and immobilize red cell membrane components. Golan, D.E., Brown, C.S., Cianci, C.M., Furlong, S.T., Caulfield, J.P. J. Cell Biol. (1986) [Pubmed]
  30. Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment. Klopfenstein, D.R., Klumperman, J., Lustig, A., Kammerer, R.A., Oorschot, V., Hauri, H.P. J. Cell Biol. (2001) [Pubmed]
  31. Regulation of ubiquitin ligase dynamics by the nucleolus. Mekhail, K., Khacho, M., Carrigan, A., Hache, R.R., Gunaratnam, L., Lee, S. J. Cell Biol. (2005) [Pubmed]
  32. The double bromodomain protein Brd4 binds to acetylated chromatin during interphase and mitosis. Dey, A., Chitsaz, F., Abbasi, A., Misteli, T., Ozato, K. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  33. Nuclear dynamics of PCNA in DNA replication and repair. Essers, J., Theil, A.F., Baldeyron, C., van Cappellen, W.A., Houtsmuller, A.B., Kanaar, R., Vermeulen, W. Mol. Cell. Biol. (2005) [Pubmed]
  34. Repression of PML nuclear body-associated transcription by oxidative stress-activated Bach2. Tashiro, S., Muto, A., Tanimoto, K., Tsuchiya, H., Suzuki, H., Hoshino, H., Yoshida, M., Walter, J., Igarashi, K. Mol. Cell. Biol. (2004) [Pubmed]
  35. Distinct in vivo dynamics of vertebrate SUMO paralogues. Ayaydin, F., Dasso, M. Mol. Biol. Cell (2004) [Pubmed]
  36. Reversible loss of crystallinity on photobleaching purple membrane in the presence of hydroxylamine. Möller, C., Büldt, G., Dencher, N.A., Engel, A., Müller, D.J. J. Mol. Biol. (2000) [Pubmed]
  37. Characterization of a range of fura dyes with two-photon excitation. Wokosin, D.L., Loughrey, C.M., Smith, G.L. Biophys. J. (2004) [Pubmed]
  38. Lateral diffusion and phase separation in two-dimensional solutions of polymerized butadiene lipid in dimyristoylphosphatidylcholine bilayers. A photobleaching and freeze fracture study. Gaub, H., Sackmann, E., Büschl, R., Ringsdorf, H. Biophys. J. (1984) [Pubmed]
  39. Promotion of the release of 11-cis-retinal from cultured retinal pigment epithelium by interphotoreceptor retinoid-binding protein. Carlson, A., Bok, D. Biochemistry (1992) [Pubmed]
  40. Heterogeneous photobleaching in confocal microscopy caused by differences in refractive index and excitation mode. Van Oostveldt, P., Verhaegen, F., Messens, K. Cytometry. (1998) [Pubmed]
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