High impact information on LIPE

• The enzyme was distinguishable in physical and kinetic properties from the two previously defined lipases in adipose tissue, lipoprotein lipase, and hormone-sensitive lipase [1].
• In these cells, the age-related increase (+300%) in the ratio of mRNA levels of fatty acid synthase to hormone-sensitive lipase paralleled the enlargement of adipocyte diameters (+70%, P < 0.05) and the increase in lipid content in muscle (+135%, P < 0.05) during growth [2].
• In addition, HSL was studied in subcellular fractions obtained from STf isolated from adult testes and in epididymal spermatozoa (Spz) [3].
• The HSL activity was positively correlated with free (FC) and esterified (EC) cholesterol ratios in STf and ITf, but not with triglyceride (TG) levels, during testicular development [3].
• The findings of the 104- and 120-kDa HSL immunoreactive bands and of HSL activity in Spz as well, as the detection of the 104-, 110-, and 120-kDa immunoreactive bands in lysosomes, suggest that part of HSL may originate from germ cells and be imported in Sertoli cells [3].

Biological context of LIPE

• GRP78 was mapped to 1q2.10-->q2.13 and LIPE was localized to chromosome 6cen-->q1 [4].
• These experiments demonstrated that the gene order was cen-GPI-CRC-LIPE [5].
• FISH on metaphase and interphase chromosomes demonstrates the physical order of the genes for GPI, CRC, and LIPE in pigs [5].
• Hormone-sensitive lipase activity on d 28 of lactation was increased by the addition of tallow to the diet (P = .06) [6].
• The HSL is activated by phosphorylation [7].

Anatomical context of LIPE

• Differential responses of hormone-sensitive lipase gene to nutritional transition in adipose tissue, liver, and skeletal muscle of pigs [8].
• With more sensitive PCR method, hormone-sensitive lipase mRNA was found in adipose tissue, liver, heart, skeletal muscle, testis, and spleen [8].

Associations of LIPE with chemical compounds

• The HSL protein levels and enzymatic activity in ITf and STf were positively correlated with serum testosterone levels during development [3].
• The effect of dietary ethanol on metabolic fates of glucose and ethanol, and activities of lipoprotein lipase and hormone-sensitive lipase in several tissues of miniature pigs were determined in vitro [9].
• Addition of fat to the diets of lactating sows: II. Effects on hormone-sensitive lipase activity, energy mobilization in response to epinephrine, and plasma insulin and glucose concentrations [6].
• However, there was no effect of diet on the activities of several of the enzymes controlling the lipolytic process, i.e., adenylate cyclase, phosphodiesterase and hormone-sensitive lipase [10].
• Hormone-sensitive lipase (HSL) is an adipocyte enzyme that cleaves fatty acids from intracellular triacylglycerol [7].

Other interactions of LIPE

• Chromosomal localization of the hormone sensitive lipase (LIPE) and insulin receptor (INSR) genes in pigs [11].
• The CCAAT/enhancer binding proteins beta and alpha, adipocyte fatty acid binding protein, hormone-sensitive lipase, and the beta1-adrenergic receptor transcript concentrations tended (P < . 10) to be greater in adipose tissue from obese than in that from lean pigs [12].

Analytical, diagnostic and therapeutic context of LIPE

• Linkage mapping located the LIPE gene close to the calcium release channel (CRC) locus on chromosome 6 [13].

References