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Gene Review

PLC1  -  phosphatidylinositol phospholipase C

Saccharomyces cerevisiae S288c

Synonyms: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1, PLC-1, Phosphoinositide phospholipase C, Phospholipase C-1, YPL268W
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Disease relevance of PLC1


High impact information on PLC1

  • Structure, function, and control of phosphoinositide-specific phospholipase C [5].
  • Phosphoinositide-specific phospholipase C (PLC) subtypes beta, gamma, and delta comprise a related group of multidomain phosphodiesterases that cleave the polar head groups from inositol lipids [5].
  • ABP1p is a novel protein with a 50 amino-acid C-terminal domain that is very similar to the SH3 domain in the non-catalytic region of nonreceptor tyrosine kinases (including those encoded by the proto-oncogenes c-src and c-abl), in phospholipase C gamma and in alpha-spectrin [6].
  • Phospholipase C and two inositol polyphosphate (IP) kinases constitute a signaling pathway that regulates nuclear messenger RNA export through production of inositol hexakisphosphate (IP6) [7].
  • Here, we show that in native cardiac cells and heterologous expression systems, G alpha q-linked receptors or tyrosine kinase receptors that activate PLC potently inhibit channel activity [8].

Chemical compound and disease context of PLC1

  • Phospholipase C from the Dallas 1E strain of Legionella pneumophila serogroup 5 was purified from buffered yeast extract culture supernate by ion-exchange chromatography followed by fractionation by manganous chloride and ammonium sulphate precipitation steps [3].

Biological context of PLC1


Anatomical context of PLC1


Associations of PLC1 with chemical compounds


Physical interactions of PLC1


Regulatory relationships of PLC1

  • Finally, a ume3 null allele suppresses the growth defect of plc1 mutants in response to either elevated temperature or the presence of hydrogen peroxide [18].

Other interactions of PLC1

  • Oxidative stress-induced destruction of the yeast C-type cyclin Ume3p requires phosphatidylinositol-specific phospholipase C and the 26S proteasome [18].
  • Surprisingly, overexpression of PLC1 and MSS4 also suppressed the growth defect of a class B mutant [22].
  • The hormone receptor-like protein Gpr1p physically interacts with phosphatidylinositol-specific phospholipase C (Plc1p) and with the Galpha protein Gpa2p, as shown by two-hybrid assays and co-immune precipitation of epitope-tagged proteins [20].
  • Deletion of PLC1, as well as plc1 mutations which abrogate the interaction of Plc1p with the CBF3 complex, results in a higher frequency of minichromosome loss, nocodazole sensitivity, and mitotic delay [23].
  • Furthermore, deletion of PLC1 in an inp51 mutant does not abrogate cold tolerance, indicating that Plc1p-mediated production of soluble inositol phosphates is not required [24].

Analytical, diagnostic and therapeutic context of PLC1


  1. A simple and reliable method for rapid production and purification of Pseudomonas aeruginosa haemolytic phospholipase C. Dubouix, A., Nieto, M., Fauvel, J., Chap, H., Marty, N., Salles, J.P., Gaits, F. Lett. Appl. Microbiol. (2004) [Pubmed]
  2. Incidence and identification of phospholipase C-producing bacteria in fresh and spoiled homogenized milk. Fox, C.W., Chrisope, G.L., Marshall, R.T. J. Dairy Sci. (1976) [Pubmed]
  3. A phospholipase C from the Dallas 1E strain of Legionella pneumophila serogroup 5: purification and characterization of conditions for optimal activity with an artificial substrate. Baine, W.B. J. Gen. Microbiol. (1988) [Pubmed]
  4. High-level expression of recombinant phospholipase C from Bacillus cereus in Pichia pastoris and its characterization. Seo, K.H., Rhee, J.I. Biotechnol. Lett. (2004) [Pubmed]
  5. Structure, function, and control of phosphoinositide-specific phospholipase C. Rebecchi, M.J., Pentyala, S.N. Physiol. Rev. (2000) [Pubmed]
  6. Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I. Drubin, D.G., Mulholland, J., Zhu, Z.M., Botstein, D. Nature (1990) [Pubmed]
  7. A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Odom, A.R., Stahlberg, A., Wente, S.R., York, J.D. Science (2000) [Pubmed]
  8. The TRPM7 channel is inactivated by PIP(2) hydrolysis. Runnels, L.W., Yue, L., Clapham, D.E. Nat. Cell Biol. (2002) [Pubmed]
  9. Genetic evidence for a role of phospholipase C at the budding yeast kinetochore. DeLillo, N., Romero, C., Lin, H., Vancura, A. Mol. Genet. Genomics (2003) [Pubmed]
  10. Evidence for inositol triphosphate as a second messenger for glucose-induced calcium signalling in budding yeast. Tisi, R., Belotti, F., Wera, S., Winderickx, J., Thevelein, J.M., Martegani, E. Curr. Genet. (2004) [Pubmed]
  11. Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae. Lin, H., Nguyen, P., Vancura, A. Mol. Genet. Genomics (2002) [Pubmed]
  12. The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth. Yoko-o, T., Matsui, Y., Yagisawa, H., Nojima, H., Uno, I., Toh-e, A. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  13. Diacylglycerol and its formation by phospholipase C regulate Rab- and SNARE-dependent yeast vacuole fusion. Jun, Y., Fratti, R.A., Wickner, W. J. Biol. Chem. (2004) [Pubmed]
  14. Characterization of a plasma membrane-associated phosphoinositide-specific phospholipase C from soybean. Shi, J., Gonzales, R.A., Bhattacharyya, M.K. Plant J. (1995) [Pubmed]
  15. Ca2+ signal is generated only once in the mating pheromone response pathway in Saccharomyces cerevisiae. Nakajima-Shimada, J., Sakaguchi, S., Tsuji, F.I., Anraku, Y., Iida, H. Cell Struct. Funct. (2000) [Pubmed]
  16. The yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase C-mediated inositol lipid signaling in cytosol-depleted RBL-2H3 and HL-60 cells. Cunningham, E., Tan, S.K., Swigart, P., Hsuan, J., Bankaitis, V., Cockcroft, S. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  17. Activation and localization of inositol phosphosphingolipid phospholipase C, Isc1p, to the mitochondria during growth of Saccharomyces cerevisiae. Vaena de Avalos, S., Okamoto, Y., Hannun, Y.A. J. Biol. Chem. (2004) [Pubmed]
  18. Oxidative stress-induced destruction of the yeast C-type cyclin Ume3p requires phosphatidylinositol-specific phospholipase C and the 26S proteasome. Cooper, K.F., Mallory, M.J., Strich, R. Mol. Cell. Biol. (1999) [Pubmed]
  19. Heat-induced elevation of ceramide in Saccharomyces cerevisiae via de novo synthesis. Wells, G.B., Dickson, R.C., Lester, R.L. J. Biol. Chem. (1998) [Pubmed]
  20. Phospholipase C binds to the receptor-like GPR1 protein and controls pseudohyphal differentiation in Saccharomyces cerevisiae. Ansari, K., Martin, S., Farkasovsky, M., Ehbrecht, I.M., Küntzel, H. J. Biol. Chem. (1999) [Pubmed]
  21. Phosphoinositide-specific phospholipase C interacts with phosphatidylinositol kinase homolog TOR2. Lin, H., Choi, J.H., Vancura, A. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  22. TOR2 is part of two related signaling pathways coordinating cell growth in Saccharomyces cerevisiae. Helliwell, S.B., Howald, I., Barbet, N., Hall, M.N. Genetics (1998) [Pubmed]
  23. Phospholipase C is involved in kinetochore function in Saccharomyces cerevisiae. Lin, H., Choi, J.H., Hasek, J., DeLillo, N., Lou, W., Vancura, A. Mol. Cell. Biol. (2000) [Pubmed]
  24. INP51, a yeast inositol polyphosphate 5-phosphatase required for phosphatidylinositol 4,5-bisphosphate homeostasis and whose absence confers a cold-resistant phenotype. Stolz, L.E., Kuo, W.J., Longchamps, J., Sekhon, M.K., York, J.D. J. Biol. Chem. (1998) [Pubmed]
  25. Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae. Flick, J.S., Thorner, J. Mol. Cell. Biol. (1993) [Pubmed]
  26. Molecular cloning of the plc1+ gene of Schizosaccharomyces pombe, which encodes a putative phosphoinositide-specific phospholipase C. Andoh, T., Yoko, T., Matsui, Y., Toh, A. Yeast (1995) [Pubmed]
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