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Gene Review

RPS27  -  ribosomal protein S27

Homo sapiens

Synonyms: 40S ribosomal protein S27, MPS-1, MPS1, Metallopan-stimulin 1, S27
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Disease relevance of RPS27


High impact information on RPS27

  • We propose that MPS1-dependent BLM phosphorylation is important for ensuring accurate chromosome segregation, and its deregulation may contribute to cancer [6].
  • MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability [6].
  • Using immunodepletion approach and a rephosphorylation assay in Xenopus extracts, we report here that not only the formation of the 3F3/2 phosphoepitope is dependent on the checkpoint activation but also the loading of the 3F3/2 substrate to kinetochores requires the prior assembly of Mps1, Bub1 and BubR1 onto kinetochores [7].
  • Metaphase arrest by cyclin e-cdk2 requires the spindle-checkpoint kinase mps1 [8].
  • Consistent with the recently identified vertebrate MPS1 homologues, we found that hMPS1 is localized to centrosomes and kinetochores [9].

Biological context of RPS27

  • We localized the kinetochore targeting domain in hMPS1 and found that it can abrogate the mitotic checkpoint in a dominant negative manner [9].
  • The carboxyl-terminal cysteine arrangement in hRPS27 is similar to the family of C4 zinc finger DNA-binding proteins [10].
  • A potential polyadenylation signal AACAAA is found in the MPS-1 cDNA but the more frequently used AATAAA sequence is present in the hRPS27 cDNA [10].
  • In contrast, the MPS-1 mRNA is expressed at low levels in normal WI-38 human lung diploid fibroblasts in culture [1].
  • Moreover, little is presently known about the regulation of Mps1 kinases during the cell cycle [11].

Anatomical context of RPS27

  • Characterization of the MPS-1 protein extracted from Sf9 infected cells showed that it: binds zinc ions specifically; is phosphorylated; accumulates in the nucleus; is tightly bound to the nucleus; and binds to calf thymus DNA-cellulose [2].
  • Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1 [11].
  • In contrast, no expression of MPS-1 was observed in the adjacent normal mucosa [5].

Associations of RPS27 with chemical compounds

  • These results support the hypothesis that the MPS-1 protein may act, at least in part, by interacting with genes possessing the cyclic AMP-responsive element sequence [2].
  • Furthermore, we have shown that detection of MPS-N immunoreactive material in sera corresponding to the NH2 terminus of MPS-1 provides a method for determining the presence of certain types of abnormal proliferative conditions and/or active oncogenic processes in patients [12].

Other interactions of RPS27


Analytical, diagnostic and therapeutic context of RPS27


  1. A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure. Fernandez-Pol, J.A., Klos, D.J., Hamilton, P.D. J. Biol. Chem. (1993) [Pubmed]
  2. Metallopanstimulin gene product produced in a baculovirus expression system is a nuclear phosphoprotein that binds to DNA. Fernandez-Pol, J.A., Klos, D.J., Hamilton, P.D. Cell Growth Differ. (1994) [Pubmed]
  3. Identification of metallopanstimulin-1 as a member of a tumor associated antigen in patients with breast cancer. Atsuta, Y., Aoki, N., Sato, K., Oikawa, K., Nochi, H., Miyokawa, N., Hirata, S., Kimura, S., Sasajima, T., Katagiri, M. Cancer Lett. (2002) [Pubmed]
  4. In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity. Wang, Y.W., Qu, Y., Li, J.F., Chen, X.H., Liu, B.Y., Gu, Q.L., Zhu, Z.G. Clin. Cancer Res. (2006) [Pubmed]
  5. Metallopanstimulin is overexpressed in a patient with colonic carcinoma. Ganger, D.R., Hamilton, P.D., Fletcher, J.W., Fernandez-Pol, J.A. Anticancer Res. (1997) [Pubmed]
  6. MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng, M., Chan, D.W., Luo, H., Zhu, C., Qin, J., Wang, Y. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  7. Loading of the 3F3/2 Antigen onto Kinetochores Is Dependent on the Ordered Assembly of the Spindle Checkpoint Proteins. Wong, O.K., Fang, G. Mol. Biol. Cell (2006) [Pubmed]
  8. Metaphase arrest by cyclin e-cdk2 requires the spindle-checkpoint kinase mps1. Grimison, B., Liu, J., Lewellyn, A.L., Maller, J.L. Curr. Biol. (2006) [Pubmed]
  9. Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores. Liu, S.T., Chan, G.K., Hittle, J.C., Fujii, G., Lees, E., Yen, T.J. Mol. Biol. Cell (2003) [Pubmed]
  10. Primary structures and sequence analysis of human ribosomal proteins L39 and S27. Tsui, S.K., Lee, S.M., Fung, K.P., Waye, M.M., Lee, C.Y. Biochem. Mol. Biol. Int. (1996) [Pubmed]
  11. Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. Stucke, V.M., Baumann, C., Nigg, E.A. Chromosoma (2004) [Pubmed]
  12. Expression of metallopanstimulin and oncogenesis in human prostatic carcinoma. Fernandez-Pol, J.A., Fletcher, J.W., Hamilton, P.D., Klos, D.J. Anticancer Res. (1997) [Pubmed]
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