High impact information on MAPK8IP3

• Leukocyte common antigen-related (LAR) family receptor protein tyrosine phosphatases (LAR-RPTP) bind to liprin-alpha (SYD2) and are implicated in axon guidance [1].
• Pin1 is enriched at the mitochondrial membrane in neurons, where it forms a physical complex with the neuron-specific JNK scaffold protein JIP3 [2].
• The activated SEK1 dissociates from JIP3 and phosphorylates JNK2 on the Tyr-185 residue [3].
• In the present study, we reveal that two scaffolding proteins, JIP1 and JIP3, have a cross-talk that leads to the regulation of the ASK1-SEK1-JNK signal during glucose deprivation [3].
• Liprin-alpha/SYD-2 is a family of multidomain proteins with four known isoforms [4].

Biological context of MAPK8IP3

• In the present study, we investigated the function of JSAP1 during PC12h cell differentiation by knocking down the level of JSAP1 [5].
• Furthermore, we found by Western blotting that the expression levels of JSAP1 protein vary during development; there is high expression until 6 weeks after birth, which approximately corresponds to the end of the first wave of spermatogenesis [6].

Anatomical context of MAPK8IP3

• Expression of JSAP1 in Hela cells facilitated formation of well-organized focal contacts and actin stress fibers, and promoted cell spreading onto FN [7].
• In addition, two known JNK3-interacting proteins, beta-arrestin 2 and JIP3, play established roles in neurite outgrowth and neurological development [8].
• Although JSAP1 is expressed in the testis in adults, its expression during development has not been investigated [6].
• Selective expression of the scaffold protein JSAP1 in spermatogonia and spermatocytes [6].
• The immunohistochemical analyses with the specific antibody showed that the JSAP1 protein was selectively expressed in the spermatogonia and spermatocytes, but not in other cell types, including spermatids and somatic cells, during development [6].

Associations of MAPK8IP3 with chemical compounds

• Taken together, these results suggest that JSAP1 is involved an integrin-mediated signaling pathway through FAK/Src by recruiting other signaling molecules, resulting in promotion of cell spreading onto FN [7].

Physical interactions of MAPK8IP3

• JSAP1 (also termed JIP3) is a scaffold protein that interacts with specific components of the JNK signaling pathway [9].

Enzymatic interactions of MAPK8IP3

• The complex formation was further stimulated by c-Src, in which JSAP1 was tyrosine-phosphorylated and other FAK/Src signaling molecules were recruited [7].

Other interactions of MAPK8IP3

• We previously reported that c-Jun NH(2)-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1) functions as a putative scaffold factor in the JNK mitogen-activated protein kinase (MAPK) cascades [10].
• Here we have defined the regions of JSAP1 responsible for the interactions with MEK1 and Raf-1 [10].
• Taken together, these results suggest that JSAP1 regulates cell-cell interactions in PC12h cells specifically in the NGF-induced signaling pathway, and does so by modulating N-cadherin [5].

References