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Gene Review

SAMHD1  -  SAM domain and HD domain 1

Homo sapiens

Synonyms: AGS5, CHBL2, DCIP, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, ...
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Disease relevance of SAMHD1


High impact information on SAMHD1

  • In the presence of 75 microM CB 1954, a 50% cell killing was achieved in cells containing Q104Y and the wild-type DT-diaphorase with the activity at approximately 0.67 and 3.8 micromol of DCIP reduced/min/mg of protein, respectively [4].
  • The concentration of photosystem II was determined in thylakoids of dwarf peas by the use of the following methods: absorption change at 325 nm; atrazine binding; and flash yields of oxygen evolution (Emerson-Arnold method), of protons from oxidation of water, and of reduction of DCIP [5].
  • Superoxide dismutase (SOD) and aminooxyacetate (AOA), a malate/aspartate shuttle inhibitor, strongly inhibited WST-1 reduction and reduced DCIP reduction by 40-60%, but failed to affect FeCN reduction, indicating involvement of mitochondrial TCA cycle-derived NADH and a possible role for superoxide in WST-1 but not FeCN reduction [6].
  • The site of inhibition of isoalloalantolactone is located in the span of P680 to QA redox enzymes because the uncoupled electron transport from water to silicomolybdate and, from DPC to DCIP are inhibited approximately to the same extent [7].
  • Plasma membrane NADH oxidoreductase activity, studied using cytosolic NADH as substrate and assayed with DCIP as acceptor, was significantly increased in IDDM patients, suggesting a response to a deficient mitochondrial energetic activity [8].

Anatomical context of SAMHD1

  • Plasma membrane oxidoreductases have been described in all cells and use extracellular impermeant electron acceptors (DCIP, Ferricyanide) that are reduced by NADH [9].

Associations of SAMHD1 with chemical compounds

  • Further it was observed that reduced DCIP could be oxidized by DBMIB in the absence of NADH indicating that the electron flow in the nitrate reductase complex may take place in a unidirectional way [10].
  • The DCIP activity was related to cell protein (r = 0.39), protein concentration in decidual culture medium (r = 0.36), and inversely related to the glucose level (r = -0.41) [3].
  • The chloroplasts from wheat leaves greened under intermittent illuminations (1 ms in duration) at long intervals (5 min) are capable of photoreducing DCIP (2,6-dichlorophenolindophenol) with diphenylcarbazide as an electron donor but are incapable of photoreducing DCIP with water as the donor [11].
  • Both NADPH- and DCIP-requiring enzyme activities which could not be separated by our enzyme purification system were found in the same single protein band on polyacrylamide disc gel electrophoresis [12].

Analytical, diagnostic and therapeutic context of SAMHD1

  • At the end of each experiment the DCIP in the decidual culture medium was measured by bioassay: the percentage reduction, from control, of hCG production by JEG-3 cells exposed to 30% DCIP-containing decidual culture medium [3].


  1. Delineation of the catalytic components of the NADPH-dependent O2- generating oxidoreductase of human neutrophils. Green, T.R., Wirtz, M.K., Wu, D.E. Biochem. Biophys. Res. Commun. (1983) [Pubmed]
  2. Screening for thalassemia and hemoglobinopathy in a rural area of Thailand: a preliminary study. Nathalang, O., Nillakupt, K., Arnutti, P., Boonsiri, T., Panichkul, S., Areekul, W. Journal of the Medical Association of Thailand = Chotmaihet thangphaet. (2005) [Pubmed]
  3. Comparison of production of choriogonadotropin inhibitory protein, prolactin and insulin-like growth factor binding protein-1 by human decidua in vitro. Ren, S.G., Braunstein, G.D. Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy. (1996) [Pubmed]
  4. Demonstration of the activation of prodrug CB 1954 using human DT-diaphorase mutant Q104Y-transfected MDA-MB-231 cells and mouse xenograft model. Wu, K., Eng, E., Knox, R., Chen, S. Arch. Biochem. Biophys. (2001) [Pubmed]
  5. Reconciliation of the absorption change at 325 nm and other flash-yield determinations of concentrations of active photosystem II centers. Jursinic, P., Dennenberg, R. Arch. Biochem. Biophys. (1985) [Pubmed]
  6. Distinct trans-plasma membrane redox pathways reduce cell-impermeable dyes in HeLa cells. Tan, A.S., Berridge, M.V. Redox Rep. (2004) [Pubmed]
  7. Biochemically active sesquiterpene lactones from Ratibida mexicana. Calera, M.R., Soto, F., Sanchez, P., Bye, R., Hernandez-Bautista, B., Anaya, A.L., Lotina-Hennsen, B., Mata, R. Phytochemistry (1995) [Pubmed]
  8. Lymphocyte dysmetabolism: an immunocytochemical comparative approach in IDDM and control subjects. Pugnaloni, A., Sgarbi, G., Tesei, M., D'Aurelio, M., Ragni, L., Parenti Castelli, G., Salardi, S., Zucchini, S., Bovina, C., Cacciari, E., Lenaz, G., Biagini, G. European journal of histochemistry : EJH. (2001) [Pubmed]
  9. The plasma membrane NADH-diaphorase is active during selective phases of the cell cycle in mouse neuroblastoma cell line NB41A3. Its relation to cell growth and differentiation. Zurbriggen, R., Dreyer, J.L. Biochim. Biophys. Acta (1996) [Pubmed]
  10. Inhibition of the nitrate reductase complex by dibromothymoquinone. Jawali, N., Ramakrishna, J., Sainis, J.K., Sane, P.V. Z. Naturforsch., C, Biosci. (1979) [Pubmed]
  11. Multiple-flash activation of the water-photolysis system in wheat leaves as observed by delayed emission. Inoue, Y. Biochim. Biophys. Acta (1975) [Pubmed]
  12. NADPH-dependence of vitamin B2-aldehyde-forming enzyme. Tachibana, S., Oka, M. J. Nutr. Sci. Vitaminol. (1982) [Pubmed]
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