Disease relevance of DPYSL3

• Structure and promoter analysis of the human unc-33-like phosphoprotein gene. E-box required for maximal expression in neuroblastoma and myoblasts [1].
• HUlip, a human homologue of unc-33-like phosphoprotein of Caenorhabditis elegans; Immunohistochemical localization in the developing human brain and patterns of expression in nervous system tumors [2].
• Validated genes included dopa decarboxylase, dopamine beta-hydroxylase, and dihydropyrimidinase-related protein 3, which were decreased in NBFL cells exposed to valproate, and spinocerebellar ataxia 7, which was increased in bipolar disease [3].
• The 3 oldest children who stutter showed evidence of tremorlike oscillations of EMG activity in the 5 to 15 Hz range during stuttering in either ULIP, LLIP, or ABD muscles [4].

High impact information on DPYSL3

• Giant cells in tubers, SEGAs and subependymal nodules in the Eker rat model of TSC expressed CRMP4 and DCX [5].
• However, the growth cone collapse-inducing protein Sema3A increases Cdk5 activity and promotes phosphorylation of CRMP2 (but not CRMP4) [6].
• Moreover, chondroitin sulfate and CRMP-4 were found to be localized immuno-histochemically in overlapping distributions in the marginal zone and the subplate of the cerebral cortex [7].
• CRMPs are released to culture supernatants of NTera-2 precursor cells and of neocortical neurons after cell death, and CRMP-4 is strongly expressed in the upper cortical plate of neonatal rat where cell death is abundant [7].
• Differential expression of collapsin response mediator proteins (CRMP/ULIP) in subsets of oligodendrocytes in the postnatal rodent brain [8].

Biological context of DPYSL3

• The LocusLink distribution in chromosomes obtained from both specimens was not significantly different; 17 of 19 putative genes (both specimen A and specimen B) were observed in the UniGene cluster in Homo sapiens, except for dihydropyrimidinase-related protein-3 and diacyglycerol kinase alpha [9].

Anatomical context of DPYSL3

• We have found pronounced CRMP-4 immunolabeling in certain neurons of the medial cortex, the homologous region to the dentate gyrus, but also in the dorsal and lateral cortices [10].
• Our results show for the first time the expression of CRMP-4 in a reptile brain, where it appears to be expressed in regions where adult neurogenesis and/or neurite outgrowth occur [10].

Associations of DPYSL3 with chemical compounds

• A specific protein-DNA complex, termed DRP-3, was identified in an electrophoretic mobility shift assay (EMSA) using a cisplatin-damaged DNA probe [11].

Other interactions of DPYSL3

• Autoantibodies produced against member(s) of this family by patients with paraneoplastic neurological diseases have made it possible to clone a fifth human Ulip/CRMP and characterize its cellular and anatomical distribution in developing brain [12].

Analytical, diagnostic and therapeutic context of DPYSL3

• Transcripts encoding the other CRMP/Ulip members are also detectable by RT-PCR in highly purified mature oligodendrocytes [8].
• Since CRMP-4 protein appears to be highly conserved throughout the evolutionary scale, we have used immunocytochemistry to study its distribution in the lizard cerebral cortex [10].

References