The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Ncf2  -  neutrophil cytosolic factor 2

Mus musculus

Synonyms: 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, NADPH oxidase subunit (67kDa), NCF-2, NOXA2, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Ncf2

  • Sequencing, expression, and functional analyses support the candidacy of Ncf2 in susceptibility to Salmonella typhimurium infection in wild-derived mice [1].

High impact information on Ncf2


Biological context of Ncf2

  • Both p40phox and p67phox comprise several protein-binding modules that are structurally and functionally well conserved between mouse and human, indicating their nature as adaptor proteins [6].
  • We have investigated the relationship between the expression of the p47-phox and p67-phox cytosolic components of the NADPH oxidase and priming of the macrophage respiratory burst [7].
  • The level of the p22/p47/p67-phox subunit mRNA, and of apoptosis in tubular epithelial cells, was also increased compared with those from wild-type kidneys [8].
  • RESULTS: Obstructed kidneys from acatalasemic mice exhibited increased tubulointerstitial deposition in dilated tubules of collagens type I and IV, lipid peroxidation products, and the p22/p47/p67-phox subunits of NADPH oxidase [8].
  • The upregulation by peptide 2 was confirmed by the activation of the O2- -generating pathway, i.e., activation of large-molecule guanine binding protein, phosphatidyl-inositol 3-kinase, protein kinase C, and p47phox as well as p67phox [9].

Anatomical context of Ncf2

  • Because of the important role neutrophils play in bovine host defense, we carried out studies to clone, sequence, and express bovine p47-phox and p67-phox [10].
  • Here we describe how we cloned mouse cDNAs encoding p40phox and its partner in phagocytes, p67phox [6].
  • Expression of gp91phox and p67phox was increased in reactive astrocytes, which showed increased rates of both resting and stimulated ROS generation [11].

Associations of Ncf2 with chemical compounds


Other interactions of Ncf2

  • Membrane translocation of p47phox and p67phox as well as Rac1 activation was not increased further by combined PMA and AA stimulation [14].
  • Although not required for translocation of p47phox and p67phox, Rac2 is necessary for optimal activity of the assembled oxidase complex, an effect that can be replaced by exogenous AA, which may act directly or via an exogenous AA-induced mediator [14].
  • Here we describe two proteins, p41 and p51, with significant homology to two cytosolic components of the phagocytic oxidase, p47phox and p67phox [4].

Analytical, diagnostic and therapeutic context of Ncf2

  • The level of apoptosis was determined by terminal deoxynucleotidyl transferase nick end-labelling analysis, while the mRNA level of the p22-, p47- and p67-phox subunits was quantified by real-time PCR [8].
  • Site-directed mutagenesis and knockout experiments have identified the critical phosphorylation sites in p47-phox, revealed an "activation domain" in p67-phox, and demonstrated that a specific pathway exists for activating Rac to participate in oxidase assembly/activation [15].


  1. Sequencing, expression, and functional analyses support the candidacy of Ncf2 in susceptibility to Salmonella typhimurium infection in wild-derived mice. Sancho-Shimizu, V., Malo, D. J. Immunol. (2006) [Pubmed]
  2. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Francke, U., Hsieh, C.L., Foellmer, B.E., Lomax, K.J., Malech, H.L., Leto, T.L. Am. J. Hum. Genet. (1990) [Pubmed]
  3. Rac2 regulates neutrophil chemotaxis, superoxide production, and myeloid colony formation through multiple distinct effector pathways. Carstanjen, D., Yamauchi, A., Koornneef, A., Zang, H., Filippi, M.D., Harris, C., Towe, J., Atkinson, S., Zheng, Y., Dinauer, M.C., Williams, D.A. J. Immunol. (2005) [Pubmed]
  4. Proteins homologous to p47phox and p67phox support superoxide production by NAD(P)H oxidase 1 in colon epithelial cells. Geiszt, M., Lekstrom, K., Witta, J., Leto, T.L. J. Biol. Chem. (2003) [Pubmed]
  5. Synaptic localization of a functional NADPH oxidase in the mouse hippocampus. Tejada-Simon, M.V., Serrano, F., Villasana, L.E., Kanterewicz, B.I., Wu, G.Y., Quinn, M.T., Klann, E. Mol. Cell. Neurosci. (2005) [Pubmed]
  6. Functional modules and expression of mouse p40(phox) and p67(phox), SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex. Mizuki, K., Kadomatsu, K., Hata, K., Ito, T., Fan, Q.W., Kage, Y., Fukumaki, Y., Sakaki, Y., Takeshige, K., Sumimoto, H. Eur. J. Biochem. (1998) [Pubmed]
  7. Expression of p47-phox and p67-phox proteins in murine bone marrow-derived macrophages: enhancement by lipopolysaccharide and tumor necrosis factor alpha but not colony stimulating factor 1. Green, S.P., Hamilton, J.A., Uhlinger, D.J., Phillips, W.A. J. Leukoc. Biol. (1994) [Pubmed]
  8. Telmisartan inhibits both oxidative stress and renal fibrosis after unilateral ureteral obstruction in acatalasemic mice. Sugiyama, H., Kobayashi, M., Wang, D.H., Sunami, R., Maeshima, Y., Yamasaki, Y., Masuoka, N., Kira, S., Makino, H. Nephrol. Dial. Transplant. (2005) [Pubmed]
  9. Regulation of fungal infection by a combination of amphotericin B and peptide 2, a lactoferrin peptide that activates neutrophils. Okamoto, T., Tanida, T., Wei, B., Ueta, E., Yamamoto, T., Osaki, T. Clin. Diagn. Lab. Immunol. (2004) [Pubmed]
  10. Cloning and expression of bovine p47-phox and p67-phox: comparison with the human and murine homologs. Bunger, P.L., Swain, S.D., Clements, M.K., Siemsen, D.W., Davis, A.R., Gauss, K.A., Quinn, M.T. J. Leukoc. Biol. (2000) [Pubmed]
  11. Expression and modulation of an NADPH oxidase in mammalian astrocytes. Abramov, A.Y., Jacobson, J., Wientjes, F., Hothersall, J., Canevari, L., Duchen, M.R. J. Neurosci. (2005) [Pubmed]
  12. NADPH oxidase accounts for enhanced superoxide production and impaired endothelium-dependent smooth muscle relaxation in BKbeta1-/- mice. Oelze, M., Warnholtz, A., Faulhaber, J., Wenzel, P., Kleschyov, A.L., Coldewey, M., Hink, U., Pongs, O., Fleming, I., Wassmann, S., Meinertz, T., Ehmke, H., Daiber, A., Münzel, T. Arterioscler. Thromb. Vasc. Biol. (2006) [Pubmed]
  13. Adrenomedullin, an endogenous peptide, counteracts cardiovascular damage. Shimosawa, T., Shibagaki, Y., Ishibashi, K., Kitamura, K., Kangawa, K., Kato, S., Ando, K., Fujita, T. Circulation (2002) [Pubmed]
  14. Impaired NADPH oxidase activity in Rac2-deficient murine neutrophils does not result from defective translocation of p47phox and p67phox and can be rescued by exogenous arachidonic acid. Kim, C., Dinauer, M.C. J. Leukoc. Biol. (2006) [Pubmed]
  15. Regulation of the NADPH-oxidase complex of phagocytic leukocytes. Recent insights from structural biology, molecular genetics, and microscopy. Robinson, J.M., Ohira, T., Badwey, J.A. Histochem. Cell Biol. (2004) [Pubmed]
WikiGenes - Universities