Disease relevance of BLVRB

• Flavin reductase catalyzes electron transfer from reduced pyridine nucleotides to methylene blue or riboflavin, and this catalysis is the basis of the therapeutic use of methylene blue or riboflavin in the treatment of methemoglobinemia [1].
• These cells released GHBP of the expected size, and this release could be increased by incubation with a phorbol ester, which stimulated receptor synthesis through the cytomegalovirus promoter [2].
• Although these agents are known to be effective in the parent hepatoma line, none were effective in modulating GHBP release, although GH itself decreased release by around 30% as assessed with a ligand immunofunctional assay [2].

High impact information on BLVRB

• Degenerate oligonucleotides, with sequences based on amino acid sequences of peptides derived from bovine erythrocyte flavin reductase, were used as primers in PCR to selectively amplify a partial cDNA that encodes the bovine reductase [1].
• Flavin reductase: sequence of cDNA from bovine liver and tissue distribution [1].
• The flavin reductase system catalyzes the interconversion in both directions with dioxygen as the critical factor deciding whether activation or inactivation of ribonucleotide reductase occurs [3].
• Radical introduction into metB2 requires a flavin reductase together with a second poorly defined protein fraction ("Fraction b") as well as dioxygen, NAD(P)H, and a flavin (Fontecave, M., Eliasson, R., and Reichard, P. (1987) J. Biol. Chem. 262, 12325-12331) [3].
• In humans and rabbits, the circulating GH binding protein (GHBP) is released from the GH receptor by cleavage at a site proximal to the cell surface [2].

Biological context of BLVRB

• Specific binding of 125I-labelled human GH (hGH) to plasma GHBP was easily detected as early as 75 days of gestation and increased with age (P < 0.001) [4].

Anatomical context of BLVRB

• Papain proteolysis releases a soluble NADPH dependent diaphorase activity from bovine neutrophil membranes [5].

Associations of BLVRB with chemical compounds

• Plasma GHBP rose in female rats given E2, and fell in females given the anti-estrogen tamoxifen [6].
• We examined the ability of IGF-1, insulin, dexamethasone, sex steroids, and T4 to influence GHBP release [2].
• In muscle, octreotide was associated with an approximately 30% decrease in GHBP mRNA and no effect on GHR mRNA [7].
• Activities of succinate oxidase, fumarate reductase (FR) and succinate dehydrogenase (SDH) under a set of defined conditions were determined in the mitochondrial isolate from Setaria digitata, the filarial parasite from the cattle Bos indicus [8].
• Though SDH and FR catalyse reverse reactions, they responded differently to modulators such as oxaloacetate, aspartate, alanine, pyruvate and fumarate [8].

Analytical, diagnostic and therapeutic context of BLVRB

• Serum growth hormone-binding protein (GHBP) in domestic animals as measured by ELISA [9].
• The GHBP was partially purified from chicken, ovine, and porcine serum using GH affinity chromatography [10].
• GH-GHBP complex formation was performed by high-performance liquid chromatography, and the radioactivity was recorded on-line with a Berthold LB detector connected to a computer [11].
• FTIR difference spectroscopy is used to reveal changes in the internal structure and amino acid protonation states of bovine cytochrome c oxidase (CcO) that occur upon photolysis of the CO adduct of the two-electron reduced (mixed valence, MV) and four-electron reduced (fully reduced, FR) forms of the enzyme [12].

References