Psychiatry related information on Sf1

• We describe here a novel reporter mouse line that expresses enhanced green fluorescent protein under the control of an Sf1 promoter fragment, marking Sertoli and granulosa cell precursors during the critical period of sex determination [1].

High impact information on Sf1

• 5. However, already at E12.5 the 'adrenal' region in Sf1-/- mice is occupied by fewer PHOX2B+ and TH+ SA cells as well as SOX10+ neural crest cells [2].
• We show that in Sf1-/- mice typical chromaffin cells assemble correctly in the suprarenal region adjacent to the suprarenal sympathetic ganglion [2].
• Nuclear receptors Sf1 and Dax1 function cooperatively to mediate somatic cell differentiation during testis development [3].
• Lack of an adrenal cortex in Sf1 mutant mice is compatible with the generation and differentiation of chromaffin cells [2].
• Similar abnormalities appear to be present in humans.Targeted mutagenesis of Sf1 (FtzF1) prevents gonadal and adrenal development, and causes male-to-female sex-reversal [4].

Biological context of Sf1

• Presumably, MZFM is a new member of those proteins combining features of signal transduction and RNA activation (STAR-proteins) [5].
• We have characterized the cDNA of MZFM, the mouse homolog to the novel human putative tumor suppressor gene ZFM1 [5].
• In two of the three mutant clones, this Sf1 allele is amplified together with flanking DNA from chromosome 2 that includes the genes encoding germ cell nuclear factor and the beta-type proteosome subunit Psmb7 [6].
• L2949 has significant similarity to the human ZFM1 (related to a potential suppressor oncogene) and mouse CW17R genes, though it lacks the carboxy-terminal oligoproline and oligoglutamine stretches encoded by these mammalian genes [7].
• An examination of the Sf-1 transcript stability in the presence of actinomycin D revealed no influence of TGF-beta on the rate of Sf-1 mRNA decay [8].

Anatomical context of Sf1

• Northern analysis reveals expression of MZFM only in spleen macrophages [5].
• The MZFM cDNA best matches to that ZFM1-isoform without the so-called 0.25-kb E-domain and to the L49345 cDNA recently identified in a human leukemia cell line [5].

Associations of Sf1 with chemical compounds

• Inhibition of Sf-1 expression by TGF-beta was abolished by cycloheximide, suggesting that the growth factor inhibitory effect requires ongoing protein synthesis [8].

Other interactions of Sf1

• Much has been learned about the role of this transcription factor since its initial cloning in 1992, largely due to the creation of an Sf-1 (FtzF1) knockout mouse model, in vitro studies of nuclear receptor function and, more recently, following identification and characterization of patients with naturally-occurring SF-1 mutations [9].
• In this report, we show that ACTH resistance in the mutant clones is associated with a Sf1 gene that has Ser at codon 172 instead of Ala [6].

References