High impact information on Genes, T-Cell Receptor beta

• Accordingly, treatment of Ebeta-deleted thymocytes with the histone deacetylase inhibitor trichostatin A relieved the block in TCR-beta gene expression and promoted recombination within the Dbeta-Jbeta loci [1].
• Analysis of the resulting CD4+/CD8+ thymocytes revealed no surface expression of the TCR/CD3 complex and no V-D-J rearrangement of the TCR-beta gene locus [2].
• CD3 epsilon and CD3 zeta were expressed intracellularly 2 and 1 d, respectively, before intracellular expression of the TCR-beta chain, potentially allowing immediate surface expression of an immature TCR-beta-CD3 complex as soon as functional rearrangement of a TCR-beta gene locus has been accomplished [3].
• Here, we demonstrate that this is not because of promoter or cell type but instead is directed by regulatory sequences within the rearranging VDJ exon and immediately flanking intron sequences of a Vbeta8.1 TCR-beta gene [4].
• CHX also induces full length TCR-beta transcripts greater than 90-fold while TCR-beta gene transcription increases only 2- to 4-fold [5].

Biological context of Genes, T-Cell Receptor beta

• Together these data suggest that the p70 autoantigen binds a critical motif in the beta enhancer and probably regulates TCR beta gene expression [6].
• The expression of TRG gamma mRNA (transcripts of 1.6 kilobases [kb]) was highly variable from case to case and did not correlate with the stage of differentiation of the malignant cells, the expression of the molecules CD4 and CD8, the expression and size of the transcripts of the TCR beta genes, and the transcription of TCR alpha genes [7].
• These results represent a previously unreported disease association and suggest that there may be some form of epistatic interaction between the CF gene and the TCR beta genes such that the double heterozygote is immunologically advantaged [8].
• LD1 cells have deleted the TCR delta locus, have rearrangements at the TCR gamma gene cluster (i.e. a V gamma 1-J gamma 1-C gamma 1 and a V gamma 4.3-J gamma 4-C gamma 4) and have two rearrangements of the TCR beta gene cluster (i.e. a D beta 1-J beta 1 and V-D-J beta 2) [9].
• Gene rearrangements were studied in 10 cases for IgH and TCR beta genes and in 5 cases for the TRG gene [10].

Anatomical context of Genes, T-Cell Receptor beta

• CD3-epsilon-deficient thymocytes do rearrange their T cell receptor (TCR) beta gene segments and produce low levels of full-length TCR beta transcripts [11].
• Southern blot analysis of total PBL gamma cell lines and clones indicated that this major circulating subset of TCR gamma cells retained a TCR beta gene in germline configuration and preferentially expressed a single V gamma gene, V gamma 5 for patient 1 and V gamma 9 for patient 2 [12].
• The results presented here suggest that thymocytes may regulate GKLF transcriptionally in the cortical epithelium at the developmental check-point controlled by TCR beta gene rearrangement [13].
• One case of B-cell leukaemia showed a rearrangement of both TCR beta genes and JH genes [14].
• METHODS: The complementarity determining region 3 (CDR3) of the TCR beta gene with 24 variable regions was amplified in peripheral blood mononuclear cells drawn from one cGVHD patient after allogenic bone marrow transplantation (allo-BMT) 35, 39, 43 or 45 months respectively, using RT-PCR, to observe the expression of TCR V beta repertoire T cells [15].

Associations of Genes, T-Cell Receptor beta with chemical compounds

• The 1.6-kb TCR-alpha and the 1.0-kb D beta J beta C beta TCR-beta gene transcripts were fully induced in the presence of cycloheximide, whereas the 1.3-kb V beta D beta J beta C beta transcript was only partly induced in the presence of cycloheximide [16].
• The C gamma 1 gene segment, just like the TcR alpha and TcR beta gene segments, contains a cysteine codon in its second exon [17].
• Thirty-five TCCs specific for PPD or TT and restricted by HLA-Dw4 and/or -Dw14.1 were selected for TCR beta gene sequencing [18].
• The mutation in TCR beta gene affected the frequency of nucleotide insertions at the V delta-D delta signal joints and inhibited the formation of replacement joint [19].

Gene context of Genes, T-Cell Receptor beta

• All samples expressed CD5, CD19 and CD20 and six patients had rearrangements of both immunoglobulin and TcR beta genes [20].
• These observations suggest that the coordinate expressions of CD2 and TCR-beta genes share common regulatory mechanisms involving factors regulating chromatin structure and accessibility [21].
• Moreover, the expression of the TCR beta gene was suppressed by IFN-beta under Con A stimulation, suggesting that IFN-beta might work as a negative regulator in immune recognition by activated T cells [22].
• Survival of pAML patients with TcR-beta gene rearrangements was longer and survival of pAML patients with IL-3 or GM-CSF gene rearrangement was shorter than in patients without those rearrangements [23].
• High overall diversity in the TCR alpha and TCR beta genes used for recognition of this self-Ag, MBP, was observed [24].

References