Disease relevance of Ranavirus

• The greatest rates of phosphorylation were obtained with acidic phosphoprotein substrates such as casein or phosvitin, although potential physiological substrates for this activity included specific virion polypeptides of frog virus [1].
• Phylogenetic analysis of the major capsid protein, ATPase, cytosine DNA methyl transferase and DNA polymerase indicated that OSGIV was closely related to infectious spleen and kidney necrosis virus (ISKNV) and rock bream iridovirus (RBIV), but differed from lymphocytisvirus and ranavirus [2].
• Comparison of the eIF-2alpha homologous proteins of seven ranaviruses (Iridoviridae) [3].
• The genome of the large icosahedral DNA virus, frog virus 3 (FV3), is heavily methylated at the cytosine residues of dCdG dinucleotide pairs, with more than 22% of the total cytosine residues in the form of 5-methylcytosine (5mC) [4].

High impact information on Ranavirus

• The selective 5-lipoxygenase inhibitor AA 861 and the dual inhibitor of arachidonate lipoxygenase and cyclooxygenase, BW 755C, reduced the hepatocellular injury after a high dose of frog virus 3 by about 50 and 80%, respectively, as judged from plasma activities of ALT and sorbitol dehydrogenase at 24 hr after frog virus 3 administration [5].
• A fully 5'-CG-3' but not a 5'-CCGG-3' methylated late frog virus 3 promoter retains activity [6].
• As with the previously sequenced frog virus 3 immediate-early gene encoding ICR169, expression of chloramphenicol acetyltransferase in transfected cells required activation by a virion-associated protein [7].
• Nineteen frog virus 3 temperature-sensitive mutants were isolated after mutagenesis with nitrosoguanidine and assayed for viral DNA, RNA, and protein synthesis, as well as assembly site formation at permissive (25 degrees C) and nonpermissive (30 degrees C) temperatures [8].
• Over 20% of the cytosine bases in frog virus 3 DNA are methylated at the 5-carbon position [9].

Chemical compound and disease context of Ranavirus

• The eucaryotic polyadenylic acid addition signal (AATAAA) was not present, a finding to be expected since frog virus 3 mRNA is not polyadenylated [10].
• Arginine requirement for frog virus 3 development [11].
• Isolation and characterization of a frog virus 3 variant resistant to phosphonoacetate: genetic evidence for a virus-specific DNA polymerase [12].
• Infection with frog virus 3 allows transcription of DNA methylated at cytosine but not adenine residues [13].
• The role of DNA methylation in virus replication: inhibition of frog virus 3 replication by 5-azacytidine [14].

Biological context of Ranavirus

• Sequence analysis revealed open reading frames (ORF) sharing homologies with sequences from the Frog virus 3 such as the p31 and p40 proteins [15].

Anatomical context of Ranavirus

• Fate of frog virus 3 DNA replicated in the nucleus of arginine-deprived CHO cells [16].

Gene context of Ranavirus

• At least 12 virus-induced DNA-binding proteins with mol. wt. ranging from 14 X 10(3) have been isolated from frog virus (FV 3)-infected fathead minnow cells by DNA affinity chromatography [17].
• Rapid differentiation of Australian, European and American ranaviruses based on variation in major capsid protein gene sequence [18].
• Liver tissue samples were found positive for the virus by PCR amplification of the ranavirus (Family: Iridoviridae) major capsid protein (MCP) [19].

References