High impact information on SLC30A3

• ZnT-3, a putative transporter of zinc into synaptic vesicles [1].
• Northern blot and reverse transcriptase-PCR analyses demonstrate that murine ZnT-3 expression is restricted to the brain and testis [1].
• The mouse ZnT3 gene has 8 exons and maps to chromosome 5 [1].
• Human ZnT-3 cDNAs were also cloned; the amino acid sequence is 86% identical to murine ZnT-3 [1].
• In situ hybridization reveals that within the brain, ZnT-3 mRNA is most abundant in the hippocampus and cerebral cortex [1].

Biological context of SLC30A3

• Accordingly, pharmacological disruption of either AP-2- or AP-3-dependent SLMV biogenesis preferentially reduced synaptophysin or ZnT3 targeting, respectively; suggesting that these antigens were concentrated in different vesicles [2].
• However there appears to be little chelatable (synaptic) zinc in the cerebellum, deletion of the ZnT-3 gene has no effect on motor phenotype and there is currently no evidence that zinc is released from cerebellar neurones to have physiological actions [3].
• Over-expression of the zinc transporter 3 gene was identified as the most prominent change in gene expression due to omega-3 PUFA deficiency [4].
• The possibility that zinc is involved in cerebellar synaptic transmission is supported by the expression of specific zinc transporters (ZnT, including the synaptic vesicle transporter ZnT-3) in the cerebellar cortex [3].

Anatomical context of SLC30A3

• We detected expression of two other members of the hZnT family, hZnT1 and hZnT3, in human breast epithelial cells [5].
• ZnT-2 and ZnT-3 mRNAs were present in low to moderate levels in pancreatic islets of 10-day and 5-week-old rats, but were absent in 3-day-old pancreas and islets of adult animals [6].
• Zinc Transporter 3 (ZnT-3) mRNA expression was decreased in hippocampus (2.3-fold decrease, p= 0.0065); staining for its correlate protein was reduced in hippocampal mossy fibers [7].

Associations of SLC30A3 with chemical compounds

• Furthermore, the expression of ZnT3 mRNA was decreased by incubating LNCaP cells in medium containing hormone-stripped fetal calf serum and increased by addition of synthetic androgen R1881 to the medium, whereas the intracellular zinc levels were not affected under these conditions [8].
• The autometallography staining resulting from the "sulfide only immersion" was not particularly impressive, but the significance of this return to an old approach became obvious when Wenzel and co-workers presented their approach in connection with introduction by the Palmiter group of zinc transporter 3 (ZnT3) [9].

Other interactions of SLC30A3

• These findings suggest that factors such as ZnT1 and metallothioneins other than ZnT3 are associated with the low intracellular zinc content in AIDL cells [8].
• ZnT-2 and ZnT-3 may function in tissue-specific vesicular zinc transport [10].

References