Disease relevance of Dio2

• Increase in type II 5'deiodinase activity and the number of binding sites point to an adaptive increase in response to thiocyanate induced hypothyroidism to maintain the cellular T3 levels within a narrow limit [1].

High impact information on Dio2

• These data show that myosin V is responsible for thyroid hormone-dependent binding of primary endosomes to the microfilaments and suggest that this motor mediates the actin-based endocytosis of the type II iodothyronine deiodinase [2].
• Expression of the type II iodothyronine deiodinase in cultured rat astrocytes is selenium-dependent [3].
• Daily variations in type II iodothyronine deiodinase activity in the rat brain as controlled by the biological clock [4].
• It has been demonstrated that type II iodothyronine deiodinase is present in rat pineal gland, and the deiodinase activity markedly increases during the hours of darkness, primarily through beta-adrenergic mechanism [5].
• Daytime isoproterenol administration resulted in a rapid increase in pineal type II iodothyronine deiodinase mRNA followed by the increase in deiodinase activity [5].

Biological context of Dio2

• Evidence that cysteine, not selenocysteine, is in the catalytic site of type II iodothyronine deiodinase [6].
• Feeding of thiocyanate through gestation and lactation resulted in an increase in type II 5'deiodinase activity in cerebrum, cerebellum and brainstem of the 21 day old pups compared to controls [1].

Anatomical context of Dio2

• These results suggest that the rhythmic change in pineal type II iodothyronine deiodinase activity is regulated at least in part at the pretranslational level by a beta-adrenergic mechanism transmitted through superior cervical ganglia [5].
• Fasting-induced increase in type II iodothyronine deiodinase activity and messenger ribonucleic acid levels is not reversed by thyroxine in the rat hypothalamus [7].
• Northern analysis of type II iodothyronine deiodinase mRNA in rat Harderian gland [8].

Associations of Dio2 with chemical compounds

• Propranolol treatment, bilateral superior cervical ganglionectomy, or constant light exposure significantly suppressed the nocturnal rise in type II iodothyronine deiodinase mRNA as well as the deiodinase activity [5].
• Dibutyryl cAMP induction of type II 5'deiodinase activity in rat brain astrocytes in culture [9].
• Experiments were performed in weaning rats to understand the influence of thiocyanate, an hydrolytic product of glucosinolates present in foods, on the generation of T3 in situ by type II 5'deiodinase and the binding of 125I T3 to specific nuclear receptors in developing brain [1].
• The influence of hypothalamic and pituitary type II 5'deiodinase (5'D-II) activities and T3 content on pituitary TSH content was investigated in streptozotocin (STZ)-induced diabetic rats (D) [10].

Analytical, diagnostic and therapeutic context of Dio2

• Reverse transcription-polymerase chain reaction amplification and Northern blot analyses have demonstrated that type II iodothyronine deiodinase mRNA is expressed in rat pineal gland and its mRNA level increases markedly at midnight [11].

References