Disease relevance of Cacybp

• Using a pGEX vector the recombinant CacyBP was expressed in Escherichia coli, and its properties were analyzed [1].
• A protein target of mouse calcyclin, p30, which we call calcyclin-binding protein (CacyBP), was identified in mouse brain and Ehrlich ascites tumor (EAT) cells [1].
• Such perinuclear localization of CacyBP was observed in untreated neuroblastoma NB-2a cells in which [Ca(2+)](i) is approximately 120 nm [2].
• The expression of CacyBP in gastric cancer cells was determined by Northern and Western blots [3].

High impact information on Cacybp

• In untreated neuroblastoma NB-2a cells, CacyBP is phosphorylated on a serine residue(s), but exists in the dephosphorylated form in BAPTA/AM-treated cells [2].
• Thus, phosphorylation of CacyBP occurs in the same [Ca(2+)](i) range that leads to its perinuclear translocation [2].
• We have demonstrated recently that the Inv/Spa-secreted SopB and SopD effector proteins are translocated into eukaryotic cells via a Sip-dependent pathway and act in concert to mediate inflammation and fluid secretion in infected ileal mucosa [4].
• Because CacyBP, like calcyclin, is present in the brain, the interaction of these two proteins might be involved in calcium signaling pathways in neuronal tissue [1].
• The nucleotide and predicted amino acid sequences indicate that CacyBP is a novel protein [1].

Biological context of Cacybp

• CacyBP may be involved in the regulation of endometrial cell apoptosis during early pregnancy and play an important role in mouse endometrial events such as pregrancy establishment [5].
• In a GenBank search of the five putatively regulated cDNA fragments, two fragments shared high sequence homology to two known genes: the Surf-6 gene and the gene for calcyclin binding protein [6].

Anatomical context of Cacybp

• Most notably, CacyBP accumulation in trophoblast was pronounced at day 7 [5].
• Antisense oligonucleotides (ODNs) against CacyBP significantly inhibited cultured endometrial stromal cells' (ESCs) apoptosis induced by UV irradiation [5].
• Expression and hormonal regulation of calcyclin-binding protein (CacyBP) in the mouse uterus during early pregnancy [5].
• Western blot and immunofluorescence microscopy showed that the MAb BD1 against CacyBP could recognize CacyBP protein derived from human gastric cancer cell lines in both native and denatured forms [7].

Associations of Cacybp with chemical compounds

• While prominently expressed in both luminal and glandular epithelia, CacyBP underwent dynamic changes during early pregnancy [5].
• Using ovariectomized and pseudopregnant mice, we found that progesterone (P(4)) and 17beta-estradiol (E(2)) led to increased expression of CacyBP gene and this could be abolished by Ru486 and tamoxifen, respectively [5].
• SGC7901-CacyBP and SGC7901/ADR-anCacyBP cells displayed no obvious difference ( P > 0.05) in intracellular adriamycin content compared to their corresponding parental cells [3].
• BALB/c mouse splenocytes were immunized with purified 6 x His-CacyBP fusion protein and fused with myeloma cells using polyethylene glycol (PEG) 4000 [7].

Other interactions of Cacybp

• Calcyclin-binding protein (Siah-1-Interacting Protein, CacyBP/SIP), is a calcium signaling protein involved in the degradation of beta-catenin, however, little is known about its role in reproductive biology [5].

Analytical, diagnostic and therapeutic context of Cacybp

• The results obtained from northern blots show that the CacyBP gene is expressed predominantly in mouse brain and EAT cells [1].
• Ca(2+)-induced translocation of CacyBP was confirmed by immunogold electron microscopy and by fluorescence of NB-2a cells transfected with an EGFP-CacyBP vector [2].
• Using CacyBP/SIP affinity chromatography, we found that S100B from the brain extract binds to CacyBP/SIP in a Ca2+-dependent manner [8].
• Using ELISA, most (99%) women and newborns (97%) had serum Sip antibodies, as did most newborns followed through 6 months [9].
• CacyBP could regulate the responses of gastric cancer cells to chemotherapy [3].

References