Disease relevance of ROR1

• ROR1 mRNA was expressed in embryonic stem (ES) cells, infant brain, renal cancer, and colon cancer [1].
• We used a Ror1 cDNA probe to screen a cDNA library prepared from the human neuronogenic teratocarcinoma line, NTera2, and cloned a 2373 nucleotide transcript [2].
• Northern blots demonstrate that mRNA encoding this truncated Ror1 ('t-Rorl') is abundantly expressed in fetal and adult human CNS, in human leukemia, lymphoma cell lines, and in a variety of human cancers derived from neuroectoderm [2].
• WNT5A and ROR family receptors, co-expressed in ES cells and gastric cancer, are implicated in the planar cell polarity (PCP) pathway [1].

High impact information on ROR1

• Our work suggests important roles of ROR1/RPA2A in epigenetic gene silencing and in the regulation of plant development [3].
• Map-based cloning revealed that ROR1 encodes a 31-kD protein similar to DNA replication protein A2 (RPA2A) [3].
• Mutations in ROR1/RPA2A affect cell division in meristems but not final cell sizes [3].
• These results indicate that release of silenced Pro(35S):NPTII by ror1 mutations is independent of DNA methylation [3].
• MHR3, a homolog of the retinoid orphan receptor (ROR), is a transcription factor in the nuclear hormone receptor family that is induced by 20-hydroxyecdysone (20E) in the epidermis of the tobacco hornworm, Manduca sexta [4].

Biological context of ROR1

• Comparative genomics on ROR1 and ROR2 orthologs [1].
• Here, comparative integromics analyses on ROR1 and ROR2 orthologs were performed by using bioinformatics [1].
• ROR1 and ROR2 are the pharmacogenomics targets in the fields of stem cell biology and oncology [1].
• SH2 binding site within the RORHC domain was conserved among vertebrate ROR2 orthologs, but not among vertebrate ROR1 orthologs [1].
• Immunoglobulin-like domain, Frizzled domain, Kringle domain within the extracellular region, tyrosine kinase domain, Ror homology C-terminal (RORHC) domain and juxta-C-terminal LLGD motif within the cytoplasmic region were conserved among vertebrate ROR1 and ROR2 orthologs [1].

Anatomical context of ROR1

• Southern blot analysis of genomic DNA from somatic cell hybrids revealed that Ror1 is located on chromosome 1 [5].
• Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons [6].

Associations of ROR1 with chemical compounds

• We have cloned cDNA encoding a mouse nuclear receptor mROR alpha which is a homolog of human retinoic acid receptor-related orphan receptor (hROR alpha) [7].

Other interactions of ROR1

• ROR2 promoter rather than ROR1 promoter was more evolutionarily conserved [1].

Analytical, diagnostic and therapeutic context of ROR1

• We have further localized the gene to chromosome 1p31-p32 by fluorescence in situ hybridization using a PAC clone that contains the Ror1 gene [5].
• We have mapped the Ror1 gene to chromosome 1p12-p32 using PCR on a somatic cell hybrid panel that subdivides chromosome 1p [5].

References