Disease relevance of RRBP1

• FISH analyses of individuals with 20p12 deletions and affected by Alagille syndrome exclude hES as a candidate gene for this disorder [1].
• Early-stage mammalian embryos develop in a low O(2) environment (hypoxia). hES cells, however, are generally cultured under an atmosphere of 21% O(2) (normoxia), under which conditions they tend to differentiate spontaneously [2].

High impact information on RRBP1

• We propose that Mba1 functions as a ribosome receptor that cooperates with Oxa1 in the positioning of the ribosome exit site to the insertion machinery of the inner membrane [3].
• Mba1, a membrane-associated ribosome receptor in mitochondria [3].
• In addition, hES cell growth under hypoxia provided enhanced formation of embryoid bodies [2].
• The second hypothesis was that hypoxic culture would reduce the amount of spontaneous cell differentiation that occurs in hES colonies [2].
• Low O2 tensions and the prevention of differentiation of hES cells [2].

Biological context of RRBP1

• Fluorescence in situ hybridization analysis (FISH) localizes hES to human chromosome 20p11.2-p12 [1].
• The ES130/p180 gene was mapped to chromosome 20p12, and a potential pseudogene appears to reside on chromosome 7 [4].
• By a micro-amino-acid sequencing technique, the 180-kDa protein was identified as a human homologue of the ES130/p180 ribosome receptor (p180), which is an integral endoplasmic reticulum (ER) membrane protein possessing a very unique tandem repeat domain at its N-terminal region [5].
• Cytogenetic analysis of the hESCs revealed that hES-NCL1 line has a normal female (46, XX) karyotype [6].
• hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening [7].

Anatomical context of RRBP1

• Reverse transcriptase-polymerase chain reaction studies reveal that hES is expressed in both fetal and adult human tissues and that hES expression in the left ventricle is increased in the failing adult heart [1].
• We describe the generation of erythroid cells from hES (H1) by subsequent processing of cells present at early and late stages of embryoid body (EB) differentiation [8].
• The successful derivation of a human embryonic stem cell (hESC) line from blastocysts generated by somatic cell nuclear transfer (SCNT) provides proof-of-principle for "therapeutic cloning," though immune matching of the differentiated NT-hES remains to be established [9].
• These data provide the first detailed comparison of different hES cell lines and demonstrate remarkable similarities among lines maintained in identical culture conditions [10].
• This study was undertaken to determine whether discarded day 3 embryos with low morphological scores could develop into blastocysts and produce hES cell lines [11].

Associations of RRBP1 with chemical compounds

• One of the adsorbed proteins is a member of the leucine-rich repeat protein family termed ribosome-binding protein p34 (p34) [12].
• When transplanted into 6-hydroxydopamine-treated animals, hES-derived dopaminergic cells integrated into the rat striatum [13].
• Surprisingly, our results showed that immediately after post-thaw washing, the overwhelming majority of hES cells were viable (approximately 98%), as assessed by the trypan blue exclusion test [14].
• Ultrastructural and immunohistochemical studies of 4 groups of cells-(human embryonic stem cells (hES), embryoid bodies (EB), and spontaneously and retinoic acid (RA)-induced differentiating cells)-were carried out to investigate their detailed phenotype [15].

Physical interactions of RRBP1

• Identification of ribosome-binding protein p34 as an intracellular protein that binds acidic fibroblast growth factor [12].

Analytical, diagnostic and therapeutic context of RRBP1

• For such utilization of hES cells to be realized, however, protocols involved in the use of hES cells, such as those for establishment, propagation, and cryopreservation, have still to be improved [16].
• BACKGROUND: Immune rejection can lead to the failure of human embryonic stem cell (hES cell) transplantation [11].
• These results provide valuable information that will assist in achieving the goal of the large-scale hES cell culture required for the application of hES cells to disease therapy [16].
• Human ES (hES) cell lines are considered to be a valuable resource for medical research and for applications in cell therapy and drug discovery [16].
• Transmission electron microscopy showed that hES and EB cells were very similar to germ cells or cells of the inner cell mass [15].

References