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Gene Review:

Tb10.70.5120 - malate dehydrogenase

Trypanosoma brucei brucei TREU927

Leroux, A. et al., Opperdoes, F.R. et al., Markos, A. et al., Voorheis, H.P. et al., van Weelden, S.W. et al., et al.

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Disease relevance of Tb10.70.5120

NADP-linked malic enzyme (malate dehydrogenase (oxaloacetate-decarboxylating) NADP+, EC 1.1.1.40) has been partially purified from adult Onchocerca volvulus and Dirofilaria immitis [1].

High impact information on Tb10.70.5120

We suggest that citrate synthase plus pyruvate dehydrogenase and malate dehydrogenase are used to transport acetyl-CoA units from the mitochondrion to the cytosol for the biosynthesis of fatty acids, a process we show to occur in proliferating procyclic cells [2].
The purified membranes were essentially free of L-alanine-alpha-oxoglutarate aminotransferase, L-asparte-alpha-oxoglutarate aminotransferase, malate dehydrogenase, oligomycin-sensitive adenosine triphosphatase, glucose 6-phosphatase, Mg2+-stimulated p-nitrophenyl phosphatase and catalase [3].
Localization of malate dehydrogenase, adenylate kinase and glycolytic enzymes in glycosomes and the threonine pathway in the mitochondrion of cultured procyclic trypomastigotes of Trypanosoma brucei [4].
This increase in activity was the result of the appearance of malate dehydrogenase in the glycosomes of the procyclics, in addition to mitochondrial and cell-sap activities which were present in both stages of the life cycle [4].
Glycosomal phosphoenolpyruvate carboxykinase and malate dehydrogenase were identified to have subunit molecular weights of 60 and 34 kDa, respectively [5].

Associations of Tb10.70.5120 with chemical compounds

Activities of procyclic-stage enzyme markers malate dehydrogenase, threonine dehydrogenase, succinate: cytochrome c reductase, and NADH: cytochrome c reductase rose within 48 h of differentiation to values which were close to those found in established procyclic forms [6].

Other interactions of Tb10.70.5120

Consistent differences between bloodstream and culture forms were observed for alanine aminotransferase, aspartate aminotransferase and malate dehydrogenase [7].

Analytical, diagnostic and therapeutic context of Tb10.70.5120

Western blot analysis showed that the three MDHs are developmentally regulated [8].
To further characterize the leishmanial isozymes, three putative MDH genes, presumably corresponding to the mitochondrial, glycosomal and cytosolic isoforms were amplified by PCR, cloned into bacterial expression vectors, and the recombinant enzymes purified [8].
Gel filtration chromatography in native conditions showed that most of the MDH activity present in the crude extracts eluted in a single peak, which corresponded to a lower apparent molecular mass ( congruent with 57kDa) than the value expected for typical MDHs [8].
Trypanosoma brucei: molecular cloning and stage-regulated expression of a malate dehydrogenase localized to the mitochondrion [9].

References

Inhibition of NADP-linked malic enzyme from Onchocerca volvulus and Dirofilaria immitis by suramin. Walter, R.D., Albiez, E.J. Mol. Biochem. Parasitol. (1981) [Pubmed]
New functions for parts of the Krebs cycle in procyclic Trypanosoma brucei, a cycle not operating as a cycle. van Weelden, S.W., van Hellemond, J.J., Opperdoes, F.R., Tielens, A.G. J. Biol. Chem. (2005) [Pubmed]
The isolation and partial characterization of the plasma membrane from Trypanosoma brucei. Voorheis, H.P., Gale, J.S., Owen, M.J., Edwards, W. Biochem. J. (1979) [Pubmed]
Localization of malate dehydrogenase, adenylate kinase and glycolytic enzymes in glycosomes and the threonine pathway in the mitochondrion of cultured procyclic trypomastigotes of Trypanosoma brucei. Opperdoes, F.R., Markoŝ, A., Steiger, R.F. Mol. Biochem. Parasitol. (1981) [Pubmed]
Trypanosoma brucei: two-dimensional gel analysis of the major glycosomal proteins during the life cycle. Parsons, M., Nielsen, B. Exp. Parasitol. (1990) [Pubmed]
Metabolic differentiation of bloodstream forms of Trypanosoma brucei brucei into procyclic forms. Effect of hydroxyurea, arabinosyl adenine, and serum omission. Markos, A., Blahůsková, A., Kalous, M., Bysková, E., Byska, K., Nohýnková, E. Folia Parasitol. (1989) [Pubmed]
The substitution of procyclic for bloodstream form Trypanosoma brucei gambiense in isoenzyme studies. Kaukas, A., Gashumba, J.K., Lanham, S.M., Dukes, P. Trans. R. Soc. Trop. Med. Hyg. (1990) [Pubmed]
Functional characterization and subcellular localization of the three malate dehydrogenase isozymes in Leishmania spp. Leroux, A., Fleming-Canepa, X., Aranda, A., Maugeri, D., Cazzulo, J.J., Sánchez, M.A., Nowicki, C. Mol. Biochem. Parasitol. (2006) [Pubmed]
Trypanosoma brucei: molecular cloning and stage-regulated expression of a malate dehydrogenase localized to the mitochondrion. Anderson, S.A., Carter, V., Parsons, M. Exp. Parasitol. (1998) [Pubmed]

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