Disease relevance of vitamin H

• We demonstrate here that endodermal sinus tumour cells, but not other cell types isolated from testicular teratomas, secrete the serum attachment protein, vitronectin (also known as serum-spreading factor, S-protein or epibolin), as well as fibronectin, laminin and type IV collagen, into serum-free medium [1].
• Because restoration of pimaricin production in the npi287 mutant is conferred by both A-factor and PI factor, S. natalensis appears to be able to integrate different quorum signals from actinomycetes [2].
• Membranes containing permease with the biotin acceptor domain from the Klebsiella pneumoniae oxaloacetate decarboxylase are extracted with 5 M urea or treated with dicyclohexylcarbodiimide to inactivate F1/Fo ATPase and biotinylated in vitro with biotin ligase, ATP and d-biotin [3].
• Rhodobacter sphaeroides f. sp. denitrificans biotin sulfoxide reductase (BSOR) catalyzes the reduction of d-biotin d-sulfoxide to biotin and contains the molybdopterin guanine dinucleotide (MGD) cofactor as its sole prosthetic group [4].
• With the parameters (such as k1, k2, growth rate constant kG, critical concentration of HOCs in the organism resulting in growth inhibition CA* and spread factor S) the variability in toxicity (such as EC10, EC50, EC70) can be estimated from the combined bioconcentration and probability model, which fits well with the experimental observations [5].

Psychiatry related information on vitamin H

• Slow-wave sleep (SWS) and rapid-eye-movement sleep (REM) were recorded in cats for 32 h a) under control conditions, b) following intraventricular infusions of artificial cerebrospinal fluid (CSF), and c) following infusions of sleep-promoting factor S prepared from human urine (SPU) [6].
• Factor S also induced increases in EEG slow-wave (1/2-4 Hz) amplitudes similar to those observed during the deep sleep that follows sleep deprivation [7].

High impact information on vitamin H

• According to a recently proposed theory for hindered diffusion through fibrous media, the diffusivity ratio is given by the product of a hydrodynamic factor (F) and a steric factor (S) [8].
• It is now known that epibolin is identical to serum spreading factor, S protein, and vitronectin, and the current name for the protein is vitronectin [9].
• Specular microscopy and computer-assisted morphometry was used to quantify central and peripheral endothelial cell density (ECD), coefficient of variation (COV) and the mean and standard deviation of the shape factor (S) over an 18-month period [10].
• An efficient synthesis of a terminal alkyne derived from d-biotin was developed to provide an alternative for carboxyl-based biotinylation [11].
• Extraction of sleep-promoting factor S from cerebrospinal fluid and from brains of sleep-deprived animals [12].

Biological context of vitamin H

• Eight species of novel recombinant tumor necrosis factor-S (rTNF-SAM group) were constructed in which N-terminal amino acid sequences were based on that of TNF-S from THP-1 cells with higher basicity than conventional rTNF-alpha [13].
• The coenzyme d-biotin offers in its anionic form to metal ions 3 possible binding sites: the carboxylate group of the valerate side chain, the ureido residue of the 2-imidazolidone ring, and the thioether sulfur of the tetrahydrothiophene ring; the coordinating properties of these groups are summarized and compared [14].
• To induce cell differentiation, undifferentiated 3T3-L1 cells were treated with dexamethasone, 1-methyl-3-isobutylxanthine, and d-biotin [15].

Anatomical context of vitamin H

• Sleep-promoting factor (factor S) was extracted, partially purified, and concentrated from cerebrospinal fluid and from acid-acetone extracts of brain stem anc cortex of sleep-deprived goats and sheep [12].
• By pull-down assays we demonstrate that SA covalently bound to the ribosome binds d-biotin [16].
• Two new non-BoLA antigens were also detected, one completely included within the red blood cell factor S' (BoLy-S'), whereas a second (BoLy-w1) did not show any association with tested red blood cell factors [17].

Gene context of vitamin H

• In contrast, transcripts for Growth/ Differentiation factor-S (GDF-5) were induced during a cellular condensation, and those for BMP-6 were induced during the formation of cartilage nodules, and declined as the differentiated ATDC5 cells became hypertrophic, and BMP-7 transcripts were only detected after cells became calcified [18].
• Biotin sulfoxide reductase catalyzes the conversion of d-biotin d-sulfoxide (BSO) to d-biotin [19].
• The JAS was scored in the traditional manner, yielding scores for ambitiousness (factor A), impatience (factor S), competitiveness (factor H) and job involvement (factor J) [20].
• As determined by Sephadex G-50 chromatography, only an 8-fold molar excess of d-biotin relative to labelled streptavidin was required to displace 90% of label in the case of EB1, whereas even a 20-fold molar excess provided no detectable displacement of DB2 [21].
• At 25 days of incubation, eggs from Large White turkey hens in two field trials were injected with 87 micrograms of exogenous d-biotin per egg [22].

Analytical, diagnostic and therapeutic context of vitamin H

• A competitive enzyme immunoassay was used to study the binding of biotinylated macromolecular ligands and d-biotin to an anti-biotin monoclonal antibody and to streptavidin [23].
• The behavior of the urinary factor in two ion exchange chromatographic steps, high voltage electrophoresis, gel-filtration, and ultrafiltration was similar to that of factor S. Effects of the purified urinary factor on slow-wave sleep of rats and rabbits were similar in time-course and duration to those of factor S from brain [24].

References